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Article

Obesity‑associated miR‑148a is regulated by cytokines and adipokines via a transcriptional mechanism

  • Authors:
    • Chunmei Shi
    • Lingxia Pang
    • Chenbo Ji
    • Jiaqin Wang
    • Ning Lin
    • Jiantao Chen
    • Ling Chen
    • Lei Yang
    • Fangyan Huang
    • Yahui Zhou
    • Xirong Guo
    • Hui Liang
    • Min Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Children Health Care, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China, Department of Pediatrics, The Third Affiliated Hospital of Xinxiang Medical College, Xinxiang, Henan 453003, P.R. China, Department of Clinical Laboratory, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, Jiangsu 223300, P.R. China, Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China
  • Pages: 5707-5712
    |
    Published online on: November 14, 2016
       https://doi.org/10.3892/mmr.2016.5940
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Abstract

Our previous study revealed that miR‑148a, a cyclic adenosine monophosphate‑response element binding protein‑modulated microRNA that promotes adipocyte differentiation by inhibiting Wnt1, is a biomarker of obesity in human subjects and a mouse model. The present study investigated the expression of miR‑148a in human adipose tissue‑derived mesenchymal stem cells (hMSCs‑Ad) in response to inflammatory cytokines and adipokines to clarify its underlying mechanism. miR‑148a expression was detected using reverse transcription‑quantitative polymerase chain reaction analysis and its promoter activity was detected with a luciferase assay. miR‑148a expression levels decreased when differentiated hMSCs‑Ad were exposed to inflammatory cytokines or adipokines, which suggested that miR‑148a may be important in adipocyte metabolism and inflammation. Furthermore, the promoter activity of miR‑148a decreased following treatment of cells with inflammatory cytokines or adipokines. The results of the present study indicated a novel role of miR‑148a in adipocyte inflammation; therefore, miR‑148a may be involved in obesity complications via its own underlying transcriptional mechanism.
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Copy and paste a formatted citation
Spandidos Publications style
Shi C, Pang L, Ji C, Wang J, Lin N, Chen J, Chen L, Yang L, Huang F, Zhou Y, Zhou Y, et al: Obesity‑associated miR‑148a is regulated by cytokines and adipokines via a transcriptional mechanism. Mol Med Rep 14: 5707-5712, 2016.
APA
Shi, C., Pang, L., Ji, C., Wang, J., Lin, N., Chen, J. ... Zhang, M. (2016). Obesity‑associated miR‑148a is regulated by cytokines and adipokines via a transcriptional mechanism. Molecular Medicine Reports, 14, 5707-5712. https://doi.org/10.3892/mmr.2016.5940
MLA
Shi, C., Pang, L., Ji, C., Wang, J., Lin, N., Chen, J., Chen, L., Yang, L., Huang, F., Zhou, Y., Guo, X., Liang, H., Zhang, M."Obesity‑associated miR‑148a is regulated by cytokines and adipokines via a transcriptional mechanism". Molecular Medicine Reports 14.6 (2016): 5707-5712.
Chicago
Shi, C., Pang, L., Ji, C., Wang, J., Lin, N., Chen, J., Chen, L., Yang, L., Huang, F., Zhou, Y., Guo, X., Liang, H., Zhang, M."Obesity‑associated miR‑148a is regulated by cytokines and adipokines via a transcriptional mechanism". Molecular Medicine Reports 14, no. 6 (2016): 5707-5712. https://doi.org/10.3892/mmr.2016.5940
Copy and paste a formatted citation
x
Spandidos Publications style
Shi C, Pang L, Ji C, Wang J, Lin N, Chen J, Chen L, Yang L, Huang F, Zhou Y, Zhou Y, et al: Obesity‑associated miR‑148a is regulated by cytokines and adipokines via a transcriptional mechanism. Mol Med Rep 14: 5707-5712, 2016.
APA
Shi, C., Pang, L., Ji, C., Wang, J., Lin, N., Chen, J. ... Zhang, M. (2016). Obesity‑associated miR‑148a is regulated by cytokines and adipokines via a transcriptional mechanism. Molecular Medicine Reports, 14, 5707-5712. https://doi.org/10.3892/mmr.2016.5940
MLA
Shi, C., Pang, L., Ji, C., Wang, J., Lin, N., Chen, J., Chen, L., Yang, L., Huang, F., Zhou, Y., Guo, X., Liang, H., Zhang, M."Obesity‑associated miR‑148a is regulated by cytokines and adipokines via a transcriptional mechanism". Molecular Medicine Reports 14.6 (2016): 5707-5712.
Chicago
Shi, C., Pang, L., Ji, C., Wang, J., Lin, N., Chen, J., Chen, L., Yang, L., Huang, F., Zhou, Y., Guo, X., Liang, H., Zhang, M."Obesity‑associated miR‑148a is regulated by cytokines and adipokines via a transcriptional mechanism". Molecular Medicine Reports 14, no. 6 (2016): 5707-5712. https://doi.org/10.3892/mmr.2016.5940
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