Open Access

Extracts containing CLPs of Bacillus amyloliquefaciens JN68 isolated from chicken intestines exert antimicrobial effects, particularly on methicillin-resistant Staphylococcus aureus and Listeria monocytogenes

  • Authors:
    • Jen‑Ni Chen
    • Chyou‑Wei Wei
    • Hsiao‑Chun Liu
    • Shu‑Ying Chen
    • Chinshuh Chen
    • Yu‑Min Juang
    • Chien‑Chen Lai
    • Giou‑Teng Yiang
  • View Affiliations

  • Published online on: October 27, 2016     https://doi.org/10.3892/mmr.2016.5900
  • Pages: 5155-5163
  • Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Bacillus amyloliquefaciens JN68, which has been discussed with regards to its antimicrobial activities, was successfully isolated from healthy chicken intestines in the present study. Using the spot-on-the-lawn antagonism method, the preliminary study indicated that a suspension culture of the B. amyloliquefaciens JN68 strain can inhibit the growth of Aspergillus niger and Penicillium pinophilum. Furthermore, the cyclic lipopeptides (CLPs) produced by the B. amyloliquefaciens JN68 strain were further purified through acid precipitation and Bond Elut®C18 chromatography, and their structures were identified using the liquid chromatography‑electrospray ionization‑mass spectrometry (MS)/MS method. Purified CLPs exerted broad spectrum antimicrobial activities on various pathogenic and foodborne bacteria and fungi, as determined using the agar well diffusion method. Listeria monocytogenes can induce listeriosis, which is associated with a high mortality rate. Methicillin‑resistant Staphylococcus aureus (MRSA) is a major pathogenic bacteria that causes nosocomial infections. Therefore, L. monocytogenes and MRSA are currently of great concern. The present study aimed to determine whether B. amyloliquefaciens JN68 extracts could inhibit L. monocytogenes and MRSA. The results indicated that extracts of B. amyloliquefaciens JN68 have CLP components, and can successfully inhibit the growth of L. monocytogenes and MRSA.
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December-2016
Volume 14 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Chen JN, Wei CW, Liu HC, Chen SY, Chen C, Juang YM, Lai CC and Yiang GT: Extracts containing CLPs of Bacillus amyloliquefaciens JN68 isolated from chicken intestines exert antimicrobial effects, particularly on methicillin-resistant Staphylococcus aureus and Listeria monocytogenes. Mol Med Rep 14: 5155-5163, 2016
APA
Chen, J., Wei, C., Liu, H., Chen, S., Chen, C., Juang, Y. ... Yiang, G. (2016). Extracts containing CLPs of Bacillus amyloliquefaciens JN68 isolated from chicken intestines exert antimicrobial effects, particularly on methicillin-resistant Staphylococcus aureus and Listeria monocytogenes. Molecular Medicine Reports, 14, 5155-5163. https://doi.org/10.3892/mmr.2016.5900
MLA
Chen, J., Wei, C., Liu, H., Chen, S., Chen, C., Juang, Y., Lai, C., Yiang, G."Extracts containing CLPs of Bacillus amyloliquefaciens JN68 isolated from chicken intestines exert antimicrobial effects, particularly on methicillin-resistant Staphylococcus aureus and Listeria monocytogenes". Molecular Medicine Reports 14.6 (2016): 5155-5163.
Chicago
Chen, J., Wei, C., Liu, H., Chen, S., Chen, C., Juang, Y., Lai, C., Yiang, G."Extracts containing CLPs of Bacillus amyloliquefaciens JN68 isolated from chicken intestines exert antimicrobial effects, particularly on methicillin-resistant Staphylococcus aureus and Listeria monocytogenes". Molecular Medicine Reports 14, no. 6 (2016): 5155-5163. https://doi.org/10.3892/mmr.2016.5900