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Neuroprotective effects of polydatin against mitochondrial-dependent apoptosis in the rat cerebral cortex following ischemia/reperfusion injury

  • Authors:
    • Youguang Gao
    • Ting Chen
    • Xianghui Lei
    • Yunfeng Li
    • Xingui Dai
    • Yuanyuan Cao
    • Qionglei Ding
    • Xiabao Lei
    • Tao Li
    • Xianzhong Lin
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian 350005, P.R. China, Department of Pathology, The First People's Hospital of Chenzhou, Institute of Translation Medicine, University of South China, Chenzhou, Hunan 423000, P.R. China, Department of Critical Care Medicine, The First People's Hospital of Chenzhou, Institute of Translation Medicine, University of South China, Chenzhou, Hunan 423000, P.R. China
    Copyright: © Gao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5481-5488
    |
    Published online on: November 11, 2016
       https://doi.org/10.3892/mmr.2016.5936
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Abstract

The neuroprotective effect of polydatin (PD) against hemorrhagic shock-induced mitochondrial injury has been described previously, and mitochondrial dysfunction and apoptosis were reportedly involved in ischemic stroke. In the present study the neuroprotective effect of PD in preventing apoptosis was evaluated following induction of focal cerebral ischemia by middle cerebral artery occlusion (MCAO) in rats. PD (30 mg/kg) was administered by caudal vein injection 10 min prior to ischemia/reperfusion (I/R) injury. 24 h following I/R injury, ameliorated modified neurological severity scores (mNSS) and reduced infarct volume were observed in the PD treated group. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining and Annexin V/propidium iodide assays demonstrated the anti-apoptotic effect of PD in the ischemic cortex. In addition, PD improved I/R injury‑induced mitochondrial dysfunction, reflected by morphological observations and measurements of mitochondrial membrane potential and intracellular ATP measurement. Western blot analysis revealed an increase in B‑cell lymphoma 2 apoptosis regulator (Bcl-2) expression, and a decrease in Bcl‑2‑associated protein X apoptosis regulator expression in the PD group in comparison with the vehicle treated group. PD treatment also prevented the release of cytochrome c from mitochondria into the cytoplasm, and blunted the activities of caspase‑9 and caspase‑3. Furthermore, PD treatment decreased the levels of reactive oxygen species in neurons isolated from the ischemic cortex. The findings of this study, therefore, suggest that PD has a dual effect, ameliorating both oxidative stress and mitochondria‑dependent apoptosis, making it a promising new therapy for the treatment of ischemic stroke.
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Copy and paste a formatted citation
Spandidos Publications style
Gao Y, Chen T, Lei X, Li Y, Dai X, Cao Y, Ding Q, Lei X, Li T, Lin X, Lin X, et al: Neuroprotective effects of polydatin against mitochondrial-dependent apoptosis in the rat cerebral cortex following ischemia/reperfusion injury. Mol Med Rep 14: 5481-5488, 2016.
APA
Gao, Y., Chen, T., Lei, X., Li, Y., Dai, X., Cao, Y. ... Lin, X. (2016). Neuroprotective effects of polydatin against mitochondrial-dependent apoptosis in the rat cerebral cortex following ischemia/reperfusion injury. Molecular Medicine Reports, 14, 5481-5488. https://doi.org/10.3892/mmr.2016.5936
MLA
Gao, Y., Chen, T., Lei, X., Li, Y., Dai, X., Cao, Y., Ding, Q., Lei, X., Li, T., Lin, X."Neuroprotective effects of polydatin against mitochondrial-dependent apoptosis in the rat cerebral cortex following ischemia/reperfusion injury". Molecular Medicine Reports 14.6 (2016): 5481-5488.
Chicago
Gao, Y., Chen, T., Lei, X., Li, Y., Dai, X., Cao, Y., Ding, Q., Lei, X., Li, T., Lin, X."Neuroprotective effects of polydatin against mitochondrial-dependent apoptosis in the rat cerebral cortex following ischemia/reperfusion injury". Molecular Medicine Reports 14, no. 6 (2016): 5481-5488. https://doi.org/10.3892/mmr.2016.5936
Copy and paste a formatted citation
x
Spandidos Publications style
Gao Y, Chen T, Lei X, Li Y, Dai X, Cao Y, Ding Q, Lei X, Li T, Lin X, Lin X, et al: Neuroprotective effects of polydatin against mitochondrial-dependent apoptosis in the rat cerebral cortex following ischemia/reperfusion injury. Mol Med Rep 14: 5481-5488, 2016.
APA
Gao, Y., Chen, T., Lei, X., Li, Y., Dai, X., Cao, Y. ... Lin, X. (2016). Neuroprotective effects of polydatin against mitochondrial-dependent apoptosis in the rat cerebral cortex following ischemia/reperfusion injury. Molecular Medicine Reports, 14, 5481-5488. https://doi.org/10.3892/mmr.2016.5936
MLA
Gao, Y., Chen, T., Lei, X., Li, Y., Dai, X., Cao, Y., Ding, Q., Lei, X., Li, T., Lin, X."Neuroprotective effects of polydatin against mitochondrial-dependent apoptosis in the rat cerebral cortex following ischemia/reperfusion injury". Molecular Medicine Reports 14.6 (2016): 5481-5488.
Chicago
Gao, Y., Chen, T., Lei, X., Li, Y., Dai, X., Cao, Y., Ding, Q., Lei, X., Li, T., Lin, X."Neuroprotective effects of polydatin against mitochondrial-dependent apoptosis in the rat cerebral cortex following ischemia/reperfusion injury". Molecular Medicine Reports 14, no. 6 (2016): 5481-5488. https://doi.org/10.3892/mmr.2016.5936
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