Open Access

Anti‑proliferative activity of epigallocatechin‑3‑gallate and silibinin on soft tissue sarcoma cells

  • Authors:
    • Kamran Harati
    • Björn Behr
    • Christoph Wallner
    • Adrien Daigeler
    • Tobias Hirsch
    • Frank Jacobsen
    • Marcus Renner
    • Ali Harati
    • Marcus Lehnhardt
    • Mustafa Becerikli
  • View Affiliations

  • Published online on: November 28, 2016     https://doi.org/10.3892/mmr.2016.5969
  • Pages: 103-110
  • Copyright: © Harati et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Disseminated soft tissue sarcomas (STS) present a therapeutic dilemma. The first-line cytostatic doxorubicin demonstrates a response rate of 30% and is not suitable for elderly patients with underlying cardiac disease, due to its cardiotoxicity. Well‑tolerated alternative treatment options, particularly in palliative situations, are rare. Therefore, the present study assessed the anti‑proliferative effects of the natural compounds epigallocatechin-3-gallate (EGCG), silibinin and noscapine on STS cells. A total of eight different human STS cell lines were used in the study: Fibrosarcoma (HT1080), liposarcoma (SW872, T778 and MLS‑402), synovial sarcoma (SW982, SYO1 and 1273) and pleomorphic sarcoma (U2197). Cell proliferation and viability were analysed by 5‑bromo-2'-deoxyuridine and MTT assays and real‑time cell analysis (RTCA). RTCA indicated that noscapine did not exhibit any inhibitory effects. By contrast, EGCG decreased proliferation and viability of all cell lines except for the 1273 synovial sarcoma cell line. Silibinin exhibited anti‑proliferative effects on all synovial sarcoma, liposarcoma and fibrosarcoma cell lines. Liposarcoma cell lines responded particularly well to EGCG while synovial sarcoma cell lines were more sensitive to silibinin. In conclusion, the green tea polyphenol EGCG and the natural flavonoid silibinin from milk thistle suppressed the proliferation and viability of liposarcoma, synovial sarcoma and fibrosarcoma cells. These compounds are therefore potential candidates as mild therapeutic options for patients that are not suitable for doxorubicin‑based chemotherapy and require palliative treatment. The findings from the present study provide evidence to support in vivo trials assessing the effect of these natural compounds on solid sarcomas.
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January-2017
Volume 15 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Harati K, Behr B, Wallner C, Daigeler A, Hirsch T, Jacobsen F, Renner M, Harati A, Lehnhardt M, Becerikli M, Becerikli M, et al: Anti‑proliferative activity of epigallocatechin‑3‑gallate and silibinin on soft tissue sarcoma cells. Mol Med Rep 15: 103-110, 2017
APA
Harati, K., Behr, B., Wallner, C., Daigeler, A., Hirsch, T., Jacobsen, F. ... Becerikli, M. (2017). Anti‑proliferative activity of epigallocatechin‑3‑gallate and silibinin on soft tissue sarcoma cells. Molecular Medicine Reports, 15, 103-110. https://doi.org/10.3892/mmr.2016.5969
MLA
Harati, K., Behr, B., Wallner, C., Daigeler, A., Hirsch, T., Jacobsen, F., Renner, M., Harati, A., Lehnhardt, M., Becerikli, M."Anti‑proliferative activity of epigallocatechin‑3‑gallate and silibinin on soft tissue sarcoma cells". Molecular Medicine Reports 15.1 (2017): 103-110.
Chicago
Harati, K., Behr, B., Wallner, C., Daigeler, A., Hirsch, T., Jacobsen, F., Renner, M., Harati, A., Lehnhardt, M., Becerikli, M."Anti‑proliferative activity of epigallocatechin‑3‑gallate and silibinin on soft tissue sarcoma cells". Molecular Medicine Reports 15, no. 1 (2017): 103-110. https://doi.org/10.3892/mmr.2016.5969