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Suppressive effects of Gua Lou Gui Zhi decoction on MCAO-induced NO and PGE2 production are dependent on the MAPK and NF-κB signaling pathways

  • Authors:
    • Haixia Hu
    • Xiaoqin Zhu
    • Ruhui Lin
    • Zuanfang Li
    • Lidian Chen
  • View Affiliations / Copyright

    Affiliations: Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350108, P.R. China, College of Rehabilitation Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350108, P.R. China
    Copyright: © Hu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5141-5147
    |
    Published online on: October 21, 2016
       https://doi.org/10.3892/mmr.2016.5876
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Abstract

The present study aimed to investigate the inhibitory effects, and underlying mechanisms, of Gua Lou Gui Zhi decoction (GLGZD) in a rat model of neuroinflammation. Sprague-Dawley rats were treated with GLGZD following middle cerebral artery occlusion (MCAO). Neurological function and infarct volume were evaluated to confirm successful generation of the rat model. Subsequently, brain tissues and blood samples were collected for further analysis. Nitric oxide (NO) and prostaglandin E2 (PGE2) were evaluated in peripheral blood samples using the Griess reagent assay and an ELISA, respectively. The relative expression levels of inducible nitric oxide synthase (iNOS) and cylooxygenase‑2 (COX‑2) were detected by quantitative polymerase chain reaction and immunohistochemistry. The associated pathways, including nuclear factor‑κB (NF‑κB) and mitogen‑activated protein kinases (MAPK) signaling pathways, were detected by electrophoretic mobility shift assay and western blotting. The results demonstrated that treatment with GLGZD significantly inhibited MCAO-induced inflammation; GLGZD suppressed the production of NO and PGE2, and the expression of iNOS and COX‑2, by inhibiting NF‑κB activation and MAPK phosphorylation. These findings suggest that GLGZD, a potential agent for post‑stroke treatment, may exert anti‑inflammatory effects, thus providing neuroprotection.
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Copy and paste a formatted citation
Spandidos Publications style
Hu H, Zhu X, Lin R, Li Z and Chen L: Suppressive effects of Gua Lou Gui Zhi decoction on MCAO-induced NO and PGE2 production are dependent on the MAPK and NF-κB signaling pathways. Mol Med Rep 14: 5141-5147, 2016.
APA
Hu, H., Zhu, X., Lin, R., Li, Z., & Chen, L. (2016). Suppressive effects of Gua Lou Gui Zhi decoction on MCAO-induced NO and PGE2 production are dependent on the MAPK and NF-κB signaling pathways. Molecular Medicine Reports, 14, 5141-5147. https://doi.org/10.3892/mmr.2016.5876
MLA
Hu, H., Zhu, X., Lin, R., Li, Z., Chen, L."Suppressive effects of Gua Lou Gui Zhi decoction on MCAO-induced NO and PGE2 production are dependent on the MAPK and NF-κB signaling pathways". Molecular Medicine Reports 14.6 (2016): 5141-5147.
Chicago
Hu, H., Zhu, X., Lin, R., Li, Z., Chen, L."Suppressive effects of Gua Lou Gui Zhi decoction on MCAO-induced NO and PGE2 production are dependent on the MAPK and NF-κB signaling pathways". Molecular Medicine Reports 14, no. 6 (2016): 5141-5147. https://doi.org/10.3892/mmr.2016.5876
Copy and paste a formatted citation
x
Spandidos Publications style
Hu H, Zhu X, Lin R, Li Z and Chen L: Suppressive effects of Gua Lou Gui Zhi decoction on MCAO-induced NO and PGE2 production are dependent on the MAPK and NF-κB signaling pathways. Mol Med Rep 14: 5141-5147, 2016.
APA
Hu, H., Zhu, X., Lin, R., Li, Z., & Chen, L. (2016). Suppressive effects of Gua Lou Gui Zhi decoction on MCAO-induced NO and PGE2 production are dependent on the MAPK and NF-κB signaling pathways. Molecular Medicine Reports, 14, 5141-5147. https://doi.org/10.3892/mmr.2016.5876
MLA
Hu, H., Zhu, X., Lin, R., Li, Z., Chen, L."Suppressive effects of Gua Lou Gui Zhi decoction on MCAO-induced NO and PGE2 production are dependent on the MAPK and NF-κB signaling pathways". Molecular Medicine Reports 14.6 (2016): 5141-5147.
Chicago
Hu, H., Zhu, X., Lin, R., Li, Z., Chen, L."Suppressive effects of Gua Lou Gui Zhi decoction on MCAO-induced NO and PGE2 production are dependent on the MAPK and NF-κB signaling pathways". Molecular Medicine Reports 14, no. 6 (2016): 5141-5147. https://doi.org/10.3892/mmr.2016.5876
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