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Notch1 is associated with the differentiation of human bone marrow‑derived mesenchymal stem cells to cardiomyocytes

  • Authors:
    • Zipu Yu
    • Yu Zou
    • Jingya Fan
    • Chengchen Li
    • Liang Ma
  • View Affiliations / Copyright

    Affiliations: Department of Cardiac Surgery, First Affiliated Hospital, Zhejiang University, Hangzhou, Zhejiang 310000, P.R. China
    Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5065-5071
    |
    Published online on: October 19, 2016
       https://doi.org/10.3892/mmr.2016.5862
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Abstract

Notch signaling is involved in the early process of differentiation to determine the fate of stem cells. However, the precise role of Notch in human bone marrow‑derived mesenchymal stem cells (hBMSCs) remains unclear. The present study aimed to investigate the involvement of Notch signalling during the course of hBMSC differentiation into cardiomyocytes using hBMSCs, with multilineage differentiation ability, isolated and purified from human bone marrow. Flow cytometric analysis revealed that CD29, CD44 and CD90 were highly expressed on the surface of cells in their fifth passage, whereas detection of CD34, CD45, CD54 and HLA‑DR was negative. Visualization of morphological changes, western blotting, immunocytochemistry and reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) demonstrated that hBMSCs differentiate into cardiomyocytes through treatment with 5‑azacytidine (5‑aza). Transmission electron microscopy revealed ultramicroscopic details of differentiated hBMSCs. Western blotting and immunocytochemistry demonstrated increased protein expression levels of α‑actin and cardiac troponin T expression, and RT‑qPCR revealed increased mRNA expression of Notch1 early in the process of differentiation (days 1, 4 and 7), and increased mRNA expression levels of the transcription factors GATA binding protein‑4 and NK2 homeobox 5 at day 28 day. In conclusion, differentiation of hBMSCs into cardiomyocytes was induced in vitro by 5‑aza, and was associated with upregulation of Notch1, GATA binding protein‑4 and Nkx2.5 expression. Overexpression of the Notch1 signaling pathway may represent a potential mechanism underlying the differentiation of hBMSCs.
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Copy and paste a formatted citation
Spandidos Publications style
Yu Z, Zou Y, Fan J, Li C and Ma L: Notch1 is associated with the differentiation of human bone marrow‑derived mesenchymal stem cells to cardiomyocytes. Mol Med Rep 14: 5065-5071, 2016.
APA
Yu, Z., Zou, Y., Fan, J., Li, C., & Ma, L. (2016). Notch1 is associated with the differentiation of human bone marrow‑derived mesenchymal stem cells to cardiomyocytes. Molecular Medicine Reports, 14, 5065-5071. https://doi.org/10.3892/mmr.2016.5862
MLA
Yu, Z., Zou, Y., Fan, J., Li, C., Ma, L."Notch1 is associated with the differentiation of human bone marrow‑derived mesenchymal stem cells to cardiomyocytes". Molecular Medicine Reports 14.6 (2016): 5065-5071.
Chicago
Yu, Z., Zou, Y., Fan, J., Li, C., Ma, L."Notch1 is associated with the differentiation of human bone marrow‑derived mesenchymal stem cells to cardiomyocytes". Molecular Medicine Reports 14, no. 6 (2016): 5065-5071. https://doi.org/10.3892/mmr.2016.5862
Copy and paste a formatted citation
x
Spandidos Publications style
Yu Z, Zou Y, Fan J, Li C and Ma L: Notch1 is associated with the differentiation of human bone marrow‑derived mesenchymal stem cells to cardiomyocytes. Mol Med Rep 14: 5065-5071, 2016.
APA
Yu, Z., Zou, Y., Fan, J., Li, C., & Ma, L. (2016). Notch1 is associated with the differentiation of human bone marrow‑derived mesenchymal stem cells to cardiomyocytes. Molecular Medicine Reports, 14, 5065-5071. https://doi.org/10.3892/mmr.2016.5862
MLA
Yu, Z., Zou, Y., Fan, J., Li, C., Ma, L."Notch1 is associated with the differentiation of human bone marrow‑derived mesenchymal stem cells to cardiomyocytes". Molecular Medicine Reports 14.6 (2016): 5065-5071.
Chicago
Yu, Z., Zou, Y., Fan, J., Li, C., Ma, L."Notch1 is associated with the differentiation of human bone marrow‑derived mesenchymal stem cells to cardiomyocytes". Molecular Medicine Reports 14, no. 6 (2016): 5065-5071. https://doi.org/10.3892/mmr.2016.5862
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