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Article

Three-dimensional simulated microgravity culture improves the proliferation and odontogenic differentiation of dental pulp stem cell in PLGA scaffolds implanted in mice

  • Authors:
    • Yanping Li
    • Lina He
    • Shuang Pan
    • Lin Zhang
    • Weiwei Zhang
    • Hong Yi
    • Yumei Niu
  • View Affiliations / Copyright

    Affiliations: Department of Endodontics, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China
  • Pages: 873-878
    |
    Published online on: December 14, 2016
       https://doi.org/10.3892/mmr.2016.6042
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Abstract

Tooth regeneration through stem cell-based therapy is a promising treatment for tooth decay and loss. Human dental pulp stem cells (hDPSCs) have been widely identified as the stem cells with the most potential for tooth tissue regeneration. However, the culture of hDPSCs in vitro for tissue engineering is challenging, as cells may proliferate slowly or/and differentiate poorly in vivo. Dynamic three‑dimensional (3D) simulated microgravity (SMG) created using the rotary cell culture system is considered to an effective tool, which contributes to several cell functions. Thus, the present study aimed to investigate the effect of dynamic 3D SMG culture on the proliferation and odontogenic differentiation abilities of hDPSCs in poly (lactic‑co‑glycolic acid) (PLGA) scaffolds in nude mice. The hDPSCs on PLGA scaffolds were maintained separately in the 3D SMG culture system and static 3D cultures with osteogenic medium for 7 days in vitro. Subsequently, the cell‑PLGA complexes were implanted subcutaneously on the backs of nude mice for 4 weeks. The results of histological and immunohistochemical examinations of Ki‑67, type I collagen, dentin sialoprotein and DMP‑1 indicated that the proliferation and odontogenic differentiation abilities of the hDPSCs prepared in the 3D SMG culture system were higher, compared with those prepared in the static culture system. These findings suggested that dynamic 3D SMG culture likely contributes to tissue engineering by improving the proliferation and odontogenic differentiation abilities of hDPSCs in vivo.
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Copy and paste a formatted citation
Spandidos Publications style
Li Y, He L, Pan S, Zhang L, Zhang W, Yi H and Niu Y: Three-dimensional simulated microgravity culture improves the proliferation and odontogenic differentiation of dental pulp stem cell in PLGA scaffolds implanted in mice. Mol Med Rep 15: 873-878, 2017.
APA
Li, Y., He, L., Pan, S., Zhang, L., Zhang, W., Yi, H., & Niu, Y. (2017). Three-dimensional simulated microgravity culture improves the proliferation and odontogenic differentiation of dental pulp stem cell in PLGA scaffolds implanted in mice. Molecular Medicine Reports, 15, 873-878. https://doi.org/10.3892/mmr.2016.6042
MLA
Li, Y., He, L., Pan, S., Zhang, L., Zhang, W., Yi, H., Niu, Y."Three-dimensional simulated microgravity culture improves the proliferation and odontogenic differentiation of dental pulp stem cell in PLGA scaffolds implanted in mice". Molecular Medicine Reports 15.2 (2017): 873-878.
Chicago
Li, Y., He, L., Pan, S., Zhang, L., Zhang, W., Yi, H., Niu, Y."Three-dimensional simulated microgravity culture improves the proliferation and odontogenic differentiation of dental pulp stem cell in PLGA scaffolds implanted in mice". Molecular Medicine Reports 15, no. 2 (2017): 873-878. https://doi.org/10.3892/mmr.2016.6042
Copy and paste a formatted citation
x
Spandidos Publications style
Li Y, He L, Pan S, Zhang L, Zhang W, Yi H and Niu Y: Three-dimensional simulated microgravity culture improves the proliferation and odontogenic differentiation of dental pulp stem cell in PLGA scaffolds implanted in mice. Mol Med Rep 15: 873-878, 2017.
APA
Li, Y., He, L., Pan, S., Zhang, L., Zhang, W., Yi, H., & Niu, Y. (2017). Three-dimensional simulated microgravity culture improves the proliferation and odontogenic differentiation of dental pulp stem cell in PLGA scaffolds implanted in mice. Molecular Medicine Reports, 15, 873-878. https://doi.org/10.3892/mmr.2016.6042
MLA
Li, Y., He, L., Pan, S., Zhang, L., Zhang, W., Yi, H., Niu, Y."Three-dimensional simulated microgravity culture improves the proliferation and odontogenic differentiation of dental pulp stem cell in PLGA scaffolds implanted in mice". Molecular Medicine Reports 15.2 (2017): 873-878.
Chicago
Li, Y., He, L., Pan, S., Zhang, L., Zhang, W., Yi, H., Niu, Y."Three-dimensional simulated microgravity culture improves the proliferation and odontogenic differentiation of dental pulp stem cell in PLGA scaffolds implanted in mice". Molecular Medicine Reports 15, no. 2 (2017): 873-878. https://doi.org/10.3892/mmr.2016.6042
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