Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
December-2016 Volume 14 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
December-2016 Volume 14 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Role of perivascular adipose tissue in nicotine‑induced endothelial cell inflammatory responses

  • Authors:
    • Chao‑Nan Wang
    • Gen‑Huan Yang
    • Zhan‑Qi Wang
    • Chang‑Wei Liu
    • Tian‑Jia Li
    • Zhi‑Chao Lai
    • Shi‑Ying Miao
    • Lin‑Fang Wang
    • Bao Liu
  • View Affiliations / Copyright

    Affiliations: Department of Vascular Surgery, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100730, P.R. China, State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100730, P.R. China
  • Pages: 5713-5718
    |
    Published online on: November 9, 2016
       https://doi.org/10.3892/mmr.2016.5934
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Smoking is considered to be one of the primary causes of atherosclerosis and vascular injury. Previous studies have shown that nicotine in tobacco can lead to vascular inflammation and endothelial dysfunction. Perivascular adipose tissue (PVAT) is known to secrete various types of adipokines to maintain vascular homeostasis. The present study investigated whether nicotine‑induced PVAT malfunction can accelerate endothelial inflammation and eventually lead to endothelial dysfunction. The levels of inflammatory adipokines, including nuclear factor (NF)‑κB, interleukin (IL)‑1β, IL‑6 and tumor necrosis factor (TNF)‑α, the ICAM‑1 and VCAM‑1 adhesion molecules and secretion of adiponectin were assessed in mature adipocytes and endothelial cells cultured alone or in co‑culture under nicotine stimulation. It was found that nicotine reduced the secretion of adiponectin and stimulated secretion of the NF‑κB, IL‑1β, IL‑6 and TNF‑α inflammatory adipokines in mature adipocytes. Although nicotine stimulated endothelial cells to secrete IL‑1β and IL‑6, no significant increase in the secretion of TNF‑α was observed. The co‑culture of mature adipocytes with endothelial cells markedly augmented the expression of the NF‑κB, IL‑1β, IL‑6 and TNF‑α inflammatory adipokines and the ICAM‑1 and VCAM‑1 adhesion molecules, and significantly lowered the levels of adiponectin. These findings suggested that nicotine induced mature adipocyte dysfunction, which caused the abnormal secretion of adiponectin and inflammatory adipokines, and exacerbated endothelial inflammation. These findings also suggested a mechanism whereby nicotine induced the secretion of adiponectin and inflammatory cytokines by adipocytes. The results of the present study elucidated a novel pathway induced by cigarette smoke, which contributed to atherosclerosis and vascular injury.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

View References

1 

Ambrose JA and Barua RS: The pathophysiology of cigarette smoking and cardiovascular disease: An update. J Am Coll Cardiol. 43:1731–1737. 2004. View Article : Google Scholar : PubMed/NCBI

2 

Borgerding M and Klus H: Analysis of complex mixtures-cigarette smoke. Exp Toxicol Pathol 57 (Suppl 1). 43–73. 2005. View Article : Google Scholar

3 

Lee J, Taneja V and Vassallo R: Cigarette smoking and inflammation: Cellular and molecular mechanisms. J Dent Res. 91:142–149. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Orosz Z, Csiszar A, Labinskyy N, Smith K, Kaminski PM, Ferdinandy P, Wolin MS, Rivera A and Ungvari Z: Cigarette smoke-induced proinflammatory alterations in the endothelial phenotype: Role of NAD (P)H oxidase activation. Am J Physiol Heart Circ Physiol. 292:H130–H139. 2007. View Article : Google Scholar : PubMed/NCBI

5 

Arnson Y, Shoenfeld Y and Amital H: Effects of tobacco smoke on immunity, inflammation and autoimmunity. J Autoimmun. 34:J258–J265. 2010. View Article : Google Scholar : PubMed/NCBI

6 

Kuhlmann CR, Trümper JR, Tillmanns H, Schaefer C Alexander and Erdogan A: Nicotine inhibits large conductance Ca(2+)-activated K(+) channels and the NO/−cGMP signaling pathway in cultured human endothelial cells. Scand Cardiovasc J. 39:348–352. 2005. View Article : Google Scholar : PubMed/NCBI

7 

Glantz SA and Parmley WW: Passive and active smoking. A problem for adults. Circulation. 94:596–598. 1996. View Article : Google Scholar : PubMed/NCBI

8 

Halberg N, Wernstedt-Asterholm I and Scherer PE: The adipocyte as an endocrine cell. Endocrinol Metab Clin North Am. 37:753–768, x-xi. 2008. View Article : Google Scholar : PubMed/NCBI

9 

Lehr S, Hartwig S and Sell H: Adipokines: A treasure trove for the discovery of biomarkers for metabolic disorders. Proteomics Clin Appl. 6:91–101. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Kumada M, Kihara S, Sumitsuji S, Kawamoto T, Matsumoto S, Ouchi N, Arita Y, Okamoto Y, Shimomura I, Hiraoka H, et al: Association of hypoadiponectinemia with coronary artery disease in men. Arterioscler Thromb Vasc Biol. 23:85–89. 2003. View Article : Google Scholar : PubMed/NCBI

11 

Adamczak M, Wiecek A, Funahashi T, Chudek J, Kokot F and Matsuzawa Y: Decreased plasma adiponectin concentration in patients with essential hypertension. Am J Hypertens. 16:72–75. 2003. View Article : Google Scholar : PubMed/NCBI

12 

Ouchi N, Ohishi M, Kihara S, Funahashi T, Nakamura T, Nagaretani H, Kumada M, Ohashi K, Okamoto Y, Nishizawa H, et al: Association of hypoadiponectinemia with impaired vasoreactivity. Hypertension. 42:231–234. 2003. View Article : Google Scholar : PubMed/NCBI

13 

Van de Voorde J, Pauwels B, Boydens C and Decaluwé K: Adipocytokines in relation to cardiovascular disease. Metabolism. 62:1513–1521. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Suganami T, Tanimoto-Koyama K, Nishida J, Itoh M, Yuan X, Mizuarai S, Kotani H, Yamaoka S, Miyake K, Aoe S, et al: Role of the toll-like receptor 4/NF-kappaB pathway in saturated fatty acid-induced inflammatory changes in the interaction between adipocytes and macrophages. Arterioscler Thromb Vasc Biol. 27:84–91. 2007. View Article : Google Scholar : PubMed/NCBI

15 

Yamashita A, Soga Y, Iwamoto Y, Yoshizawa S, Iwata H, Kokeguchi S, Takashiba S and Nishimura F: Macrophage-adipocyte interaction: Marked interleukin-6 production by lipopolysaccharide. Obesity (Silver Spring). 15:2549–2552. 2007. View Article : Google Scholar : PubMed/NCBI

16 

Lai N, Jayaraman A and Lee K: Enhanced proliferation of human umbilical vein endothelial cells and differentiation of 3T3-L1 adipocytes in coculture. Tissue Eng Part A. 15:1053–1061. 2009. View Article : Google Scholar : PubMed/NCBI

17 

Iwashima Y, Katsuya T, Ishikawa K, Kida I, Ohishi M, Horio T, Ouchi N, Ohashi K, Kihara S, Funahashi T, et al: Association of hypoadiponectinemia with smoking habit in men. Hypertension. 45:1094–1100. 2005. View Article : Google Scholar : PubMed/NCBI

18 

Gao YJ, Holloway AC, Su LY, Takemori K, Lu C and Lee RM: Effects of fetal and neonatal exposure to nicotine on blood pressure and perivascular adipose tissue function in adult life. Eur J Pharmacol. 590:264–268. 2008. View Article : Google Scholar : PubMed/NCBI

19 

Thielen A, Klus H and Müller L: Tobacco smoke: Unraveling a controversial subject. Exp Toxicol Pathol. 60:141–156. 2008. View Article : Google Scholar : PubMed/NCBI

20 

Albaugh G, Kann B, Strande L, Vemulapalli P, Hewitt C and Alexander JB: Nicotine induces endothelial TNF-alpha expression, which mediates growth retardation in vitro. J Surg Res. 99:381–384. 2001. View Article : Google Scholar : PubMed/NCBI

21 

Deng G, Long Y, Yu YR and Li MR: Adiponectin directly improves endothelial dysfunction in obese rats through the AMPK-eNOS pathway. Int J Obes (Lond). 34:165–171. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Wang Y, Wang X, Lau WB, Yuan Y, Booth D, Li JJ, Scalia R, Preston K, Gao E, Koch W and Ma XL: Adiponectin inhibits tumor necrosis factor-alpha-induced vascular inflammatory response via caveolin-mediated ceramidase recruitment and activation. Circ Res. 114:792–805. 2014. View Article : Google Scholar : PubMed/NCBI

23 

Wang Y, Wang L, Ai X, Zhao J, Hao X, Lu Y and Qiao Z: Nicotine could augment adhesion molecule expression in human endothelial cells through macrophages secreting TNF-alpha, IL-1beta. Int Immunopharmacol. 4:1675–1686. 2004. View Article : Google Scholar : PubMed/NCBI

24 

Ueno H, Pradhan S, Schlessel D, Hirasawa H and Sumpio BE: Nicotine enhances human vascular endothelial cell expression of ICAM-1 and VCAM-1 via protein kinase C, p38 mitogen-activated protein kinase, NF-kappaB, and AP-1. Cardiovasc Toxicol. 6:39–50. 2006. View Article : Google Scholar : PubMed/NCBI

25 

Gonçalves RB, Coletta RD, Silvério KG, Benevides L, Casati MZ, da Silva JS and Nociti FH Jr: Impact of smoking on inflammation: Overview of molecular mechanisms. Inflamm Res. 60:409–424. 2011. View Article : Google Scholar : PubMed/NCBI

26 

Tsiara S, Elisaf M and Mikhailidis DP: Influence of smoking on predictors of vascular disease. Angiology. 54:507–530. 2003. View Article : Google Scholar : PubMed/NCBI

27 

Yanbaeva DG, Dentener MA, Creutzberg EC, Wesseling G and Wouters EF: Systemic effects of smoking. Chest. 131:1557–1566. 2007. View Article : Google Scholar : PubMed/NCBI

28 

Ouchi N, Kihara S, Arita Y, Okamoto Y, Maeda K, Kuriyama H, Hotta K, Nishida M, Takahashi M, Muraguchi M, et al: Adiponectin, an adipocyte-derived plasma protein, inhibits endothelial NF-kappaB signaling through a cAMP-dependent pathway. Circulation. 102:1296–1301. 2000. View Article : Google Scholar : PubMed/NCBI

29 

Li FY, Cheng KK, Lam KS, Vanhoutte PM and Xu A: Cross-talk between adipose tissue and vasculature: Role of adiponectin. Acta Physiol (Oxf). 203:167–180. 2011. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Wang CN, Yang GH, Wang ZQ, Liu CW, Li TJ, Lai ZC, Miao SY, Wang LF and Liu B: Role of perivascular adipose tissue in nicotine‑induced endothelial cell inflammatory responses. Mol Med Rep 14: 5713-5718, 2016.
APA
Wang, C., Yang, G., Wang, Z., Liu, C., Li, T., Lai, Z. ... Liu, B. (2016). Role of perivascular adipose tissue in nicotine‑induced endothelial cell inflammatory responses. Molecular Medicine Reports, 14, 5713-5718. https://doi.org/10.3892/mmr.2016.5934
MLA
Wang, C., Yang, G., Wang, Z., Liu, C., Li, T., Lai, Z., Miao, S., Wang, L., Liu, B."Role of perivascular adipose tissue in nicotine‑induced endothelial cell inflammatory responses". Molecular Medicine Reports 14.6 (2016): 5713-5718.
Chicago
Wang, C., Yang, G., Wang, Z., Liu, C., Li, T., Lai, Z., Miao, S., Wang, L., Liu, B."Role of perivascular adipose tissue in nicotine‑induced endothelial cell inflammatory responses". Molecular Medicine Reports 14, no. 6 (2016): 5713-5718. https://doi.org/10.3892/mmr.2016.5934
Copy and paste a formatted citation
x
Spandidos Publications style
Wang CN, Yang GH, Wang ZQ, Liu CW, Li TJ, Lai ZC, Miao SY, Wang LF and Liu B: Role of perivascular adipose tissue in nicotine‑induced endothelial cell inflammatory responses. Mol Med Rep 14: 5713-5718, 2016.
APA
Wang, C., Yang, G., Wang, Z., Liu, C., Li, T., Lai, Z. ... Liu, B. (2016). Role of perivascular adipose tissue in nicotine‑induced endothelial cell inflammatory responses. Molecular Medicine Reports, 14, 5713-5718. https://doi.org/10.3892/mmr.2016.5934
MLA
Wang, C., Yang, G., Wang, Z., Liu, C., Li, T., Lai, Z., Miao, S., Wang, L., Liu, B."Role of perivascular adipose tissue in nicotine‑induced endothelial cell inflammatory responses". Molecular Medicine Reports 14.6 (2016): 5713-5718.
Chicago
Wang, C., Yang, G., Wang, Z., Liu, C., Li, T., Lai, Z., Miao, S., Wang, L., Liu, B."Role of perivascular adipose tissue in nicotine‑induced endothelial cell inflammatory responses". Molecular Medicine Reports 14, no. 6 (2016): 5713-5718. https://doi.org/10.3892/mmr.2016.5934
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team