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Article Open Access

Salvianolic acid B reverses multidrug resistance in HCT‑8/VCR human colorectal cancer cells by increasing ROS levels

  • Authors:
    • Piaoting Guo
    • Songpo Wang
    • Wei Liang
    • Wenjing Wang
    • Huijun Wang
    • Miaomiao Zhao
    • Xiaowei Liu
  • View Affiliations / Copyright

    Affiliations: Department of Traditional Chinese Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200240, P.R. China, Department of Gynecology, Shuguang Hospital Affiliated with Shanghai University of T.C.M, Shanghai 200240, P.R. China
    Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 724-730
    |
    Published online on: December 14, 2016
       https://doi.org/10.3892/mmr.2016.6049
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Abstract

Salvianolic acid B (SalB) a water‑soluble phenolic compound, extracted from Salvia miltiorrhiza, has previously been demonstrated to reverse tumor multidrug resistance (MDR), including in colorectal cancer. Reactive oxygen species (ROS) are oxygen radicals generated during aerobic metabolism (superoxide and hydroxyl radicals) and superoxide easily generating free radicals (H2O2). The concept that increased ROS levels can lead to augmented tumor cell‑sensitivity to chemotherapy drugs has become notable. The aim of the present study was to elucidate the role of ROS in mediating the effect of SalB on drug resistance and the correlation with drug resistance‑associated protein, P‑glycoprotein (P‑gp), and apoptosis‑associated proteins, B‑cell lymphoma 2 (Bcl‑2) and Bcl‑2‑associated X (Bax). In the current study, through utilizing the multidrug resistant colorectal cancer cell line, HCT‑8/VCR, it was demonstrate that SalB reversed MDR in HCT‑8/VCR. In addition, SalB significantly increased ROS levels, which may have accelerated the apoptosis of HCT‑8/VCR cells by downregulating Bcl‑2 and increasing Bax protein expression. Furthermore the increased intracellular ROS levels may have inhibited P‑gp expression at the gene and protein levels. In conclusion, the data of the current study demonstrate that SalB reversed MDR in HCT‑8/VCR cells, and the effect is associated with increased ROS levels, which may downregulate P‑gp expression and promote tumor cell apoptosis, which in turn increases the sensitivity of drug‑resistant cells to chemotherapy drugs.
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1 

Siegel RL, Miller KD and Jemal A: Cancer statistics, 2016. CA Cancer J Clin. 66:7–30. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Chen W, Zheng R, Baade PD, Zhang S, Zeng H, Bray F, Jemal A, Yu XQ and He J: Cancer statistics in China, 2015. CA Cancer J Clin. 66:115–132. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Maiti AK: Genetic determinants of oxidative stress-mediated sensitization of drug-resistant cancer cells. Int J Cancer. 130:1–9. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Dahdouh F, Raane M, Thévenod F and Lee WK: Nickel-induced cell death and survival pathways in cultured renal proximal tubule cells: Roles of reactive oxygen species, ceramide and ABCB1. Arch Toxicol. 88:881–892. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Lo YL and Tu WC: Co-encapsulation of chrysophsin-1 and epirubicin in PEGylated liposomes circumvents multidrug resistance in HeLa cells. Chem Biol Interact. 242:13–23. 2015. View Article : Google Scholar : PubMed/NCBI

6 

Brahimi-Horn MC, Giuliano S, Saland E, Lacas-Gervais S, Sheiko T, Pelletier J, Bourget I, Bost F, Féral C, Boulter E, et al: Knockout of Vdac1 activates hypoxia-inducible factor through reactive oxygen species generation and induces tumor growth by promoting metabolic reprogramming and inflammation. Cancer Metab. 3:82015. View Article : Google Scholar : PubMed/NCBI

7 

Singh KK: Mitochondrial dysfunction is a common phenotype in aging and cancer. Ann N Y Acad Sci. 1019:260–264. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Sinicrope FA, Hart J, Brasitus TA, Michelassi F, Lee JJ and Safa AR: Relationship of P-glycoprotein and carcinombryonic antigen expression in human colon carcinoma to local invasion. DNA ploidy, and disease relapse. Caner. 74:2908–2917. 1994. View Article : Google Scholar

9 

Salphati L, Plise EG and Li G: Expression and activity of the efflux transporters ABCB1, ABCC2 and ABCG2 in the human colorectal carcinoma cell line LS513. Eur J Pharm Sci. 37:463–468. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Wang M, Sun G, Wu P, Chen R, Yao F, Qin M, Luo Y, Sun H, Zhang Q, Dong X and Sun X: Salvianolic Acid B prevents arsenic trioxide-induced cardiotoxicity in vivo and enhances its anticancer activity in vitro. Evid Based Complement Alternat Med. 2013:7594832013.PubMed/NCBI

11 

Zhao Y, Hao Y, Ji H, Fang Y, Guo Y, Sha W, Zhou Y, Pang X, Southerland WM, Califano JA and Gu X: Combination effects of salvianolic acid B with low-dose celecoxib on inhibition of head and neck squamous cell carcinoma growth in vitro and in vivo. Cancer Prev Res (Phila). 3:787–796. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

13 

Shen A, Chen H, Chen Y, Lin J, Lin W, Liu L, Sferra TJ and Peng J: Pien Tze Huang Overcomes Multidrug Resistance and Epithelial-Mesenchymal Transition in Human Colorectal Carcinoma Cells via Suppression of TGF-β Pathway. Evid Based Complement Alternat Med. 2014:6794362014. View Article : Google Scholar : PubMed/NCBI

14 

Ravindranath AK, Kaur S, Wernyj RP, Kumaran MN, Miletti-Gonzalez KE, Chan R, Lim E, Madura K and Rodriguez-Rodriguez L: CD44 promotes multi-drug resistance by protecting P-glycoprotein from FBXO21-mediated ubiquitination. Oncotarget. 6:26308–26321. 2015. View Article : Google Scholar : PubMed/NCBI

15 

Katayama K, Noguchi K and Sugimoto Y: FBXO15 regulates P-glycoprotein/ABCB1 expression through the ubiquitin-proteasome pathway in cancer cells. Cancer Sci. 104:694–702. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Sui H, Liu X, Jin BH, Pan SF, Zhou LH, Yu NA, Wu J, Cai JF, Fan ZZ, Zhu HR and Li Q: Zuo Jin Wan, a Traditional Chinese Herbal Formula, Reverses P-gp-Mediated MDR In Vitro and In Vivo. Evid Based Complement Alternat Med. 2013:9570782013. View Article : Google Scholar : PubMed/NCBI

17 

Sun H, Liu XD, Liu Q, Wang FP, Bao XQ and Zhang D: Reversal of P-glycoprotein-mediated multidrug resistance by the novel tetrandrine derivative W6. J Asian Nat Prod Res. 17:638–648. 2015. View Article : Google Scholar : PubMed/NCBI

18 

Jin Y, Zhang Z, Zhao T, Liu X and Jian L: Mixed micelles of doxorubicin overcome multidrug resistance by inhibiting the expression of P-Glycoprotein. J Biomed Nanotechnol. 11:1330–1338. 2015. View Article : Google Scholar : PubMed/NCBI

19 

Beeran AA, Maliyakkal N, Rao CM and Udupa N: The enriched fraction of Elephantopus scaber Triggers apoptosis and inhibits multi-drug resistance transporters in human epithelial cancer cells. Pharmacogn Mag. 11:257–268. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Agarwal A, Ayaz A, Samanta L, Sharma R, Assidi M, Abuzenadah AM and Sabanegh E: Comparative proteomic network signatures in seminal plasma of infertile men as a function of reactive oxygen species. Clin Proteomics. 12:232015. View Article : Google Scholar : PubMed/NCBI

21 

Lo YL, Wang W and Ho CT: 7,3′,4′-Trihydroxyisoflavone modulates multidrug resistance transporters and induces apoptosis via production of reactive oxygen species. Toxicology. 302:221–232. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Pandey V, Chaube B and Bhat MK: Hyperglycemia regulates MDR-1, drug accumulation and ROS levels causing increased toxicity of carboplatin and 5-fluorouracil in MCF-7 cells. J Cell Biochem. 112:2942–2952. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Wei N, Liu GT, Chen XG, Liu Q, Wang FP and Sun H: H1, a derivative of Tetrandrine, exerts anti-MDR activity by initiating intrinsic apoptosis pathway and inhibiting the activation of Erk1/2 and Akt1/2. Biochem Pharmacol. 82:1593–1603. 2011. View Article : Google Scholar : PubMed/NCBI

24 

Singer E, Judkins J, Salomonis N, Matlaf L, Soteropoulos P, McAllister S and Soroceanu L: Reactive oxygen species-mediated therapeutic response and resistance in glioblastoma. Cell Death Dis. 6:e16012015. View Article : Google Scholar : PubMed/NCBI

25 

Brozovic A, Ambriović-Ristov A and Osmak M: The relationship between cisplatin-induced reactive oxygen species, glutathione, and BCL-2 and resistance to cisplatin. Crit Rev Toxicol. 40:347–359. 2010. View Article : Google Scholar : PubMed/NCBI

26 

Okon IS and Zou MH: Mitochondrial ROS and cancer drug resistance: Implications for therapy. Pharmacol Res. 100:170–174. 2015. View Article : Google Scholar : PubMed/NCBI

27 

Okon IS, Coughlan KA, Zhang M, Wang Q and Zou MH: Gefitinib-mediated reactive oxygen specie (ROS) instigates mitochondrial dysfunction and drug resistance in lung cancer cells. J Biol Chem. 290:9101–9110. 2015. View Article : Google Scholar : PubMed/NCBI

28 

Su Z, Chen M, Xiao Y, Sun M, Zong L, Asghar S, Dong M, Li H, Ping Q and Zhang C: ROS-triggered and regenerating anticancer nanosystem: An effective strategy to subdue tumor's multidrug resistance. J Control Release. 196:370–383. 2014. View Article : Google Scholar : PubMed/NCBI

29 

Hao Y, Lin C, Fan H, Yu Y, Li N and Chen S: Proteomic analysis of Cucumis sativus cotyledons after glucohexaose treatment as a part of ROS accumulation related resistance mechanism. Proteome Sci. 12:342014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Guo P, Wang S, Liang W, Wang W, Wang H, Zhao M and Liu X: Salvianolic acid B reverses multidrug resistance in HCT‑8/VCR human colorectal cancer cells by increasing ROS levels. Mol Med Rep 15: 724-730, 2017.
APA
Guo, P., Wang, S., Liang, W., Wang, W., Wang, H., Zhao, M., & Liu, X. (2017). Salvianolic acid B reverses multidrug resistance in HCT‑8/VCR human colorectal cancer cells by increasing ROS levels. Molecular Medicine Reports, 15, 724-730. https://doi.org/10.3892/mmr.2016.6049
MLA
Guo, P., Wang, S., Liang, W., Wang, W., Wang, H., Zhao, M., Liu, X."Salvianolic acid B reverses multidrug resistance in HCT‑8/VCR human colorectal cancer cells by increasing ROS levels". Molecular Medicine Reports 15.2 (2017): 724-730.
Chicago
Guo, P., Wang, S., Liang, W., Wang, W., Wang, H., Zhao, M., Liu, X."Salvianolic acid B reverses multidrug resistance in HCT‑8/VCR human colorectal cancer cells by increasing ROS levels". Molecular Medicine Reports 15, no. 2 (2017): 724-730. https://doi.org/10.3892/mmr.2016.6049
Copy and paste a formatted citation
x
Spandidos Publications style
Guo P, Wang S, Liang W, Wang W, Wang H, Zhao M and Liu X: Salvianolic acid B reverses multidrug resistance in HCT‑8/VCR human colorectal cancer cells by increasing ROS levels. Mol Med Rep 15: 724-730, 2017.
APA
Guo, P., Wang, S., Liang, W., Wang, W., Wang, H., Zhao, M., & Liu, X. (2017). Salvianolic acid B reverses multidrug resistance in HCT‑8/VCR human colorectal cancer cells by increasing ROS levels. Molecular Medicine Reports, 15, 724-730. https://doi.org/10.3892/mmr.2016.6049
MLA
Guo, P., Wang, S., Liang, W., Wang, W., Wang, H., Zhao, M., Liu, X."Salvianolic acid B reverses multidrug resistance in HCT‑8/VCR human colorectal cancer cells by increasing ROS levels". Molecular Medicine Reports 15.2 (2017): 724-730.
Chicago
Guo, P., Wang, S., Liang, W., Wang, W., Wang, H., Zhao, M., Liu, X."Salvianolic acid B reverses multidrug resistance in HCT‑8/VCR human colorectal cancer cells by increasing ROS levels". Molecular Medicine Reports 15, no. 2 (2017): 724-730. https://doi.org/10.3892/mmr.2016.6049
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