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Effects of herbal Epimedium on the improvement of bone metabolic disorder through the induction of osteogenic differentiation from bone marrow-derived mesenchymal stem cells

  • Authors:
    • Do Rim Kim
    • Ji Eun Lee
    • Kyung Jun Shim
    • Jin Hyoung Cho
    • Ho Chul Lee
    • Seong Kyu Park
    • Mun Seog Chang
  • View Affiliations / Copyright

    Affiliations: Department of Prescriptionology, College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea
    Copyright: © Kim et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 125-130
    |
    Published online on: December 9, 2016
       https://doi.org/10.3892/mmr.2016.6015
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Abstract

Herbal Epimedium (HE) has been commonly used as a tonic, antirheumatic agent and in the treatment of bone‑associated diseases including osteoporosis. Treatment for osteoporosis is important to increase bone mass density and maintain to balance of bone remodeling. The present study was performed to investigate the effects of HE on mouse bone marrow mesenchymal stem cell (mBMMSC) proliferation and osteogenic differentiation, using MTT assays, proliferating cell nuclear antigen (PCNA) detection and apoptosis and differentiation assays. HE was demonstrated to inhibit the proliferation of mBMMSCs up to 45.43±3.33% and to decrease the level of PCNA expression compared with untreated cells. HE also induced late apoptosis at 24 and 48 h after treatment up to 71.93 and 67.03%, respectively, while only 14.93% of untreated cells exhibited apoptosis. By contrast, HE induced differentiation of mBMMSCs into an osteogenic lineage at the beginning of three weeks after commencement of treatment. This suggested that HE is a candidate as an inducer of osteogenesis from bone marrow mesenchymal stem cells, and additionally has potential for use in the treatment of bone metabolic disorders such as osteoporosis.
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1 

Gennari L, Rotatori S, Bianciardi S, Gonnelli S, Nuti R and Merlotti D: Appropriate models for novel osteoporosis drug discovery and future perspectives. Expert Opin Drug Discov. 10:1201–1216. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Sambrook P and Cooper C: Osteoporosis. Lancet. 17:2010–2018. 2006.

3 

Arceo-Mendoza RM and Camacho P: Prediction of fracture risk in patients with osteoporosis: A brief review. Womens Health (Lond Engl). 11:477–482. 2015. View Article : Google Scholar

4 

Iñiguez-Ariza NM and Clarke BL: Bone biology, signaling pathways, and therapeutic targets for osteoporosis. Maturitas. 82:245–255. 2015. View Article : Google Scholar : PubMed/NCBI

5 

Mikami Y, Matsumoto T, Kano K, Toriumi T, Somei M, Honda MJ and Komiyama K: Current status of drug therapies for osteoporosis and the search for stem cells adapted for bone regenerative medicine. Anat Sci Int. 89:1–10. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Vitale AM, Wolvetang E and Mackay-Sim A: Induced pluripotent stem cells: A new technology to study human diseases. Int J Biochem Cell Biol. 43:843–846. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Tögel F and Westenfelder C: Adult bone marrow-derived stem cells for organ regeneration and repair. Dev Dyn. 236:3321–3331. 2007. View Article : Google Scholar : PubMed/NCBI

8 

Hentze H, Graichen R and Colman A: Cell therapy and the safety of embryonic stem cell-derived grafts. Trends Biotechnol. 25:24–32. 2007. View Article : Google Scholar : PubMed/NCBI

9 

Chamberlain G, Fox J, Ashton B and Middleton J: Concise review: Mesenchymal stem cells: Their phenotype, differentiation capacity, immunological features, and potential for homing. Stem Cells. 25:2739–2749. 2007. View Article : Google Scholar : PubMed/NCBI

10 

Ozawa K, Sato K, Oh I, Ozaki K, Uchibori R, Obara Y, Kikuchi Y, Ito T, Okada T, Urabe M, et al: Cell and gene therapy using mesenchymal stem cells (MSCs). J Autoimmun. 30:121–127. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Jethva R, Otsuru S, Dominici M and Horwitz EM: Cell therapy for disorders of bone. Cytotherapy. 11:3–17. 2009. View Article : Google Scholar : PubMed/NCBI

12 

Van Damme A, Driessche T Vanden, Collen D and Chuah MK: Bone marrow stromal cells as targets for gene therapy. Curr Gene Ther. 2:195–209. 2002. View Article : Google Scholar : PubMed/NCBI

13 

Yan FF, Liu Y, Liu YF and Zhao YX: Herba epimedii water extract elevates estrogen level and improves lipid metabolism in postmenopausal women. Phytother Res. 22:1224–1228. 2008. View Article : Google Scholar : PubMed/NCBI

14 

Sze SC, Tong Y, Ng TB, Cheng CL and Cheung HP: Herba epimedii: Anti-oxidative properties and its medical implications. Molecules. 15:7861–7870. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Chen WF, Mok SK, Wang XL, Lai KH, Lai WP, Luk HK, Leung PC, Yao XS and Wong MS: Total flavonoid action of the Herba epimedii extract suppresses urinary calcium excretion and improves bone properties in ovariectomised mice. Br J Nutr. 105:180–189. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Nadri S and Soleimani M: Isolation murine mesenchymal stem cells by positive selection. In Vitro Cell Dev Biol Anim. 43:276–282. 2007. View Article : Google Scholar : PubMed/NCBI

17 

Eslaminejad MB and Nadri S: Murine mesenchymal stem cell isolated and expanded in low and high density culture system: Surface antigen expression and osteogenic culture mineralization. In Vitro Cell Dev Biol Anim. 45:451–459. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Bonab MM, Alimoghaddam K, Talebian F, Ghaffari SH, Ghavamzadeh A and Nikbin B: Aging of mesenchymal stem cells in vitro. BMC Cell Biol. 7:142006. View Article : Google Scholar : PubMed/NCBI

19 

Yee AS, Paulson EK, McDevitt MA, Rieger-Christ K, Summerhayes I, Berasi SP, Kim J, Huang CY and Zhang X: The HBP1 transcriptional repressor and the p38 MAP kinase: Unlikely partners in G1 regulation and tumor suppression. Gene. 336:1–13. 2004. View Article : Google Scholar : PubMed/NCBI

20 

Oliver L, Hue E, Rossignol J, Bougras G, Hulin P, Naveilhan P, Heymann D, Lescaudron L and Vallette FM: Distinct roles of Bcl-2 and Bcl-Xl in the apoptosis of human bone marrow mesenchymal stem cells during differentiation. PLoS One. 6:e198202011. View Article : Google Scholar : PubMed/NCBI

21 

Napolitano MA, Cipollaro M, Cascino A, Melone MA, Giordano A and Galderisi U: Brg1 chromatin remodeling factor is involved in cell growth arrest, apoptosis and senescence of rat mesenchymal stem cells. J Cell Sci. 15:2904–2911. 2007.

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Copy and paste a formatted citation
Spandidos Publications style
Kim DR, Lee JE, Shim KJ, Cho JH, Lee HC, Park SK and Chang MS: Effects of herbal Epimedium on the improvement of bone metabolic disorder through the induction of osteogenic differentiation from bone marrow-derived mesenchymal stem cells. Mol Med Rep 15: 125-130, 2017.
APA
Kim, D.R., Lee, J.E., Shim, K.J., Cho, J.H., Lee, H.C., Park, S.K., & Chang, M.S. (2017). Effects of herbal Epimedium on the improvement of bone metabolic disorder through the induction of osteogenic differentiation from bone marrow-derived mesenchymal stem cells. Molecular Medicine Reports, 15, 125-130. https://doi.org/10.3892/mmr.2016.6015
MLA
Kim, D. R., Lee, J. E., Shim, K. J., Cho, J. H., Lee, H. C., Park, S. K., Chang, M. S."Effects of herbal Epimedium on the improvement of bone metabolic disorder through the induction of osteogenic differentiation from bone marrow-derived mesenchymal stem cells". Molecular Medicine Reports 15.1 (2017): 125-130.
Chicago
Kim, D. R., Lee, J. E., Shim, K. J., Cho, J. H., Lee, H. C., Park, S. K., Chang, M. S."Effects of herbal Epimedium on the improvement of bone metabolic disorder through the induction of osteogenic differentiation from bone marrow-derived mesenchymal stem cells". Molecular Medicine Reports 15, no. 1 (2017): 125-130. https://doi.org/10.3892/mmr.2016.6015
Copy and paste a formatted citation
x
Spandidos Publications style
Kim DR, Lee JE, Shim KJ, Cho JH, Lee HC, Park SK and Chang MS: Effects of herbal Epimedium on the improvement of bone metabolic disorder through the induction of osteogenic differentiation from bone marrow-derived mesenchymal stem cells. Mol Med Rep 15: 125-130, 2017.
APA
Kim, D.R., Lee, J.E., Shim, K.J., Cho, J.H., Lee, H.C., Park, S.K., & Chang, M.S. (2017). Effects of herbal Epimedium on the improvement of bone metabolic disorder through the induction of osteogenic differentiation from bone marrow-derived mesenchymal stem cells. Molecular Medicine Reports, 15, 125-130. https://doi.org/10.3892/mmr.2016.6015
MLA
Kim, D. R., Lee, J. E., Shim, K. J., Cho, J. H., Lee, H. C., Park, S. K., Chang, M. S."Effects of herbal Epimedium on the improvement of bone metabolic disorder through the induction of osteogenic differentiation from bone marrow-derived mesenchymal stem cells". Molecular Medicine Reports 15.1 (2017): 125-130.
Chicago
Kim, D. R., Lee, J. E., Shim, K. J., Cho, J. H., Lee, H. C., Park, S. K., Chang, M. S."Effects of herbal Epimedium on the improvement of bone metabolic disorder through the induction of osteogenic differentiation from bone marrow-derived mesenchymal stem cells". Molecular Medicine Reports 15, no. 1 (2017): 125-130. https://doi.org/10.3892/mmr.2016.6015
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