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Age-associated expression of erythropoietin and its receptor in rat spiral ganglion neurons and its association with neuronal apoptosis and hearing alterations

  • Authors:
    • Cheng Zhong
    • Xueyuan Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Otolaryngology, Southwest Hospital, Third Military Medical University, Chongqing 400038, P.R. China
    Copyright: © Zhong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 228-234
    |
    Published online on: December 9, 2016
       https://doi.org/10.3892/mmr.2016.6010
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Abstract

The present study aimed to determine the expression of erythropoietin (EPO) and the EPO receptor (EPOR) in spiral ganglion neurons (SGNs) in the inner ear of rats of various ages, and the associated neuronal apoptosis and hearing alterations. A total of 15 healthy rats (n=30 ears), were divided into three groups: i) A nominated infant group at post‑natal day (PND) 12‑14, ii) an adult group at PND 60 and iii) a 3‑year postnatal aged group. Auditory brainstem response (ABR) measurements were performed on all rats. EPO and EPOR expression in the inner ear was detected by immunohistochemistry. In situ terminal deoxynucleotidyl transferase dUTP nick end labeling assays were performed to detect the apoptosis of SGNs. The average hearing thresholds of the ABR (decibels above normal hearing level) were 5.625±4.955 in the infant, 15.000±8.498 in the adult and 23.500±13.134 in the aged groups. Hearing thresholds for aged and adult rats increased significantly compared with infant rats. However, the difference in latencies of peak I was not significant (P>0.05). EPO in SGNs was detected during different developmental periods without significant alterations, but were reduced compared with Corti's organ or the stria vascularis. EPOR expression increased significantly from infant to adult stage, and this increased expression was maintained in the aged group. An age‑associated increase in the apoptosis of SGNs was detected in all three groups (P=0.0347). The potential neuroprotective effects of EPO in SGNs may not be revealed during the aging process under natural conditions, and may be associated with spontaneous neuronal apoptosis and consequently, hearing diminution. However, the age‑associated increase in EPOR in SGNs may exert a role in neuroprotection when necessary, for example in presbycusis.
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1 

Monini S, Filippi C, Baldini R and Barbara M: Perceived disability from hearing and voice changes in the elderly. Geriatr Gerontol Int. 15:147–155. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Yamasoba T, Lin FR, Someya S, Kashio A, Sakamoto T and Kondo K: Current concepts in age-related hearing loss: Epidemiology and mechanistic pathways. Hear Res. 303:30–38. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Bao J and Ohlemiller KK: Age-related loss of spiral ganglion neurons. Hear Res. 264:93–97. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Byts N and Sirén AL: Erythropoietin: A multimodal neuroprotective agent. Exp Transl Stroke Med. 1:42009. View Article : Google Scholar : PubMed/NCBI

5 

Brines M and Cerami A: Discovering erythropoietin's extra-hematopoietic functions: Biology and clinical promise. Kidney Int. 70:246–250. 2006. View Article : Google Scholar : PubMed/NCBI

6 

Cayé-Thomasen P, Wagner N, Frederiksen B Lidegaard, Asal K and Thomsen J: Erythropoietin and erythropoietin receptor expression in the guinea pig inner ear. Hear Res. 203:21–27. 2005. View Article : Google Scholar : PubMed/NCBI

7 

Berkingali N, Warnecke A, Gomes P, Paasche G, Tack J, Lenarz T and Stöver T: Neurite outgrowth on cultured spiral ganglion neurons induced by erythropoietin. Hear Res. 243:121–126. 2008. View Article : Google Scholar : PubMed/NCBI

8 

Naldi A Monge, Gassmann M and Bodmer D: Erythropoietin but not VEGF has a protective effect on auditory hair cells in the inner ear. Cell Mol Life Sci. 66:3595–3599. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Panza F, Solfrizzi V and Logroscino G: Age-related hearing impairment-a risk factor and frailty marker for dementia and AD. Nat Rev Neurol. 11:166–175. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Jewett DL and Williston JS: Auditory-evoked far fields averaged from the scalp of humans. Brain. 94:681–696. 1971. View Article : Google Scholar : PubMed/NCBI

11 

Malherbe TK, Hanekom T and Hanekom JJ: Can subject-specific single-fibreelectrically evoked auditory brainstem response data be predicted from a model? Med Eng Phys. 35:926–936. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Rattay F and Danner SM: Peak I of the human auditory brainstem response results from the somatic regions of type I spiral ganglion cells: Evidence from computer modeling. Hear Res. 315:67–79. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Brown DJ and Patuzzi RB: Evidence that the compound action potential (CAP) from the auditory nerve is a stationary potential generated across dura mater. Hear Res. 267:12–26. 2010. View Article : Google Scholar : PubMed/NCBI

14 

Chong ZZ and Maiese K: Erythropoietin involves the phosphatidylinositol 3-kinase pathway, 14-4-4 protein and FOXO3a nuclear traffic-king to preserve endothelia cell integrity. Br J Pharmacol. 150:839–850. 2007. View Article : Google Scholar : PubMed/NCBI

15 

Sims B, Clarke M, Njah W, Hopkins ES and Sontheimer H: Erythropoietin-induced neuroprotection requires cystine glutamate exchanger activity. Brain Res. 1321:88–95. 2010. View Article : Google Scholar : PubMed/NCBI

16 

Gross J, Moller R, Amarjargal N, Machulik A, Fuchs J, Ungethüm U, Kuban RJ, Henke W, Haupt H and Mazurek B: Expression of erythropoietin and angiogenic growth factors following inner ear injury of newborn rats. Prague Med Rep. 110:310–331. 2009.PubMed/NCBI

17 

Kaiser O, Paasche G, Stöver T, Ernst S, Lenarz T, Kral A and Warnecke A: TGF-beta superfamily member activin A acts with BDNF and erythropoietin to improve survival of spiral ganglion neurons in vitro. Neuropharma. 75:416–425. 2013. View Article : Google Scholar

18 

Juul SE, Stallings SA and Christensen RD: Erythropoieti n in the cerebrospinal fluid of neonates who sustained CNS injury. Pediatr Res. 46:543–547. 1999. View Article : Google Scholar : PubMed/NCBI

19 

Saunders NR, Habgood MD and Dziegielewska KM: Barrier mechanisms in the brain, II. Immature brain. Clin Exp Pharmacol Physiol. 26:85–91. 1999. View Article : Google Scholar : PubMed/NCBI

20 

Banks WA, Jumbe NL, Farrell CL, Niehoff ML and Heatherington AC: Passage of erythropoietic agents across the blood-brain barrier: A comparison of human and murine erythropoietin and the analog darbepoetin alfa. Eur J Pharmacol. 505:93–101. 2004. View Article : Google Scholar : PubMed/NCBI

21 

Frederiksen BL, Cayé-Thomasen P, Lund SP, Wagner N, Asal K, Olsen NV and Thomsen J: Does erythropoietin augment noise induced hearing loss? Hear Res. 223:129–137. 2007. View Article : Google Scholar : PubMed/NCBI

22 

Maiese K, Chong ZZ, Li F and Shang YC: Erythropoietin: Elucidating new cellular targets that broaden therapeutic strategies. Prog Neurobiol. 85:194–213. 2008. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhong C and Zhang X: Age-associated expression of erythropoietin and its receptor in rat spiral ganglion neurons and its association with neuronal apoptosis and hearing alterations. Mol Med Rep 15: 228-234, 2017.
APA
Zhong, C., & Zhang, X. (2017). Age-associated expression of erythropoietin and its receptor in rat spiral ganglion neurons and its association with neuronal apoptosis and hearing alterations. Molecular Medicine Reports, 15, 228-234. https://doi.org/10.3892/mmr.2016.6010
MLA
Zhong, C., Zhang, X."Age-associated expression of erythropoietin and its receptor in rat spiral ganglion neurons and its association with neuronal apoptosis and hearing alterations". Molecular Medicine Reports 15.1 (2017): 228-234.
Chicago
Zhong, C., Zhang, X."Age-associated expression of erythropoietin and its receptor in rat spiral ganglion neurons and its association with neuronal apoptosis and hearing alterations". Molecular Medicine Reports 15, no. 1 (2017): 228-234. https://doi.org/10.3892/mmr.2016.6010
Copy and paste a formatted citation
x
Spandidos Publications style
Zhong C and Zhang X: Age-associated expression of erythropoietin and its receptor in rat spiral ganglion neurons and its association with neuronal apoptosis and hearing alterations. Mol Med Rep 15: 228-234, 2017.
APA
Zhong, C., & Zhang, X. (2017). Age-associated expression of erythropoietin and its receptor in rat spiral ganglion neurons and its association with neuronal apoptosis and hearing alterations. Molecular Medicine Reports, 15, 228-234. https://doi.org/10.3892/mmr.2016.6010
MLA
Zhong, C., Zhang, X."Age-associated expression of erythropoietin and its receptor in rat spiral ganglion neurons and its association with neuronal apoptosis and hearing alterations". Molecular Medicine Reports 15.1 (2017): 228-234.
Chicago
Zhong, C., Zhang, X."Age-associated expression of erythropoietin and its receptor in rat spiral ganglion neurons and its association with neuronal apoptosis and hearing alterations". Molecular Medicine Reports 15, no. 1 (2017): 228-234. https://doi.org/10.3892/mmr.2016.6010
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