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Article

GRIN3A and MAPT stimulate nerve overgrowth in macrodactyly

  • Authors:
    • Xu Shi
    • Lu Lu
    • Xiu Jin
    • Bin Liu
    • Xiguang Sun
    • Laijin Lu
    • Yanfang Jiang
  • View Affiliations / Copyright

    Affiliations: Department of Genetic Diagnosis Center, Central Laboratory, The First Hospital of Jilin University, Changchun, Jilin 130000, P.R. China, Department of Breast Surgery, The First Hospital of Jilin University, Changchun, Jilin 130000, P.R. China, Department of Burn Surgery, The First Hospital of Jilin University, Changchun, Jilin 130000, P.R. China, Department of Hand and Foot Surgery, The First Hospital of Jilin University, Changchun, Jilin 130000, P.R. China
  • Pages: 5637-5643
    |
    Published online on: November 3, 2016
       https://doi.org/10.3892/mmr.2016.5923
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Abstract

As an uncommon and congenital condition, macrodactyly is characterized by an increase in the size of all the elements or structures of the digits or toes; however, the underlying pathogenesis remains to be fully elucidated. In the present study, the gene expression profiles of abnormal nerves were examined in three patients with macrodactyly using microarray analysis to identify potential genes contributing to nerve overgrowth. Gene expression profiling in the nerve tissue samples were scanned using the microarray and the differentially expressed genes were verified at the transcription level using reverse transcription‑quantitative polymerase chain reaction analysis. Western blot analysis was used to determine the expression of target genes at the translational level. To confirm the upregulated genes during the process of nerve proliferation, SH‑SY5Y cells were induced to differentiate into a neuronal‑like phenotype using retinoic acid. A total of 165 genes showed significant changes (≥5‑fold) in gene expression, which may be associated with the development of limbs in macrodactyly. Glutamate ionotropic receptor NMDA 3A (GRIN3A) and microtubule‑associated protein tau (MAPT) were identified as important contributors in promoting nerve overgrowth. Furthermore, it was identified that GRIN3A and MAPT were regulated by the cAMP‑protein kinase A and extracellular signal‑regulated kinase 1/2 pathways, respectively. The identification of genes expressed at high levels in macrodactyly may reveal potential factors, which contribute to abnormal nerve proliferation and underpin the pathogenesis of macrodactyly, and provide potential application targets in nerve tissue regeneration engineering.
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Copy and paste a formatted citation
Spandidos Publications style
Shi X, Lu L, Jin X, Liu B, Sun X, Lu L and Jiang Y: GRIN3A and MAPT stimulate nerve overgrowth in macrodactyly. Mol Med Rep 14: 5637-5643, 2016.
APA
Shi, X., Lu, L., Jin, X., Liu, B., Sun, X., Lu, L., & Jiang, Y. (2016). GRIN3A and MAPT stimulate nerve overgrowth in macrodactyly. Molecular Medicine Reports, 14, 5637-5643. https://doi.org/10.3892/mmr.2016.5923
MLA
Shi, X., Lu, L., Jin, X., Liu, B., Sun, X., Lu, L., Jiang, Y."GRIN3A and MAPT stimulate nerve overgrowth in macrodactyly". Molecular Medicine Reports 14.6 (2016): 5637-5643.
Chicago
Shi, X., Lu, L., Jin, X., Liu, B., Sun, X., Lu, L., Jiang, Y."GRIN3A and MAPT stimulate nerve overgrowth in macrodactyly". Molecular Medicine Reports 14, no. 6 (2016): 5637-5643. https://doi.org/10.3892/mmr.2016.5923
Copy and paste a formatted citation
x
Spandidos Publications style
Shi X, Lu L, Jin X, Liu B, Sun X, Lu L and Jiang Y: GRIN3A and MAPT stimulate nerve overgrowth in macrodactyly. Mol Med Rep 14: 5637-5643, 2016.
APA
Shi, X., Lu, L., Jin, X., Liu, B., Sun, X., Lu, L., & Jiang, Y. (2016). GRIN3A and MAPT stimulate nerve overgrowth in macrodactyly. Molecular Medicine Reports, 14, 5637-5643. https://doi.org/10.3892/mmr.2016.5923
MLA
Shi, X., Lu, L., Jin, X., Liu, B., Sun, X., Lu, L., Jiang, Y."GRIN3A and MAPT stimulate nerve overgrowth in macrodactyly". Molecular Medicine Reports 14.6 (2016): 5637-5643.
Chicago
Shi, X., Lu, L., Jin, X., Liu, B., Sun, X., Lu, L., Jiang, Y."GRIN3A and MAPT stimulate nerve overgrowth in macrodactyly". Molecular Medicine Reports 14, no. 6 (2016): 5637-5643. https://doi.org/10.3892/mmr.2016.5923
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