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Article

Modulation of low shear stress‑induced eNOS multi‑site phosphorylation and nitric oxide production via protein kinase and ERK1/2 signaling

  • Authors:
    • Xiangquan Kong
    • Xinliang Qu
    • Bing Li
    • Zhimei Wang
    • Yuelin Chao
    • Xiaomin Jiang
    • Wen Wu
    • Shao‑Liang Chen
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu 210006, P.R. China
  • Pages: 908-914
    |
    Published online on: December 20, 2016
       https://doi.org/10.3892/mmr.2016.6060
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Abstract

Physiological shear stress has been demonstrated to serve an atheroprotective function by stimulating endothelial nitric oxide synthase (eNOS) multi‑site phosphorylation. Low shear stress (LSS) serves an atheroprone role by increasing endothelial cell apoptosis and inflammation. The present study assessed whether LSS inhibited nitric oxide (NO) production in human umbilical vein endothelial cells by modulating eNOS phosphorylation and potential signaling pathways. A parallel flow chamber imposed with 2 dyn/cm2 shear stress on endothelial cells was used. Western blotting and 4,5‑diaminofluorescein diacetate were used to analyze the protein expression levels and NO production. LSS activated eNOS‑Ser1177 and eNOS‑Thr495, but inhibited eNOS‑Ser633. NO production was decreased after a transient increase at 5 min. LSS‑stimulated phosphorylation of eNOS‑Ser1177 and ‑Thr495 were suppressed by the Akt inhibitor, perifosine, and extracellular signal regulated kinases1/2 (ERK1/2) inhibitor, PD98059, respectively. Additionally, the phosphorylation of eNOS‑Ser633 inhibited by LSS was restored by the protein kinase A activator, 8‑Bromo‑cAMP. PD98059 completely inhibited the LSS‑induced downregulation of NO production. NO downregulation in response to LSS was intensified by perifosine and was partly inhibited by 8‑Bromo‑cAMP. These results indicated that LSS‑induced activation of ERK1/2/eNOS‑Thr495 serves a major role in inhibiting endothelial NO synthase, which may explain the proinflammatory and proatherosclerotic properties of LSS.
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Copy and paste a formatted citation
Spandidos Publications style
Kong X, Qu X, Li B, Wang Z, Chao Y, Jiang X, Wu W and Chen SL: Modulation of low shear stress‑induced eNOS multi‑site phosphorylation and nitric oxide production via protein kinase and ERK1/2 signaling. Mol Med Rep 15: 908-914, 2017.
APA
Kong, X., Qu, X., Li, B., Wang, Z., Chao, Y., Jiang, X. ... Chen, S. (2017). Modulation of low shear stress‑induced eNOS multi‑site phosphorylation and nitric oxide production via protein kinase and ERK1/2 signaling. Molecular Medicine Reports, 15, 908-914. https://doi.org/10.3892/mmr.2016.6060
MLA
Kong, X., Qu, X., Li, B., Wang, Z., Chao, Y., Jiang, X., Wu, W., Chen, S."Modulation of low shear stress‑induced eNOS multi‑site phosphorylation and nitric oxide production via protein kinase and ERK1/2 signaling". Molecular Medicine Reports 15.2 (2017): 908-914.
Chicago
Kong, X., Qu, X., Li, B., Wang, Z., Chao, Y., Jiang, X., Wu, W., Chen, S."Modulation of low shear stress‑induced eNOS multi‑site phosphorylation and nitric oxide production via protein kinase and ERK1/2 signaling". Molecular Medicine Reports 15, no. 2 (2017): 908-914. https://doi.org/10.3892/mmr.2016.6060
Copy and paste a formatted citation
x
Spandidos Publications style
Kong X, Qu X, Li B, Wang Z, Chao Y, Jiang X, Wu W and Chen SL: Modulation of low shear stress‑induced eNOS multi‑site phosphorylation and nitric oxide production via protein kinase and ERK1/2 signaling. Mol Med Rep 15: 908-914, 2017.
APA
Kong, X., Qu, X., Li, B., Wang, Z., Chao, Y., Jiang, X. ... Chen, S. (2017). Modulation of low shear stress‑induced eNOS multi‑site phosphorylation and nitric oxide production via protein kinase and ERK1/2 signaling. Molecular Medicine Reports, 15, 908-914. https://doi.org/10.3892/mmr.2016.6060
MLA
Kong, X., Qu, X., Li, B., Wang, Z., Chao, Y., Jiang, X., Wu, W., Chen, S."Modulation of low shear stress‑induced eNOS multi‑site phosphorylation and nitric oxide production via protein kinase and ERK1/2 signaling". Molecular Medicine Reports 15.2 (2017): 908-914.
Chicago
Kong, X., Qu, X., Li, B., Wang, Z., Chao, Y., Jiang, X., Wu, W., Chen, S."Modulation of low shear stress‑induced eNOS multi‑site phosphorylation and nitric oxide production via protein kinase and ERK1/2 signaling". Molecular Medicine Reports 15, no. 2 (2017): 908-914. https://doi.org/10.3892/mmr.2016.6060
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