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Alteration of histone H3 lysine 9 dimethylation in peripheral white blood cells of septic patients with trauma and cancer

  • Authors:
    • Linghui Jiang
    • Yiqin Wang
    • Duming Zhu
    • Zhanggang Xue
    • Hailei Mao
  • View Affiliations / Copyright

    Affiliations: Departments of Anesthesiology and Critical Care Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China, State Key Laboratory of Molecular Biology and Shanghai Key Laboratory of Molecular Andrology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, P.R. China
    Copyright: © Jiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5467-5474
    |
    Published online on: November 22, 2016
       https://doi.org/10.3892/mmr.2016.5958
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Abstract

The present study aimed to investigate the clinical significance of histone methylation in sepsis. A total of 43 blood samples from trauma and esophageal cancer patients with or without sepsis were collected. Immunofluorescence staining of isolated peripheral white blood cells (WBCs) was conducted. Co‑stained 293T cells served as a reference, to allow the levels of histone methylation in different types of WBCs from patients to be determined. Immunostaining analyses revealed different levels of histone 3 lysine 9 dimethylation (H3K9me2) in neutrophils (Neu), lymphocytes (Lym), and monocytes (Mon) from trauma patients. Compared with trauma patients, the levels of H3K9me2 were elevated in the three types of WBCs from cancer patients. When combined with sepsis, trauma patients demonstrated increased H3K9me2 levels in Neu (P=0.0005) and Mon (P=0.0002), whereas cancer patients had a significant decrease of H3K9me2 levels in the three types of WBCs (Neu, P=0.0003; Lym, P=0.007; Mon, P=0.007). The H3K9me2 alterations in patients with trauma and cancer were different with the occurrence of sepsis. A larger cohort study is warranted to explore the diagnostic significance and prognostic implications of altered histone methylation in septic patients.
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Copy and paste a formatted citation
Spandidos Publications style
Jiang L, Wang Y, Zhu D, Xue Z and Mao H: Alteration of histone H3 lysine 9 dimethylation in peripheral white blood cells of septic patients with trauma and cancer. Mol Med Rep 14: 5467-5474, 2016.
APA
Jiang, L., Wang, Y., Zhu, D., Xue, Z., & Mao, H. (2016). Alteration of histone H3 lysine 9 dimethylation in peripheral white blood cells of septic patients with trauma and cancer. Molecular Medicine Reports, 14, 5467-5474. https://doi.org/10.3892/mmr.2016.5958
MLA
Jiang, L., Wang, Y., Zhu, D., Xue, Z., Mao, H."Alteration of histone H3 lysine 9 dimethylation in peripheral white blood cells of septic patients with trauma and cancer". Molecular Medicine Reports 14.6 (2016): 5467-5474.
Chicago
Jiang, L., Wang, Y., Zhu, D., Xue, Z., Mao, H."Alteration of histone H3 lysine 9 dimethylation in peripheral white blood cells of septic patients with trauma and cancer". Molecular Medicine Reports 14, no. 6 (2016): 5467-5474. https://doi.org/10.3892/mmr.2016.5958
Copy and paste a formatted citation
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Spandidos Publications style
Jiang L, Wang Y, Zhu D, Xue Z and Mao H: Alteration of histone H3 lysine 9 dimethylation in peripheral white blood cells of septic patients with trauma and cancer. Mol Med Rep 14: 5467-5474, 2016.
APA
Jiang, L., Wang, Y., Zhu, D., Xue, Z., & Mao, H. (2016). Alteration of histone H3 lysine 9 dimethylation in peripheral white blood cells of septic patients with trauma and cancer. Molecular Medicine Reports, 14, 5467-5474. https://doi.org/10.3892/mmr.2016.5958
MLA
Jiang, L., Wang, Y., Zhu, D., Xue, Z., Mao, H."Alteration of histone H3 lysine 9 dimethylation in peripheral white blood cells of septic patients with trauma and cancer". Molecular Medicine Reports 14.6 (2016): 5467-5474.
Chicago
Jiang, L., Wang, Y., Zhu, D., Xue, Z., Mao, H."Alteration of histone H3 lysine 9 dimethylation in peripheral white blood cells of septic patients with trauma and cancer". Molecular Medicine Reports 14, no. 6 (2016): 5467-5474. https://doi.org/10.3892/mmr.2016.5958
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