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Article

Alanine and arginine rich domain containing protein, Aard, is directly regulated by androgen receptor in mouse Sertoli cells

  • Authors:
    • Qiang Geng
    • Li‑Wei Ni
    • Bin Ouyang
    • Yan‑Hua Hu
    • Yu Zhao
    • Jun Guo
  • View Affiliations / Copyright

    Affiliations: First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, P.R. China, Union Stem Cell & Gene Engineering Co., Ltd, Tianjin 300384, P.R. China, Graduate School of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, P.R. China, Department of Andrology, Xiyuan Hospital of China Academy of Chinese Medical Science, Beijing 100091, P.R. China
  • Pages: 352-358
    |
    Published online on: December 12, 2016
       https://doi.org/10.3892/mmr.2016.6028
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Abstract

Alanine and arginine rich domain containing protein (Aard) is specifically expressed in Sertoli cells (SCs) of mouse testis and the expression increases in an age‑dependent manner. A number of previous studies have indicated that androgen and androgen receptor (AR) signaling pathways are particularly important for spermatogenesis in mouse SCs, however, the association between Aard and AR remain to be elucidated. The present study identified Aard as a gene that is directly regulated by AR in mouse SCs, which is important in spermatogenesis. The expression of AARD was significantly downregulated in the testes of Sertoli cell‑selective AR knockout mice compared with wild‑type mice as analyzed by western blotting and immunofluorescence analyses. Quantitative polymerase chain reaction and western blotting indicated that AARD was predominantly expressed in adult mouse testis and its expression was increased in an age-dependent manner. In addition, AARD expression was upregulated by testosterone in primary SCs in vitro, which was confirmed by bioinformatics analysis and a dual‑luciferase reporter assay. Finally, chromatin immunoprecipitation and electrophoretic mobility shift assays indicated that the ligand‑bound AR activated Aard transcription via directly binding to the androgen‑responsive element of the Aard promoter. To the best of our knowledge, the present study is the first to document that Aard is directly regulated by AR in mouse Sertoli cells.
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Copy and paste a formatted citation
Spandidos Publications style
Geng Q, Ni LW, Ouyang B, Hu YH, Zhao Y and Guo J: Alanine and arginine rich domain containing protein, Aard, is directly regulated by androgen receptor in mouse Sertoli cells. Mol Med Rep 15: 352-358, 2017.
APA
Geng, Q., Ni, L., Ouyang, B., Hu, Y., Zhao, Y., & Guo, J. (2017). Alanine and arginine rich domain containing protein, Aard, is directly regulated by androgen receptor in mouse Sertoli cells. Molecular Medicine Reports, 15, 352-358. https://doi.org/10.3892/mmr.2016.6028
MLA
Geng, Q., Ni, L., Ouyang, B., Hu, Y., Zhao, Y., Guo, J."Alanine and arginine rich domain containing protein, Aard, is directly regulated by androgen receptor in mouse Sertoli cells". Molecular Medicine Reports 15.1 (2017): 352-358.
Chicago
Geng, Q., Ni, L., Ouyang, B., Hu, Y., Zhao, Y., Guo, J."Alanine and arginine rich domain containing protein, Aard, is directly regulated by androgen receptor in mouse Sertoli cells". Molecular Medicine Reports 15, no. 1 (2017): 352-358. https://doi.org/10.3892/mmr.2016.6028
Copy and paste a formatted citation
x
Spandidos Publications style
Geng Q, Ni LW, Ouyang B, Hu YH, Zhao Y and Guo J: Alanine and arginine rich domain containing protein, Aard, is directly regulated by androgen receptor in mouse Sertoli cells. Mol Med Rep 15: 352-358, 2017.
APA
Geng, Q., Ni, L., Ouyang, B., Hu, Y., Zhao, Y., & Guo, J. (2017). Alanine and arginine rich domain containing protein, Aard, is directly regulated by androgen receptor in mouse Sertoli cells. Molecular Medicine Reports, 15, 352-358. https://doi.org/10.3892/mmr.2016.6028
MLA
Geng, Q., Ni, L., Ouyang, B., Hu, Y., Zhao, Y., Guo, J."Alanine and arginine rich domain containing protein, Aard, is directly regulated by androgen receptor in mouse Sertoli cells". Molecular Medicine Reports 15.1 (2017): 352-358.
Chicago
Geng, Q., Ni, L., Ouyang, B., Hu, Y., Zhao, Y., Guo, J."Alanine and arginine rich domain containing protein, Aard, is directly regulated by androgen receptor in mouse Sertoli cells". Molecular Medicine Reports 15, no. 1 (2017): 352-358. https://doi.org/10.3892/mmr.2016.6028
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