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Article Open Access

5‑bromo‑3‑(3‑hydroxyprop‑1‑ynyl)‑2H‑pyran‑2‑one induces apoptosis in T24 human bladder cancer cells through mitochondria-dependent signaling pathways

Corrigendum in: /10.3892/mmr.2016.6076
  • Authors:
    • Guo‑Qiang Yu
    • Zhong‑Ling Dou
    • Zhao‑Hui Jia
  • View Affiliations / Copyright

    Affiliations: Department of Urological Surgery, The First Affiliated Hospital, College of Clinical Medicine, Henan University of Science and Technology, Luoyang, Henan 471003, P.R. China
    Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 153-159
    |
    Published online on: December 6, 2016
       https://doi.org/10.3892/mmr.2016.5991
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Abstract

The present study was performed to investigate the effect of 5-bromo-3-(3-hydroxyprop-1-ynyl)-2H-pyran-2-one (BHP) on the induction of apoptosis and cell cycle arrest in T24 human bladder carcinoma cells. An MTT assay was used to investigate the inhibition of cell proliferation. Flow cytometry was used to observe alterations in the cell cycle, generation of reactive oxygen species (ROS), alterations in mitochondrial membrane potential (MMP) and induction of apoptosis in the T24 cells following BHP treatment. Western blot analysis was performed for the determination of expression levels of apoptotic proteins, and 4,6‑diamidino‑2‑phenylindole dihydrochloride staining was used to observe apoptosis and DNA damage. The results demonstrated that treatment of the bladder cancer cells with BHP enhanced the activation of caspases and increased the production of ROS. It also caused damage to DNA, reduced MMP, and increased the secretion of endonuclease G and apoptosis‑inducing factor from the mitochondria. The expression levels of cyclin E and cell division cycle 25C were reduced, whereas the expression levels of p21 and phosphorylated p53 were increased in the BHP‑treated cells. In addition, treatment with BHP caused cell cycle arrest at the G0/G1 phase, increased the expression levels of B cell lymphoma‑2 (Bcl‑2)‑associated X protein and poly(ADP‑ribose) polymerase, decreased the expression of Bcl‑2 and ultimately induced apoptosis of the T24 cells. Thus, BHP inhibited the proliferation of bladder cancer cells by inducing cell apoptosis through the mitochondrial pathway.
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1 

Kaufman DS, Shipley WU and Feldman AS: Bladder cancer. Lancet. 374:239–249. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Kwak C, Ku JH, Park JY, Lee E, Lee SE and Lee C: Initial tumor stage and grade as a predictive factor for recurrence in patients with stage T1 grade 3 bladder cancer. J Urol. 171:149–152. 2004. View Article : Google Scholar : PubMed/NCBI

4 

Sternberg CN, Yagoda A, Scher HI, Watson RC, Geller N, Herr HW, Morse MJ, Sogani PC, Vaughan ED, Bander N, et al: Methotrexate, vinblastine, doxorubicin, and cisplatin for advanced transitional cell carcinoma of the urothelium. Efficacy and patterns of response and relapse. Cancer. 64:2448–2458. 1989. View Article : Google Scholar : PubMed/NCBI

5 

Orren DK, Petersen LN and Bohr VA: Persistent DNA damage inhibits S-phase and G2 progression and results in apoptosis. Mol Biol Cell. 8:1129–1142. 1997. View Article : Google Scholar : PubMed/NCBI

6 

Fujimoto K, Hosotani R, Doi R, Wada M, Lee JU, Koshiba T, Miyamoto Y, Tsuji S, Nakajima S and Imamura M: Induction of cell-cycle arrest and apoptosis by a novel retinobenzoic-acid derivative, TAC-101, in human pancreatic-cancer cells. Int J Cancer. 81:637–644. 1999. View Article : Google Scholar : PubMed/NCBI

7 

Gamet-Payrastre L, Li P, Lumeau S, Cassar G, Dupont MA, Chevolleau S, Gasc N, Tulliez J and Tercé F: Sulforaphane, a naturally occurring isothiocyanate, induces cell cycle arrest and apoptosis in HT29 human colon cancer cells. Cancer Res. 60:1426–1433. 2000.PubMed/NCBI

8 

Nakagawa T, Zhu H, Morishima N, Li E, Xu J, Yankner BA and Yuan J: Caspase-l2 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-beta. Nature. 403:98–103. 2000. View Article : Google Scholar : PubMed/NCBI

9 

Srinivasula SM, Hegde R, Saleh A, Datta P, Shiozaki E, Chai J, Lee RA, Robbins PD, Fernandes-Alnemri T, Shi Y and Alnemri ES: A conserved XIAP-interaction motif in caspase-9 and Smac/DIABLO regulates caspase activity and apoptosis. Nature. 409:112–116. 2001.

10 

Degterev A, Boyce M and Yuan J: A decade of caspases. Oncogene. 22:8543–8567. 2003. View Article : Google Scholar : PubMed/NCBI

11 

Ziegler DS and Kung AL: Therapeutic targeting of apoptosis pathways in cancer. Curr Opin Oncol. 20:97–103. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Jensen PR and Fenical W: Strategies for the discovery of secondary metabolites from marine bacteria: Ecological perspectives. Annu Rev Microbiol. 48:559–584. 1994. View Article : Google Scholar : PubMed/NCBI

13 

Faulkner DJ: Marine natural products. Nat Prod Rep. 18:1–49. 2001. View Article : Google Scholar : PubMed/NCBI

14 

McGlacken GP and Fairlamb IJ: 2-Pyrone natural products and mimetics: Isolation, characterisation and biological activity. Nat Prod Rep. 22:369–385. 2005. View Article : Google Scholar : PubMed/NCBI

15 

Dickinson JM: Microbial pyran-2-ones and dihydropyran-2-ones. Nat Prod Rep. 10:71–98. 1993. View Article : Google Scholar : PubMed/NCBI

16 

Thaisrivongs S, Romero DL, Tommasi RA, Janakiraman MN, Strohbach JW, Turner SR, Biles C, Morge RR, Johnson PD, Aristoff PA, et al: Structure-based design of HIV protease inhibitors: 5,6-dihydro-4-hydroxy-2-pyrones as effective, nonpeptidic inhibitors. J Med Chem. 39:4630–4642. 1996. View Article : Google Scholar : PubMed/NCBI

17 

Poppe SM, Slade DE, Chong KT, Hinshaw RR, Pagano PJ, Markowitz M, Ho DD, Mo H, Gorman RR III, Dueweke TJ, et al: Antiviral activity of the dihydropyrone PNU-140690, a new nonpeptidic human immunodeficiency virus protease inhibitor. Antimicrob Agents Chemother. 41:1058–1063. 1997.PubMed/NCBI

18 

Turner SR, Strohbach JW, Tommasi RA, Aristoff PA, Johnson PD, Skulnick HI, Dolak LA, Seest EP, Tomich PK, Bohanon MJ, et al: Tipranavir (PNU-140690): A potent, orally bioavailable nonpeptidic HIV protease inhibitor of the 5,6-dihydro-4-hydroxy-2-pyrone sulfonamide class. J Med Chem. 41:3467–3476. 1998. View Article : Google Scholar : PubMed/NCBI

19 

Trachootham D, Lu W, Ogasawara MA, Nilsa RD and Huang P: Redox regulation of cell survival. Antioxid Redox Signal. 10:1343–1374. 2008. View Article : Google Scholar : PubMed/NCBI

20 

Hartwell LH and Weinert TA: Checkpoints: Controls that ensure the order of cell cycle events. Science. 246:629–634. 1989. View Article : Google Scholar : PubMed/NCBI

21 

Adams JM and Cory S: The Bcl-2 protein family: Arbiters of cell survival. Science. 281:1322–1326. 1998. View Article : Google Scholar : PubMed/NCBI

22 

Antonsson B and Martinou JC: The Bcl-2 protein family. Exp Cell Res. 256:50–57. 2000. View Article : Google Scholar : PubMed/NCBI

23 

Salvesen GS and Dixit VM: Caspases: Intracellular signaling by proteolysis. Cell. 91:443–446. 1997. View Article : Google Scholar : PubMed/NCBI

24 

Rauh R, Kahl S, Boechzelt H, Bauer R, Kaina B and Efferth T: Molecular biology of cantharidin in cancer cells. Chin Med. 2:82007. View Article : Google Scholar : PubMed/NCBI

25 

Ozben T: Oxidative stress and apoptosis: Impact on cancer therapy. J Pharm Sci. 96:2181–2196. 2007. View Article : Google Scholar : PubMed/NCBI

26 

Xiao L, Eto M and Kazanietz MG: ROCK mediates phorbol ester-induced apoptosis in prostate cancer cells via p21Cip1 upregulation and JNK. J Biol Chem. 284:29365–29375. 2009. View Article : Google Scholar : PubMed/NCBI

27 

Lin KL, Su JC, Chien CM, Tseng CH, Chen YL, Chang LS and Lin SR: Naphtho [1,2-b]furan-4,5-dione induces apoptosis and S-phase arrest of MDA-MB-231 cells through JNK and ERK signaling activation. Toxicol In Vitro. 24:61–70. 2010. View Article : Google Scholar : PubMed/NCBI

28 

Choi YJ, Kim SY, Oh JM and Juhnn YS: Stimulatory heterotrimeric G protein augments gamma ray-induced apoptosis by up-regulation of Bak expression via CREB and AP-1 in HL299 human lung cancer cells. Exp Mol Med. 41:592–600. 2009. View Article : Google Scholar : PubMed/NCBI

29 

An L, Dong GQ, Gao Q, Zhang Y, Hu LW, Li JH and Liu Y: Effects of UVA on TNF-alpha, IL-1beta, and IL-10 expression levels in human keratinocytes and intervention studies with an antioxidant and a JNK inhibitor. Photodermatol Photoimmunol Photomed. 26:28–35. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yu GQ, Dou ZL and Jia ZH: 5‑bromo‑3‑(3‑hydroxyprop‑1‑ynyl)‑2H‑pyran‑2‑one induces apoptosis in T24 human bladder cancer cells through mitochondria-dependent signaling pathways Corrigendum in /10.3892/mmr.2016.6076. Mol Med Rep 15: 153-159, 2017.
APA
Yu, G., Dou, Z., & Jia, Z. (2017). 5‑bromo‑3‑(3‑hydroxyprop‑1‑ynyl)‑2H‑pyran‑2‑one induces apoptosis in T24 human bladder cancer cells through mitochondria-dependent signaling pathways Corrigendum in /10.3892/mmr.2016.6076. Molecular Medicine Reports, 15, 153-159. https://doi.org/10.3892/mmr.2016.5991
MLA
Yu, G., Dou, Z., Jia, Z."5‑bromo‑3‑(3‑hydroxyprop‑1‑ynyl)‑2H‑pyran‑2‑one induces apoptosis in T24 human bladder cancer cells through mitochondria-dependent signaling pathways Corrigendum in /10.3892/mmr.2016.6076". Molecular Medicine Reports 15.1 (2017): 153-159.
Chicago
Yu, G., Dou, Z., Jia, Z."5‑bromo‑3‑(3‑hydroxyprop‑1‑ynyl)‑2H‑pyran‑2‑one induces apoptosis in T24 human bladder cancer cells through mitochondria-dependent signaling pathways Corrigendum in /10.3892/mmr.2016.6076". Molecular Medicine Reports 15, no. 1 (2017): 153-159. https://doi.org/10.3892/mmr.2016.5991
Copy and paste a formatted citation
x
Spandidos Publications style
Yu GQ, Dou ZL and Jia ZH: 5‑bromo‑3‑(3‑hydroxyprop‑1‑ynyl)‑2H‑pyran‑2‑one induces apoptosis in T24 human bladder cancer cells through mitochondria-dependent signaling pathways Corrigendum in /10.3892/mmr.2016.6076. Mol Med Rep 15: 153-159, 2017.
APA
Yu, G., Dou, Z., & Jia, Z. (2017). 5‑bromo‑3‑(3‑hydroxyprop‑1‑ynyl)‑2H‑pyran‑2‑one induces apoptosis in T24 human bladder cancer cells through mitochondria-dependent signaling pathways Corrigendum in /10.3892/mmr.2016.6076. Molecular Medicine Reports, 15, 153-159. https://doi.org/10.3892/mmr.2016.5991
MLA
Yu, G., Dou, Z., Jia, Z."5‑bromo‑3‑(3‑hydroxyprop‑1‑ynyl)‑2H‑pyran‑2‑one induces apoptosis in T24 human bladder cancer cells through mitochondria-dependent signaling pathways Corrigendum in /10.3892/mmr.2016.6076". Molecular Medicine Reports 15.1 (2017): 153-159.
Chicago
Yu, G., Dou, Z., Jia, Z."5‑bromo‑3‑(3‑hydroxyprop‑1‑ynyl)‑2H‑pyran‑2‑one induces apoptosis in T24 human bladder cancer cells through mitochondria-dependent signaling pathways Corrigendum in /10.3892/mmr.2016.6076". Molecular Medicine Reports 15, no. 1 (2017): 153-159. https://doi.org/10.3892/mmr.2016.5991
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