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Differentially expressed miRNAs in oxygen‑induced retinopathy newborn mouse models

  • Authors:
    • Yunpeng Wang
    • Suying Wu
    • Yang Yang
    • Fen Peng
    • Qintao Li
    • Peng Tian
    • Erying Xiang
    • Honglu Liang
    • Beibei Wang
    • Xiaoyu Zhou
    • Hua Huang
    • Xiaoguang Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Neonatology, Nanshan People's Hospital, Affiliated to Guangdong Medical University, Shenzhen, Guangdong 518052, P.R. China, Department of Neonatology, University Hospital of Hubei Minzu University, Enshi, Hubei 445000, P.R. China, Department of Neonatology, Children's Hospital of Nanjing Medical University, Nanjing, Jiangsu 210008, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 146-152
    |
    Published online on: December 6, 2016
       https://doi.org/10.3892/mmr.2016.5993
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Abstract

The present study aimed to identify microRNAs (miRNAs) involved in regulating retinal neovascularization and retinopathy of prematurity (ROP). A total of 80 healthy C57BL/6 neonatal mice were randomly divided into the oxygen‑induced retinopathy (OIR) group (n=40), in which 7‑day‑old mice were maintained in 75% oxygen conditions for 5 days, or the control group (n=40). Following collection of retinal tissue, retinal angiography and hematoxylin and eosin (H&E) staining were performed. Total RNA was also extracted from retinal tissue, and miRNA microarrays and reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) were performed to identify differentially expressed miRNAs in the two groups. Retinal angiography and H&E staining revealed damage to retinas in the OIR group. Compared with the control group, 67 miRNAs were differentially expressed in the OIR group, of which 34 were upregulated and 33 were downregulated. Of these differentially expressed miRNAs, 32 exhibited a fold change ≥2, of which 21 were upregulated and 11 were downregulated. The results of RT‑qPCR for miR‑130a‑3p and miR‑5107‑5p were in accordance with those of the miRNA microarray. The newly identified miRNAs may be important in the development of ROP, and may provide a basis for future research into the mechanisms of ROP.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Y, Wu S, Yang Y, Peng F, Li Q, Tian P, Xiang E, Liang H, Wang B, Zhou X, Zhou X, et al: Differentially expressed miRNAs in oxygen‑induced retinopathy newborn mouse models. Mol Med Rep 15: 146-152, 2017.
APA
Wang, Y., Wu, S., Yang, Y., Peng, F., Li, Q., Tian, P. ... Zhou, X. (2017). Differentially expressed miRNAs in oxygen‑induced retinopathy newborn mouse models. Molecular Medicine Reports, 15, 146-152. https://doi.org/10.3892/mmr.2016.5993
MLA
Wang, Y., Wu, S., Yang, Y., Peng, F., Li, Q., Tian, P., Xiang, E., Liang, H., Wang, B., Zhou, X., Huang, H., Zhou, X."Differentially expressed miRNAs in oxygen‑induced retinopathy newborn mouse models". Molecular Medicine Reports 15.1 (2017): 146-152.
Chicago
Wang, Y., Wu, S., Yang, Y., Peng, F., Li, Q., Tian, P., Xiang, E., Liang, H., Wang, B., Zhou, X., Huang, H., Zhou, X."Differentially expressed miRNAs in oxygen‑induced retinopathy newborn mouse models". Molecular Medicine Reports 15, no. 1 (2017): 146-152. https://doi.org/10.3892/mmr.2016.5993
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Wu S, Yang Y, Peng F, Li Q, Tian P, Xiang E, Liang H, Wang B, Zhou X, Zhou X, et al: Differentially expressed miRNAs in oxygen‑induced retinopathy newborn mouse models. Mol Med Rep 15: 146-152, 2017.
APA
Wang, Y., Wu, S., Yang, Y., Peng, F., Li, Q., Tian, P. ... Zhou, X. (2017). Differentially expressed miRNAs in oxygen‑induced retinopathy newborn mouse models. Molecular Medicine Reports, 15, 146-152. https://doi.org/10.3892/mmr.2016.5993
MLA
Wang, Y., Wu, S., Yang, Y., Peng, F., Li, Q., Tian, P., Xiang, E., Liang, H., Wang, B., Zhou, X., Huang, H., Zhou, X."Differentially expressed miRNAs in oxygen‑induced retinopathy newborn mouse models". Molecular Medicine Reports 15.1 (2017): 146-152.
Chicago
Wang, Y., Wu, S., Yang, Y., Peng, F., Li, Q., Tian, P., Xiang, E., Liang, H., Wang, B., Zhou, X., Huang, H., Zhou, X."Differentially expressed miRNAs in oxygen‑induced retinopathy newborn mouse models". Molecular Medicine Reports 15, no. 1 (2017): 146-152. https://doi.org/10.3892/mmr.2016.5993
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