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Article

Selection of suitable reference genes for gene expression studies in normal human ovarian tissues, borderline ovarian tumours and ovarian cancer

  • Authors:
    • Olumide Ofinran
    • Ujjal Bose
    • Daniel Hay
    • Summi Abdul
    • Cristina Tufatelli
    • Raheela Khan
  • View Affiliations / Copyright

    Affiliations: Division of Medical Sciences and Graduate Entry Medicine, School of Medicine, The University of Nottingham, Royal Derby Hospital, Derby DE22 3DT, UK, Department of Gynaecological Oncology, Royal Derby Hospital, Derby DE22 3NE, UK
  • Pages: 5725-5731
    |
    Published online on: November 8, 2016
       https://doi.org/10.3892/mmr.2016.5933
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Abstract

The use of reference genes is the most common method of controlling the variation in mRNA expression during quantitative polymerase chain reaction, although the use of traditional reference genes, such as β‑actin, glyceraldehyde‑3‑phosphate dehydrogenase or 18S ribosomal RNA, without validation occasionally leads to unreliable results. Therefore, the present study aimed to evaluate a set of five commonly used reference genes to determine the most suitable for gene expression studies in normal ovarian tissues, borderline ovarian and ovarian cancer tissues. The expression stabilities of these genes were ranked using two gene stability algorithms, geNorm and NormFinder. Using geNorm, the two best reference genes in ovarian cancer were β‑glucuronidase and β‑actin. Hypoxanthine phosphoribosyltransferase‑1 and β‑glucuronidase were the most stable in ovarian borderline tumours, and hypoxanthine phosphoribosyltransferase‑1 and glyceraldehyde‑3‑phosphate dehydrogenase were the most stable in normal ovarian tissues. NormFinder ranked β‑actin the most stable in ovarian cancer, and the best combination of two genes was β‑glucuronidase and β‑actin. In borderline tumours, hypoxanthine phosphoribosyltransferase‑1 was identified as the most stable, and the best combination was hypoxanthine phosphoribosyltransferase‑1 and β‑glucuronidase. In normal ovarian tissues, β‑glucuronidase was recommended as the optimum reference gene, and the most optimum pair of reference genes was hypoxanthine phosphoribosyltransferase‑1 and β‑actin. To the best of our knowledge, this is the first study to investigate the selection of a set of reference genes for normalisation in quantitative polymerase chain reactions in different ovarian tissues, and therefore it is recommended that β‑glucuronidase, β‑actin and hypoxanthine phosphoribosyltransferase‑1 are the most suitable reference genes for such analyses.
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Copy and paste a formatted citation
Spandidos Publications style
Ofinran O, Bose U, Hay D, Abdul S, Tufatelli C and Khan R: Selection of suitable reference genes for gene expression studies in normal human ovarian tissues, borderline ovarian tumours and ovarian cancer. Mol Med Rep 14: 5725-5731, 2016.
APA
Ofinran, O., Bose, U., Hay, D., Abdul, S., Tufatelli, C., & Khan, R. (2016). Selection of suitable reference genes for gene expression studies in normal human ovarian tissues, borderline ovarian tumours and ovarian cancer. Molecular Medicine Reports, 14, 5725-5731. https://doi.org/10.3892/mmr.2016.5933
MLA
Ofinran, O., Bose, U., Hay, D., Abdul, S., Tufatelli, C., Khan, R."Selection of suitable reference genes for gene expression studies in normal human ovarian tissues, borderline ovarian tumours and ovarian cancer". Molecular Medicine Reports 14.6 (2016): 5725-5731.
Chicago
Ofinran, O., Bose, U., Hay, D., Abdul, S., Tufatelli, C., Khan, R."Selection of suitable reference genes for gene expression studies in normal human ovarian tissues, borderline ovarian tumours and ovarian cancer". Molecular Medicine Reports 14, no. 6 (2016): 5725-5731. https://doi.org/10.3892/mmr.2016.5933
Copy and paste a formatted citation
x
Spandidos Publications style
Ofinran O, Bose U, Hay D, Abdul S, Tufatelli C and Khan R: Selection of suitable reference genes for gene expression studies in normal human ovarian tissues, borderline ovarian tumours and ovarian cancer. Mol Med Rep 14: 5725-5731, 2016.
APA
Ofinran, O., Bose, U., Hay, D., Abdul, S., Tufatelli, C., & Khan, R. (2016). Selection of suitable reference genes for gene expression studies in normal human ovarian tissues, borderline ovarian tumours and ovarian cancer. Molecular Medicine Reports, 14, 5725-5731. https://doi.org/10.3892/mmr.2016.5933
MLA
Ofinran, O., Bose, U., Hay, D., Abdul, S., Tufatelli, C., Khan, R."Selection of suitable reference genes for gene expression studies in normal human ovarian tissues, borderline ovarian tumours and ovarian cancer". Molecular Medicine Reports 14.6 (2016): 5725-5731.
Chicago
Ofinran, O., Bose, U., Hay, D., Abdul, S., Tufatelli, C., Khan, R."Selection of suitable reference genes for gene expression studies in normal human ovarian tissues, borderline ovarian tumours and ovarian cancer". Molecular Medicine Reports 14, no. 6 (2016): 5725-5731. https://doi.org/10.3892/mmr.2016.5933
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