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Article Open Access

Identification of functional pathways associated with the conditional ablation of serum response factor in Dstncorn1 mice

  • Authors:
    • Yanan Huo
    • Xin Xie
    • Bo Jiang
  • View Affiliations / Copyright

    Affiliations: Eye Center, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, P.R. China, Department of Ophthalmology, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China
    Copyright: © Huo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 139-145
    |
    Published online on: December 5, 2016
       https://doi.org/10.3892/mmr.2016.5984
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Abstract

The aim of the present study was to investigate the signaling pathways associated with functional alterations in corneal tissues following the conditional ablation of serum response factor (Srf) in Dstncorn1 mice. The gene expression profiling array GSE49688, which includes 3 samples each from the wild‑type (WT), Dstncorn1 mutant (corn1) and corn1 mice following the conditional ablation of Srf from the corneal epithelium [namely rescued (res)] mouse groups, was downloaded from the Gene Expression Omnibus database. The limma package was used to identify differentially expressed genes (DEGs) among the three mouse groups. DEGs were subsequently analyzed by dynamic comparison, hierarchical clustering and pathway enrichment analysis. Pathway alteration scores were also calculated in order to study the dynamic metergasis of each identified pathway. A total of 788 DEGs were identified between the corn1 and res groups, 1,365 DEGs were identified between the corn1 and WT groups, and 852 DEGs were identified between the res and WT groups. Among these DEGs, 228 genes were differentially expressed across all three groups, and were mainly enriched in signaling pathways involved in the regulation of the actin cytoskeleton, including the cofilin 1 (CFL1), the mitogen‑activated protein kinase (MAPK) signaling pathway and focal adhesion. The dilated cardiomyopathy signaling pathway displayed the highest alteration score, and was enriched with integrin and integrin β‑6 (ITGB6). In conclusion, the actin cytoskeleton regulatory pathway, MAPK and dilated cardiomyopathy signaling pathways, as well as CFL1 and ITGB6 genes, may be regulated by Srf to serve important roles in the progression of corneal disease.
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Copy and paste a formatted citation
Spandidos Publications style
Huo Y, Xie X and Jiang B: Identification of functional pathways associated with the conditional ablation of serum response factor in Dstncorn1 mice. Mol Med Rep 15: 139-145, 2017.
APA
Huo, Y., Xie, X., & Jiang, B. (2017). Identification of functional pathways associated with the conditional ablation of serum response factor in Dstncorn1 mice. Molecular Medicine Reports, 15, 139-145. https://doi.org/10.3892/mmr.2016.5984
MLA
Huo, Y., Xie, X., Jiang, B."Identification of functional pathways associated with the conditional ablation of serum response factor in Dstncorn1 mice". Molecular Medicine Reports 15.1 (2017): 139-145.
Chicago
Huo, Y., Xie, X., Jiang, B."Identification of functional pathways associated with the conditional ablation of serum response factor in Dstncorn1 mice". Molecular Medicine Reports 15, no. 1 (2017): 139-145. https://doi.org/10.3892/mmr.2016.5984
Copy and paste a formatted citation
x
Spandidos Publications style
Huo Y, Xie X and Jiang B: Identification of functional pathways associated with the conditional ablation of serum response factor in Dstncorn1 mice. Mol Med Rep 15: 139-145, 2017.
APA
Huo, Y., Xie, X., & Jiang, B. (2017). Identification of functional pathways associated with the conditional ablation of serum response factor in Dstncorn1 mice. Molecular Medicine Reports, 15, 139-145. https://doi.org/10.3892/mmr.2016.5984
MLA
Huo, Y., Xie, X., Jiang, B."Identification of functional pathways associated with the conditional ablation of serum response factor in Dstncorn1 mice". Molecular Medicine Reports 15.1 (2017): 139-145.
Chicago
Huo, Y., Xie, X., Jiang, B."Identification of functional pathways associated with the conditional ablation of serum response factor in Dstncorn1 mice". Molecular Medicine Reports 15, no. 1 (2017): 139-145. https://doi.org/10.3892/mmr.2016.5984
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