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Article

Engineering of the human vessel wall with hair follicle stem cells in vitro

  • Authors:
    • Zhi‑Cheng Xu
    • Qun Zhang
    • Hong Li
  • View Affiliations / Copyright

    Affiliations: Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, P.R. China, Department of Life Information and Instrument Engineering, Hangzhou Electronic Science and Technology University, Hangzhou, Zhejiang 310058, P.R. China
  • Pages: 417-422
    |
    Published online on: December 9, 2016
       https://doi.org/10.3892/mmr.2016.6013
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Abstract

Hair follicle stem cells (HFSCs) are increasingly used as a stem cell paradigm in vascular tissue engineering due to the fact that they are a rich source of easily accessible multipotent adult stem cells. Promising results have been demonstrated with small diameter (less than 6 mm) tissue engineered blood vessels under low blood pressure, however engineering large vessels (>6 mm in diameter) remains a challenge due to the fact it demands a higher number of seed cells and higher quality biomechanical properties. The aim of the current study was to engineer a large vessel (6 mm in diameter) with differentiated smooth muscle cells (SMCs) induced from human (h)HFSCs using transforming growth factor‑β1 and platelet‑derived growth factor BB in combination with low‑serum culture medium. The cells were seeded onto polyglycolic acid and then wrapped around a silicone tube and further cultured in vitro. A round vessel wall was formed subsequent to 8 weeks of culture. Histological examination indicated that layers of smooth muscle‑like cells and collagenous fibres were oriented in the induced group. In contrast, disorganised cells and collagenous fibres were apparent in the undifferentiated group. The approach developed in the current study demonstrated potential for constructing large muscular vessels with differentiated SMCs induced from hHFSCs.
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Copy and paste a formatted citation
Spandidos Publications style
Xu ZC, Zhang Q and Li H: Engineering of the human vessel wall with hair follicle stem cells in vitro. Mol Med Rep 15: 417-422, 2017.
APA
Xu, Z., Zhang, Q., & Li, H. (2017). Engineering of the human vessel wall with hair follicle stem cells in vitro. Molecular Medicine Reports, 15, 417-422. https://doi.org/10.3892/mmr.2016.6013
MLA
Xu, Z., Zhang, Q., Li, H."Engineering of the human vessel wall with hair follicle stem cells in vitro". Molecular Medicine Reports 15.1 (2017): 417-422.
Chicago
Xu, Z., Zhang, Q., Li, H."Engineering of the human vessel wall with hair follicle stem cells in vitro". Molecular Medicine Reports 15, no. 1 (2017): 417-422. https://doi.org/10.3892/mmr.2016.6013
Copy and paste a formatted citation
x
Spandidos Publications style
Xu ZC, Zhang Q and Li H: Engineering of the human vessel wall with hair follicle stem cells in vitro. Mol Med Rep 15: 417-422, 2017.
APA
Xu, Z., Zhang, Q., & Li, H. (2017). Engineering of the human vessel wall with hair follicle stem cells in vitro. Molecular Medicine Reports, 15, 417-422. https://doi.org/10.3892/mmr.2016.6013
MLA
Xu, Z., Zhang, Q., Li, H."Engineering of the human vessel wall with hair follicle stem cells in vitro". Molecular Medicine Reports 15.1 (2017): 417-422.
Chicago
Xu, Z., Zhang, Q., Li, H."Engineering of the human vessel wall with hair follicle stem cells in vitro". Molecular Medicine Reports 15, no. 1 (2017): 417-422. https://doi.org/10.3892/mmr.2016.6013
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