Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
January-2017 Volume 15 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
January-2017 Volume 15 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Nitrogen gas plasma treatment of bacterial spores induces oxidative stress that damages the genomic DNA

  • Authors:
    • Akikazu Sakudo
    • Yoichi Toyokawa
    • Tetsuji Nakamura
    • Yoshihito Yagyu
    • Yuichiro Imanishi
  • View Affiliations / Copyright

    Affiliations: Laboratory of Biometabolic Chemistry, School of Health Sciences, University of The Ryukyus, Nishihara, Okinawa 903‑0215, Japan, Department of Electrical and Electric Engineering, Sasebo National College of Technology, Nagasaki 857‑1193, Japan, NGK Insulators, Mizuho‑ku, Nagoya, Aichi 467‑8530, Japan
  • Pages: 396-402
    |
    Published online on: November 29, 2016
       https://doi.org/10.3892/mmr.2016.5973
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Gas plasma, produced by a short high‑voltage pulse generated from a static induction thyristor power supply [1.5 kilo pulse/sec (kpps)], was demonstrated to inactivate Geobacillus stearothermophilus spores (decimal reduction time at 15 min, 2.48 min). Quantitative polymerase chain reaction and enzyme‑linked immunosorbent assays further indicated that nitrogen gas plasma treatment for 15 min decreased the level of intact genomic DNA and increased the level of 8-hydroxy-2'-deoxyguanosine, a major product of DNA oxidation. Three potential inactivation factors were generated during operation of the gas plasma instrument: Heat, longwave ultraviolet-A and oxidative stress (production of hydrogen peroxide, nitrite and nitrate). Treatment of the spores with hydrogen peroxide (3x2‑4%) effectively inactivated the bacteria, whereas heat treatment (100˚C), exposure to UV-A (75‑142 mJ/cm2) and 4.92 mM peroxynitrite (•ONOO‑), which is decomposed into nitrite and nitrate, did not. The results of the present study suggest the gas plasma treatment inactivates bacterial spores primarily by generating hydrogen peroxide, which contributes to the oxidation of the host genomic DNA.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

McDonnell GE: Antisepsis, Disinfection, and Sterilization: Types, Action, and Resistance. ASM Press; Washington, D.C: 2007

2 

Sakudo A and Shintani H: Sterilization and disinfection by plasma: Sterilization mechanisms, biological and medical applications. Nova Science Publishers, Inc.; New York: 2011

3 

Laroussi M: Low temperature plasma-based sterilization: Overview and state-of-the-art. Plasma Process Polym. 2:391–400. 2005. View Article : Google Scholar

4 

Moisan M, Barbeau J, Crevier MC, Pelletier J, Philip N and Saoudi B: Plasma sterilization: Methods and mechanisms. Pure Appl Chem. 74:349–358. 2002.

5 

Sakudo A, Shimizu N, Imanishi Y and Ikuta K: N2 gas plasma inactivates influenza virus by inducing changes in viral surface morphology, protein, and genomic RNA. Biomed Res Int. 2013:6942692013.PubMed/NCBI

6 

Shintani H, Shimizu N, Imanishi Y, Sekiya T, Tamazawa K, Taniguchi A and Kido N: Inactivation of microorganisms and endotoxins by low temperature nitrogen gas plasma exposure. Biocontrol Sci. 12:131–143. 2007. View Article : Google Scholar : PubMed/NCBI

7 

Sakudo A, Higa M, Maeda K, Shimizu N, Imanishi Y and Shintani H: Sterilization mechanism of nitrogen gas plasma: Induction of secondary structural change in protein. Microbiol Immunol. 57:536–542. 2013.PubMed/NCBI

8 

Sakudo A, Misawa T, Shimizu N and Imanishi Y: N2 gas plasma inactivates influenza virus mediated by oxidative stress. Front Biosci (Elite Ed). 6:69–79. 2014.PubMed/NCBI

9 

Shintani H, Sakudo A, Burke P and McDonnell G: Gas plasma sterilization of microorganisms and mechanisms of action. Exp Ther Med. 1:731–738. 2010.PubMed/NCBI

10 

Maeda K, Toyokawa Y, Shimizu N, Imanishi Y and Sakudo A: Inactivation of Salmonella by nitrogen gas plasma generated by a static induction thyristor as a pulsed power supply. Food Control. 52:54–59. 2015. View Article : Google Scholar

11 

Sakudo A, Toyokawa Y and Imanishi Y: Nitrogen Gas Plasma Generated by a Static Induction Thyristor as a Pulsed Power Supply Inactivates Adenovirus. PLoS One. 11:e01579222016. View Article : Google Scholar : PubMed/NCBI

12 

Kawamura K, Sakuma A, Nakamura Y, Oguri T, Sato N and Kido N: Evaluation of bactericidal effects of low-temperature nitrogen gas plasma towards application to short-time sterilization. Microbiol Immunol. 56:431–440. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Kuisiene N, Raugalas J, Stuknyte M and Chitavichius D: Identification of the genus Geobacillus using genus-specific primers, based on the 16S-23S Rrna gene internal transcribed spacer. FEMS Microbiol Lett. 277:165–172. 2007. View Article : Google Scholar : PubMed/NCBI

14 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

15 

Halvorson HO and Ziegler NR: Application of Statistics to Problems in Bacteriology: I. A Means of Determining Bacterial Population by the Dilution Method. J Bacteriol. 25:101–121. 1933.PubMed/NCBI

16 

Stumbo CR, Murphy JR and Cochran J: Nature of Thermal Death Time Curves for P.A. 3679 and Clostridium botulinum [1950]. Food Technol. 4:321–326. 1950.

17 

Valavanidis A, Vlachogianni T and Fiotakis C: 8-hydroxy-2′- deoxyguanosine (8-OhdG): A critical biomarker of oxidative stress and carcinogenesis. J Environ Sci Health C Environ Carcinog Ecotoxicol Rev. 27:120–139. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Laroussi M and Leipold F: Evaluation of the Roles of Reactive Species, Heat and UV radiation in the Inactivation of bacterial Cells by Air Plasmas at Atmospheric Pressure. Int J Mass Spectrom. 233:81–86. 2004. View Article : Google Scholar

19 

Laroussi M, Mendis DA and Rosenberg M: Plasma Interaction with Microbes. New J Phys. 5:1–41. 2003.

20 

Shintani H: Application of vapor phase hydrogen peroxide sterilization to endoscope. Biocontrol Sci. 14:39–45. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Shintani H: Simultaneous Achievement of Sterility Assurance Level (SAL) of 10−6 and Material and Functional Compatibility in Gas Plasma Sterilization Running Title: Simultaneous SAL and Compatibility. Pharm Regul Aff. 4:1312015.

22 

Eskenazi S, Bychkowski OE, Smith M and MacMillan JD: Evaluation of glutaraldehyde and hydrogen peroxide for sanitizing packaging materials of medical devices in sterility testing. J Assoc Off Anal Chem. 65:1155–1161. 1982.PubMed/NCBI

23 

Kenar L, Ortatatli M, Yaren H, Karayilanoglu T and Aydogan H: Comparative sporicidal effects of disinfectants after release of a biological agent. Mil Med. 172:616–621. 2007. View Article : Google Scholar : PubMed/NCBI

24 

Setlow B, Yu J, Li YQ and Setlow P: Analysis of the germination kinetics of individual Bacillus subtilis spores treated with hydrogen peroxide or sodium hypochlorite. Lett Appl Microbiol. 57:259–265. 2013.PubMed/NCBI

25 

MACHEREY-NAGEL GmbH & Co. KG, . QUANTOFIX® Active oxygen. simpleftp://ftp.mn-net.com/english/Instruction_leaflets/QUANTOFIX/91349en.pdf#search=%27Quantofix+Active+oxygen%27Accessed 25 August 2015.

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Sakudo A, Toyokawa Y, Nakamura T, Yagyu Y and Imanishi Y: Nitrogen gas plasma treatment of bacterial spores induces oxidative stress that damages the genomic DNA. Mol Med Rep 15: 396-402, 2017.
APA
Sakudo, A., Toyokawa, Y., Nakamura, T., Yagyu, Y., & Imanishi, Y. (2017). Nitrogen gas plasma treatment of bacterial spores induces oxidative stress that damages the genomic DNA. Molecular Medicine Reports, 15, 396-402. https://doi.org/10.3892/mmr.2016.5973
MLA
Sakudo, A., Toyokawa, Y., Nakamura, T., Yagyu, Y., Imanishi, Y."Nitrogen gas plasma treatment of bacterial spores induces oxidative stress that damages the genomic DNA". Molecular Medicine Reports 15.1 (2017): 396-402.
Chicago
Sakudo, A., Toyokawa, Y., Nakamura, T., Yagyu, Y., Imanishi, Y."Nitrogen gas plasma treatment of bacterial spores induces oxidative stress that damages the genomic DNA". Molecular Medicine Reports 15, no. 1 (2017): 396-402. https://doi.org/10.3892/mmr.2016.5973
Copy and paste a formatted citation
x
Spandidos Publications style
Sakudo A, Toyokawa Y, Nakamura T, Yagyu Y and Imanishi Y: Nitrogen gas plasma treatment of bacterial spores induces oxidative stress that damages the genomic DNA. Mol Med Rep 15: 396-402, 2017.
APA
Sakudo, A., Toyokawa, Y., Nakamura, T., Yagyu, Y., & Imanishi, Y. (2017). Nitrogen gas plasma treatment of bacterial spores induces oxidative stress that damages the genomic DNA. Molecular Medicine Reports, 15, 396-402. https://doi.org/10.3892/mmr.2016.5973
MLA
Sakudo, A., Toyokawa, Y., Nakamura, T., Yagyu, Y., Imanishi, Y."Nitrogen gas plasma treatment of bacterial spores induces oxidative stress that damages the genomic DNA". Molecular Medicine Reports 15.1 (2017): 396-402.
Chicago
Sakudo, A., Toyokawa, Y., Nakamura, T., Yagyu, Y., Imanishi, Y."Nitrogen gas plasma treatment of bacterial spores induces oxidative stress that damages the genomic DNA". Molecular Medicine Reports 15, no. 1 (2017): 396-402. https://doi.org/10.3892/mmr.2016.5973
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team