Molecular inhibition mechanisms of cell migration and invasion by coix polysaccharides in A549 NSCLC cells via targeting S100A4

  • Authors:
    • Cheng Luo
    • Xin Wang
    • Can An
    • Chin‑Fa Hwang
    • Wenhua Miao
    • Lu Yang
    • Maonian Xu
    • Aiping Bai
    • Shanggui Deng
  • View Affiliations

  • Published online on: December 5, 2016     https://doi.org/10.3892/mmr.2016.5985
  • Pages: 309-316
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Abstract

S100 calcium binding protein A4 (S100A4) promotes extracellular signal transduction, intercellular adhesion, motility and mobility. Different extracts from Coix lachryma-jobi have been used for the treatment of various types of cancer in Asia. In our previous study, the polysaccharide fraction extact, CP1, induced cell apoptosis of non‑small cell lung cancer cells. In the current study, CP1 inhibited migration and invasion of A549 cells in a scratch wound healing assay and matrigel invasion assay, respectively. Furthermore, reverse transcription‑polymerase chain reaction and western blotting demonstrated that CP1 downregulated the gene and protein expression levels of S100A4. In silico docking analysis demonstrated that polysaccharides may not interfere with dimerization, whereas, the affinity of polysaccharides for an S100A4‑NMIIA pocket was margnially greater than at the dimerization sites. Thus, CP1 inhibited A549 cell migration and invasion potentially via downregulation of S100A4, and may also interact with the binding site of S100A4‑NMIIA, which indicated that CP1 has potential as an alternative cancer chemotherapeutic by targeting S100A4.
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January-2017
Volume 15 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Luo C, Wang X, An C, Hwang CF, Miao W, Yang L, Xu M, Bai A and Deng S: Molecular inhibition mechanisms of cell migration and invasion by coix polysaccharides in A549 NSCLC cells via targeting S100A4. Mol Med Rep 15: 309-316, 2017
APA
Luo, C., Wang, X., An, C., Hwang, C., Miao, W., Yang, L. ... Deng, S. (2017). Molecular inhibition mechanisms of cell migration and invasion by coix polysaccharides in A549 NSCLC cells via targeting S100A4. Molecular Medicine Reports, 15, 309-316. https://doi.org/10.3892/mmr.2016.5985
MLA
Luo, C., Wang, X., An, C., Hwang, C., Miao, W., Yang, L., Xu, M., Bai, A., Deng, S."Molecular inhibition mechanisms of cell migration and invasion by coix polysaccharides in A549 NSCLC cells via targeting S100A4". Molecular Medicine Reports 15.1 (2017): 309-316.
Chicago
Luo, C., Wang, X., An, C., Hwang, C., Miao, W., Yang, L., Xu, M., Bai, A., Deng, S."Molecular inhibition mechanisms of cell migration and invasion by coix polysaccharides in A549 NSCLC cells via targeting S100A4". Molecular Medicine Reports 15, no. 1 (2017): 309-316. https://doi.org/10.3892/mmr.2016.5985