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Article Open Access

Ganoderma lucidum polysaccharides protect fibroblasts against UVB-induced photoaging

  • Authors:
    • Qinghai Zeng
    • Fang Zhou
    • Li Lei
    • Jing Chen
    • Jianyun Lu
    • Jianda Zhou
    • Ke Cao
    • Lihua Gao
    • Fang Xia
    • Shu Ding
    • Lihua Huang
    • Hong Xiang
    • Jingjing Wang
    • Yangfan Xiao
    • Rong Xiao
    • Jinhua Huang
  • View Affiliations / Copyright

    Affiliations: Department of Dermatology, Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China, Department of Burn and Plastic Surgery, Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China, Department of Oncology, Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China, The Central Laboratory, Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China, Department of Dermatology, Second Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China
    Copyright: © Zeng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 111-116
    |
    Published online on: December 12, 2016
       https://doi.org/10.3892/mmr.2016.6026
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Abstract

Ganoderma lucidum has featured in traditional Chinese medicine for >1,000 years. Ganoderma polysaccharides (GL-PS), a major active ingredient in Ganoderma, confer immune regulation, antitumor effects and significant antioxidant effects. The aim of the present study was to investigate the efficacy and mechanism of GL‑PS‑associated inhibition of ultraviolet B (UVB)‑induced photoaging in human fibroblasts in vitro. Primary human skin fibroblasts were cultured, and a fibroblast photoaging model was built through exposure to UVB. Cell viability was measured by MTT assay. Aged cells were stained using a senescence‑associated β-galactosidase staining (SA‑β‑gal) kit. ELISA kits were used to analyze matrix metalloproteinase (MMP) ‑1 and C‑telopeptides of Type I collagen (CICP) protein levels in cellular supernatant. ROS levels were quantified by flow cytometry. Cells exposed to UVB had decreased cell viability, increased aged cells, decreased CICP protein expression, increased MMP‑1 protein expression, and increased cellular ROS levels compared with non‑exposed cells. However, cells exposed to UVB and treated with 10, 20 and 40 µg/ml GL‑PS demonstrated increased cell viability, decreased aged cells, increased CICP protein expression, decreased MMP‑1 protein expression, and decreased cellular ROS levels compared with UVB exposed/GL‑PS untreated cells. These results demonstrate that GL‑PS protects fibroblasts against photoaging by eliminating UVB‑induced ROS. This finding indicates GL‑PS treatment may serve as a novel strategy for antiphotoaging.
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Copy and paste a formatted citation
Spandidos Publications style
Zeng Q, Zhou F, Lei L, Chen J, Lu J, Zhou J, Cao K, Gao L, Xia F, Ding S, Ding S, et al: Ganoderma lucidum polysaccharides protect fibroblasts against UVB-induced photoaging. Mol Med Rep 15: 111-116, 2017.
APA
Zeng, Q., Zhou, F., Lei, L., Chen, J., Lu, J., Zhou, J. ... Huang, J. (2017). Ganoderma lucidum polysaccharides protect fibroblasts against UVB-induced photoaging. Molecular Medicine Reports, 15, 111-116. https://doi.org/10.3892/mmr.2016.6026
MLA
Zeng, Q., Zhou, F., Lei, L., Chen, J., Lu, J., Zhou, J., Cao, K., Gao, L., Xia, F., Ding, S., Huang, L., Xiang, H., Wang, J., Xiao, Y., Xiao, R., Huang, J."Ganoderma lucidum polysaccharides protect fibroblasts against UVB-induced photoaging". Molecular Medicine Reports 15.1 (2017): 111-116.
Chicago
Zeng, Q., Zhou, F., Lei, L., Chen, J., Lu, J., Zhou, J., Cao, K., Gao, L., Xia, F., Ding, S., Huang, L., Xiang, H., Wang, J., Xiao, Y., Xiao, R., Huang, J."Ganoderma lucidum polysaccharides protect fibroblasts against UVB-induced photoaging". Molecular Medicine Reports 15, no. 1 (2017): 111-116. https://doi.org/10.3892/mmr.2016.6026
Copy and paste a formatted citation
x
Spandidos Publications style
Zeng Q, Zhou F, Lei L, Chen J, Lu J, Zhou J, Cao K, Gao L, Xia F, Ding S, Ding S, et al: Ganoderma lucidum polysaccharides protect fibroblasts against UVB-induced photoaging. Mol Med Rep 15: 111-116, 2017.
APA
Zeng, Q., Zhou, F., Lei, L., Chen, J., Lu, J., Zhou, J. ... Huang, J. (2017). Ganoderma lucidum polysaccharides protect fibroblasts against UVB-induced photoaging. Molecular Medicine Reports, 15, 111-116. https://doi.org/10.3892/mmr.2016.6026
MLA
Zeng, Q., Zhou, F., Lei, L., Chen, J., Lu, J., Zhou, J., Cao, K., Gao, L., Xia, F., Ding, S., Huang, L., Xiang, H., Wang, J., Xiao, Y., Xiao, R., Huang, J."Ganoderma lucidum polysaccharides protect fibroblasts against UVB-induced photoaging". Molecular Medicine Reports 15.1 (2017): 111-116.
Chicago
Zeng, Q., Zhou, F., Lei, L., Chen, J., Lu, J., Zhou, J., Cao, K., Gao, L., Xia, F., Ding, S., Huang, L., Xiang, H., Wang, J., Xiao, Y., Xiao, R., Huang, J."Ganoderma lucidum polysaccharides protect fibroblasts against UVB-induced photoaging". Molecular Medicine Reports 15, no. 1 (2017): 111-116. https://doi.org/10.3892/mmr.2016.6026
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