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Article

Hsa‑miR‑371‑5p inhibits human mesangial cell proliferation and promotes apoptosis in lupus nephritis by directly targeting hypoxia‑inducible factor 1α

  • Authors:
    • Feifei Yao
    • Liqiu Sun
    • Wei Fang
    • Huamin Wang
    • Dongsheng Yao
    • Rui Cui
    • Jia Xu
    • Li Wang
    • Xiumei Wang
  • View Affiliations / Copyright

    Affiliations: Department of Nephrology, The Fourth Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China
  • Pages: 5693-5698
    |
    Published online on: November 14, 2016
       https://doi.org/10.3892/mmr.2016.5939
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Abstract

MicroRNAs (miRNAs/miR) have emerged as a novel class of gene expression modulators in kidney disease. Lupus nephritis (LN) is the predominant cause of morbidity and mortality in patients with systemic lupus erythematosus (SLE). Hsa‑miR‑371‑5p has previously been reported to be dysregulated in LN using a miRNA microarray analysis. The present study aimed to determine the function and molecular mechanisms of hsa‑miR‑371‑5p in human mesangial cells of LN. Quantitative polymerase chain reaction (qPCR) was used to detect hsa‑miR‑371‑5p expression in LN tissues. Furthermore, the MTT assay and flow cytometric analyses were performed to analyze the effects of hsa‑miR‑371‑5p on mesangial cell proliferation and apoptosis. Bioinformatics analysis, luciferase reporter assay, qPCR and western blotting were also conducted to predict and confirm the target gene of hsa‑miR‑371‑5p in mesangial cells. The results demonstrated that hsa‑miR‑371‑5p expression was markedly downregulated in LN renal tissues compared with in normal kidney tissues. Restoration of hsa‑miR‑371‑5p expression using synthetic hsa‑miR‑371‑5p mimics was able to significantly inhibit mesangial cell proliferation and induce apoptosis. In addition, mechanistic exploration demonstrated that hypoxia‑inducible factor 1α (HIF‑1α) was a direct target gene of hsa‑miR‑371‑5p in mesangial cells. In conclusion, these results suggested that hsa‑miR‑371‑5p is downregulated in LN, and overexpression of hsa‑miR‑371‑5p may inhibit mesangial cell proliferation and promote apoptosis by directly targeting HIF‑1α.
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1 

Pan Y and Sawalha AH: Epigenetic regulation and the pathogenesis of systemic lupus erythematosus. Transl Res. 153:4–10. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Yung S and Chan TM: Autoantibodies and resident renal cells in the pathogenesis of lupus nephritis: Getting to know the unknown. Clin Dev Immunol. 2012:1393652012. View Article : Google Scholar : PubMed/NCBI

3 

Lewis EJ and Schwartz MM: Pathology of lupus nephritis. Lupus. 14:31–38. 2005. View Article : Google Scholar : PubMed/NCBI

4 

Aran AA and Putterman C: Treatment of lupus nephritis: Facing the era of immunotherapy. Panminerva Med. 50:235–245. 2008.PubMed/NCBI

5 

He L and Hannon GJ: MicroRNAs: Small RNAs with a big role in gene regulation. Nat Rev Genet. 5:522–531. 2004. View Article : Google Scholar : PubMed/NCBI

6 

Bartel DP: MicroRNAs: Genomics, biogenesis, mechanism and function. Cell. 116:281–297. 2004. View Article : Google Scholar : PubMed/NCBI

7 

Davis-Dusenbery BN and Hata A: Mechanisms of control of microRNA biogenesis. J Biochem. 148:381–392. 2010.PubMed/NCBI

8 

Kim VN, Han J and Siomi MC: Biogenesis of small RNAs in animals. Nat Rev Mol Cell Biol. 10:126–139. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Mendell JT: MicroRNAs: Critical regulators of development, cellular physiology and malignancy. Cell Cycle. 4:1179–1184. 2005. View Article : Google Scholar : PubMed/NCBI

10 

Calin GA and Croce CM: MicroRNA signatures in human cancers. Nat Rev Cancer. 6:857–866. 2006. View Article : Google Scholar : PubMed/NCBI

11 

Dai Y, Sui W, Lan H, Yan Q, Huang H and Huang Y: Comprehensive analysis of microRNA expression patterns in renal biopsies of lupus nephritis patients. Rheumatol Int. 29:749–754. 2009. View Article : Google Scholar : PubMed/NCBI

12 

Denby L, Ramdas V, McBride MW, Wang J, Robinson H, McClure J, Crawford W, Lu R, Hillyard DZ, Khanin R, et al: miR-21 and miR-214 are consistently modulated during renal injury in rodent models. Am J Pathol. 179:661–672. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Te JL, Dozmorov IM, Guthridge JM, Nguyen KL, Cavett JW, Kelly JA, Bruner GR, Harley JB and Ojwang JO: Identification of unique microRNA signature associated with lupus nephritis. PLoS One. 5:e103442010. View Article : Google Scholar : PubMed/NCBI

14 

Wang Y, Yu F, Song D, Wang SX and Zhao MH: Podocyte involvement in lupus nephritis based on the 2003 ISN/RPS system: A large cohort study from a single centre. Rheumatology (Oxford). 53:1235–1244. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Arocho A, Chen B, Ladanyi M and Pan Q: Validation of the 2-DeltaDeltaCt calculation as an alternate method of data analysis for quantitative PCR of BCR-ABL P210 transcripts. Diagn Mol Pathol. 15:56–61. 2006. View Article : Google Scholar : PubMed/NCBI

16 

Chafin CB and Reilly CM: MicroRNAs implicated in the immunopathogenesis of lupus nephritis. Clin Dev Immunol. 2013:4302392013. View Article : Google Scholar : PubMed/NCBI

17 

Zhou H, Hasni SA, Perez P, Tandon M, Jang SI, Zheng C, Kopp JB, Austin H III, Balow JE, Alevizos I and Illei GG: miR-150 promotes renal fibrosis in lupus nephritis by downregulating SOCS1. J Am Soc Nephrol. 24:1073–1087. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Akkina S and Becker BN: MicroRNAs in kidney function and disease. Transl Res. 157:236–240. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Chen YQ, Wang XX, Yao XM, Zhang DL, Yang XF, Tian SF and Wang NS: Abated microRNA-195 expression protected mesangial cells from apoptosis in early diabetic renal injury in mice. J Nephrol. 25:566–576. 2012. View Article : Google Scholar : PubMed/NCBI

20 

Trionfini P, Benigni A and Remuzzi G: MicroRNAs in kidney physiology and disease. Nat Rev Nephrol. 11:23–33. 2015. View Article : Google Scholar : PubMed/NCBI

21 

Costa-Reis P, Russo PA, Zhang Z, Colonna L, Maurer K, Gallucci S, Schulz SW, Kiani AN, Petri M and Sullivan KE: The role of microRNAs and human epidermal growth factor receptor 2 in proliferative lupus nephritis. Arthritis Rheumatol. 67:2415–2426. 2015. View Article : Google Scholar : PubMed/NCBI

22 

Liu W, Liu H, Li T and Xu G: MicroRNA-490-43p regulates cell proliferation and apoptosis by targeting HMGA2 in osteosarcoma. FEBS Lett. 589:3148–3153. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Luo H, Guo W, Wang F, You Y, Wang J, Chen X, Wang J, Wang Y, Du Y, Chen X, et al: miR-1291 targets mucin 1 inhibiting cell proliferation and invasion to promote cell apoptosis in esophageal squamous cell carcinoma. Oncol Rep. 34:2665–2673. 2015.PubMed/NCBI

24 

Zhang S, Zhang C, Liu W, Zheng W, Zhang Y, Wang S, Huang D, Liu X and Bai Z: MicroRNA-24 upregulation inhibits proliferation, metastasis and induces apoptosis in bladder cancer cells by targeting CARMA3. Int J Oncol. 47:1351–1360. 2015.PubMed/NCBI

25 

Mao S and Huang S: The signaling pathway of hypoxia inducible factor and its role in renal diseases. J Recept Signal Transduct Res. 33:344–348. 2013. View Article : Google Scholar : PubMed/NCBI

26 

Fähling M, Mathia S, Paliege A, Koesters R, Mrowka R, Peters H, Persson PB, Neumayer HH, Bachmann S and Rosenberger C: Tubular von hippel-lindau knockout protects against rhabdomyolysis-induced AKI. J Am Soc Nephrol. 24:1806–1819. 2013. View Article : Google Scholar : PubMed/NCBI

27 

Haase VH: Hypoxia-inducible factor signaling in the development of kidney fibrosis. Fibrogenesis Tissue Repair. 5:(Suppl 1). S162012.PubMed/NCBI

28 

Kimura K, Iwano M, Higgins DF, Yamaguchi Y, Nakatani K, Harada K, Kubo A, Akai Y, Rankin EB, Neilson EG, et al: Stable expression of HIF-1alpha in tubular epithelial cells promotes interstitial fibrosis. Am J Physiol Renal Physiol. 295:F1023–F1029. 2008. View Article : Google Scholar : PubMed/NCBI

29 

Deng W, Ren Y, Feng X, Yao G, Chen W, Sun Y, Wang H, Gao X and Sun L: Hypoxia inducible factor-1 alpha promotes mesangial cell proliferation in lupus nephritis. Am J Nephrol. 40:507–515. 2014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yao F, Sun L, Fang W, Wang H, Yao D, Cui R, Xu J, Wang L and Wang X: Hsa‑miR‑371‑5p inhibits human mesangial cell proliferation and promotes apoptosis in lupus nephritis by directly targeting hypoxia‑inducible factor 1α. Mol Med Rep 14: 5693-5698, 2016.
APA
Yao, F., Sun, L., Fang, W., Wang, H., Yao, D., Cui, R. ... Wang, X. (2016). Hsa‑miR‑371‑5p inhibits human mesangial cell proliferation and promotes apoptosis in lupus nephritis by directly targeting hypoxia‑inducible factor 1α. Molecular Medicine Reports, 14, 5693-5698. https://doi.org/10.3892/mmr.2016.5939
MLA
Yao, F., Sun, L., Fang, W., Wang, H., Yao, D., Cui, R., Xu, J., Wang, L., Wang, X."Hsa‑miR‑371‑5p inhibits human mesangial cell proliferation and promotes apoptosis in lupus nephritis by directly targeting hypoxia‑inducible factor 1α". Molecular Medicine Reports 14.6 (2016): 5693-5698.
Chicago
Yao, F., Sun, L., Fang, W., Wang, H., Yao, D., Cui, R., Xu, J., Wang, L., Wang, X."Hsa‑miR‑371‑5p inhibits human mesangial cell proliferation and promotes apoptosis in lupus nephritis by directly targeting hypoxia‑inducible factor 1α". Molecular Medicine Reports 14, no. 6 (2016): 5693-5698. https://doi.org/10.3892/mmr.2016.5939
Copy and paste a formatted citation
x
Spandidos Publications style
Yao F, Sun L, Fang W, Wang H, Yao D, Cui R, Xu J, Wang L and Wang X: Hsa‑miR‑371‑5p inhibits human mesangial cell proliferation and promotes apoptosis in lupus nephritis by directly targeting hypoxia‑inducible factor 1α. Mol Med Rep 14: 5693-5698, 2016.
APA
Yao, F., Sun, L., Fang, W., Wang, H., Yao, D., Cui, R. ... Wang, X. (2016). Hsa‑miR‑371‑5p inhibits human mesangial cell proliferation and promotes apoptosis in lupus nephritis by directly targeting hypoxia‑inducible factor 1α. Molecular Medicine Reports, 14, 5693-5698. https://doi.org/10.3892/mmr.2016.5939
MLA
Yao, F., Sun, L., Fang, W., Wang, H., Yao, D., Cui, R., Xu, J., Wang, L., Wang, X."Hsa‑miR‑371‑5p inhibits human mesangial cell proliferation and promotes apoptosis in lupus nephritis by directly targeting hypoxia‑inducible factor 1α". Molecular Medicine Reports 14.6 (2016): 5693-5698.
Chicago
Yao, F., Sun, L., Fang, W., Wang, H., Yao, D., Cui, R., Xu, J., Wang, L., Wang, X."Hsa‑miR‑371‑5p inhibits human mesangial cell proliferation and promotes apoptosis in lupus nephritis by directly targeting hypoxia‑inducible factor 1α". Molecular Medicine Reports 14, no. 6 (2016): 5693-5698. https://doi.org/10.3892/mmr.2016.5939
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