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Article Open Access

Regulatory T cells exhibit neuroprotective effect in a mouse model of traumatic brain injury

Retraction in: /10.3892/mmr.2017.6299
  • Authors:
    • Yunhu Yu
    • Fang Cao
    • Qishan Ran
    • Xiaochuan Sun
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China, Department of Cerebrovascular Disease, The First Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou 563000, P.R. China, Department of Neurosurgery, The Third Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou 563000, P.R. China
    Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5556-5566
    |
    Published online on: November 18, 2016
       https://doi.org/10.3892/mmr.2016.5954
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Abstract

Traumatic brain injury (TBI) is a major health and socioeconomic problem as it is associated with high rates of mortality and morbidity worldwide. Regulatory T cells (Tregs) have been reported to reduce inflammatory response in several diseases, including myasthenia gravis, viral myocarditis and cerebral infarction. The present study investigated the role of Tregs in mediating neuro‑protective effects in a mouse model of TBI. Initially, Treg levels were determined, and compared between the controlled cortical impact (CCI) model for moderate TBI and the sham group, by using flow cytometry and ELISA. Afterwards, the number of Tregs was upregulated (by injection) and downregulated (by depletion), respectively, to elucidate the effect of Tregs in the presence of an inflammatory reaction and a deficient neurological function and consequently, in the prognosis of TBI in the mouse. The expression of pro‑inflammatory cytokines [tumor necrosis factor (TNF)‑α, interleukin (IL)‑1β, IL‑6)] and anti‑inflammatory cytokines [IL‑10, transforming growth factor (TGF)‑β] in blood and brain tissues was also measured in the five groups: Μice receiving a saline injection, mice experiencing Treg depletion, small‑dose (SD Tregs, 1.25x105), and mice receiving different doses of Tregs: Moderate‑dose (MD Tregs, 2.5x105) and large‑dose (LD Tregs, 5x105), using ELISA and PCR. Co‑cultures of Tregs and microglia were performed to evaluate the expression of pro‑inflammatory cytokines and observe the interaction between the two types of cells. The regulation patterns in JNK‑NF‑κB pathway by Tregs were also evaluated by western blot analysis. Treg levels were significantly reduced in TBI mouse group on the 3rd day after TBI (P<0.05). In the mouse model of TBI, the expression of pro‑inflammatory cytokines (TNF‑α, IL‑1β, IL‑6) was enhanced, while the expression of anti‑inflammatory cytokines (IL‑10, TGF‑β) was reduced (P<0.05). Tregs exhibited a suppressive effect on inflammatory reactions. In the MD group, the activation of microglia cells was markedly inhibited, compared to the activation in SD and LD groups. The expression of ERK1/2, JNK1/2/3 and NK‑κB was significantly downregulated in the MD group. The results indicated that Tregs exhibited significant neuro‑protective effects, suppressing pro‑inflammatory responses and promoting tissue repair after TBI injury in the mouse, specifically by deactivating the JNK‑NF‑κB pathway. The results of the study show that Tregs potentially participates in neuro‑therapeutic approaches for TBI.
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Copy and paste a formatted citation
Spandidos Publications style
Yu Y, Cao F, Ran Q and Sun X: Regulatory T cells exhibit neuroprotective effect in a mouse model of traumatic brain injury Retraction in /10.3892/mmr.2017.6299. Mol Med Rep 14: 5556-5566, 2016.
APA
Yu, Y., Cao, F., Ran, Q., & Sun, X. (2016). Regulatory T cells exhibit neuroprotective effect in a mouse model of traumatic brain injury Retraction in /10.3892/mmr.2017.6299. Molecular Medicine Reports, 14, 5556-5566. https://doi.org/10.3892/mmr.2016.5954
MLA
Yu, Y., Cao, F., Ran, Q., Sun, X."Regulatory T cells exhibit neuroprotective effect in a mouse model of traumatic brain injury Retraction in /10.3892/mmr.2017.6299". Molecular Medicine Reports 14.6 (2016): 5556-5566.
Chicago
Yu, Y., Cao, F., Ran, Q., Sun, X."Regulatory T cells exhibit neuroprotective effect in a mouse model of traumatic brain injury Retraction in /10.3892/mmr.2017.6299". Molecular Medicine Reports 14, no. 6 (2016): 5556-5566. https://doi.org/10.3892/mmr.2016.5954
Copy and paste a formatted citation
x
Spandidos Publications style
Yu Y, Cao F, Ran Q and Sun X: Regulatory T cells exhibit neuroprotective effect in a mouse model of traumatic brain injury Retraction in /10.3892/mmr.2017.6299. Mol Med Rep 14: 5556-5566, 2016.
APA
Yu, Y., Cao, F., Ran, Q., & Sun, X. (2016). Regulatory T cells exhibit neuroprotective effect in a mouse model of traumatic brain injury Retraction in /10.3892/mmr.2017.6299. Molecular Medicine Reports, 14, 5556-5566. https://doi.org/10.3892/mmr.2016.5954
MLA
Yu, Y., Cao, F., Ran, Q., Sun, X."Regulatory T cells exhibit neuroprotective effect in a mouse model of traumatic brain injury Retraction in /10.3892/mmr.2017.6299". Molecular Medicine Reports 14.6 (2016): 5556-5566.
Chicago
Yu, Y., Cao, F., Ran, Q., Sun, X."Regulatory T cells exhibit neuroprotective effect in a mouse model of traumatic brain injury Retraction in /10.3892/mmr.2017.6299". Molecular Medicine Reports 14, no. 6 (2016): 5556-5566. https://doi.org/10.3892/mmr.2016.5954
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