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Midazolam anesthesia protects neuronal cells from oxidative stress-induced death via activation of the JNK-ERK pathway

  • Authors:
    • Jing‑Yu Liu
    • Feng Guo
    • Hong‑Ling Wu
    • Ying Wang
    • Jin‑Shan Liu
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, Dongying People's Hospital, Dongying, Shandong 257091, P.R. China, Department of Anesthesiology, Dongying District People's Hospital, Dongying, Shandong 257091, P.R. China, Department of Anesthesiology, Kenli People's Hospital, Dongying, Shandong 257091, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 169-179
    |
    Published online on: December 12, 2016
       https://doi.org/10.3892/mmr.2016.6031
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Abstract

Midazolam is an anesthetic agent commonly used during clinical and surgical procedures, which has been shown to exert ROS‑suppressing and apoptosis‑modulating pharmacological activities in various cellular systems. However, the effects of midazolam on oxidative stress in neuronal cells require elucidation. The present study investigated the effects of midazolam on buthionine sulfoximine (BSO)‑ and hydrogen peroxide (H2O2)‑induced oxidative stress in primary cortical neuronal cells. In addition, the effects of midazolam on middle cerebral artery occlusion (MCAO) in mice and on ethanol‑induced neuroapoptosis in the brains of neonatal mice were determined. Subsequently, cell viability was detected using the MTT assay; intracellular reactive oxygen species (ROS) generation was determined using the 2',7'‑dichlorodihydrofluorescein diacetate method with confocal microscopy; terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was conducted to detect apoptotic cells; immunohistochemistry was performed to detect activated caspase‑3; neuronal deficit and infarct volume analyses were conducted; and quantitative polymerase chain reaction and western blotting were performed to detect the expression levels of genes and proteins associated with apoptosis and cell survival pathways. The results demonstrated that BSO (10 mM) and H2O2 (1 mM) suppressed proliferation of cortical neuronal cells by inducing apoptosis. These effects were suppressed following treatment with midazolam in a dose‑dependent manner. In addition, BSO and H2O2 induced ROS generation in neuronal cells; however, this was effectively suppressed by midazolam (100 µM). Beneficial synergistic effects were detected when midazolam was used in combination with the known antioxidant trolox. BSO and H2O2 also suppressed the protein expression levels of c‑Jun N‑terminal kinases (JNK), phosphorylated (p)JNK, extracellular signal‑regulated kinases (ERK)1/2, pERK1/2, AKT and nuclear factor‑κB; however, expression was recovered following treatment with midazolam. Midazolam also activated protein kinase C‑ε, which was suppressed by BSO, in cortical neuronal cells. In MCAO mice, midazolam post‑conditioning significantly suppressed infarct size and reduced the number of TUNEL‑positive cells. In addition, the expression levels of caspase‑3 and poly (ADP‑ribose) polymerase were suppressed in a dose‑dependent manner. In neonatal mice, midazolam reduced ethanol‑induced activated caspase‑3 staining and apoptotic TUNEL staining. The results of the present study demonstrated that midazolam may protect against neuronal degeneration and neuroapoptosis induced by physiological and oxidative stress.
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Copy and paste a formatted citation
Spandidos Publications style
Liu JY, Guo F, Wu HL, Wang Y and Liu JS: Midazolam anesthesia protects neuronal cells from oxidative stress-induced death via activation of the JNK-ERK pathway. Mol Med Rep 15: 169-179, 2017.
APA
Liu, J., Guo, F., Wu, H., Wang, Y., & Liu, J. (2017). Midazolam anesthesia protects neuronal cells from oxidative stress-induced death via activation of the JNK-ERK pathway. Molecular Medicine Reports, 15, 169-179. https://doi.org/10.3892/mmr.2016.6031
MLA
Liu, J., Guo, F., Wu, H., Wang, Y., Liu, J."Midazolam anesthesia protects neuronal cells from oxidative stress-induced death via activation of the JNK-ERK pathway". Molecular Medicine Reports 15.1 (2017): 169-179.
Chicago
Liu, J., Guo, F., Wu, H., Wang, Y., Liu, J."Midazolam anesthesia protects neuronal cells from oxidative stress-induced death via activation of the JNK-ERK pathway". Molecular Medicine Reports 15, no. 1 (2017): 169-179. https://doi.org/10.3892/mmr.2016.6031
Copy and paste a formatted citation
x
Spandidos Publications style
Liu JY, Guo F, Wu HL, Wang Y and Liu JS: Midazolam anesthesia protects neuronal cells from oxidative stress-induced death via activation of the JNK-ERK pathway. Mol Med Rep 15: 169-179, 2017.
APA
Liu, J., Guo, F., Wu, H., Wang, Y., & Liu, J. (2017). Midazolam anesthesia protects neuronal cells from oxidative stress-induced death via activation of the JNK-ERK pathway. Molecular Medicine Reports, 15, 169-179. https://doi.org/10.3892/mmr.2016.6031
MLA
Liu, J., Guo, F., Wu, H., Wang, Y., Liu, J."Midazolam anesthesia protects neuronal cells from oxidative stress-induced death via activation of the JNK-ERK pathway". Molecular Medicine Reports 15.1 (2017): 169-179.
Chicago
Liu, J., Guo, F., Wu, H., Wang, Y., Liu, J."Midazolam anesthesia protects neuronal cells from oxidative stress-induced death via activation of the JNK-ERK pathway". Molecular Medicine Reports 15, no. 1 (2017): 169-179. https://doi.org/10.3892/mmr.2016.6031
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