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Article

Influence of microRNA‑34a on proliferation, invasion and metastasis of HCT116 cells

  • Authors:
    • Chunyan Li
    • Shuming Lu
    • Yingyan Wang
    • Shibin Guo
    • Tianyu Zhao
    • Xiaosheng Wang
    • Bo Song
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China, Laboratory Center for Diagnostics, Dalian Medical University, Dalian, Liaoning 116044, P.R. China, Department of Pathology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China
  • Pages: 833-838
    |
    Published online on: December 29, 2016
       https://doi.org/10.3892/mmr.2016.6088
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Abstract

The aim of the present study was to investigate the role of microRNA (miR)‑34a expression in the proliferation, invasion and metastasis of colon cancer and its underlying mechanisms. HCT116 cells were cultured in high‑sugar Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal bovine serum and 1000 U/ml penicillin‑streptomycin. Following digestion and resuspension, the cells were used for transfection, expression and western blot analysis. HCT116 cells from miR‑34a transfection, negative control and blank control groups were seeded into a 96‑well plate at a density of 105 cells/ml, and 200 µl complete DMEM was added. The data are presented as the mean ± standard error. A one‑way analysis of variance was performed to compare groups. miR‑34a‑HCT116 cells demonstrated significantly increased expression levels of miR‑34a. The proliferation of HCT116 cells with overexpression of miR‑34a was significantly inhibited to 0.49±0.11 compared with the blank control group (P<0.001). Compared with the blank control and negative control groups, the protein expression levels of B‑cell lymphoma 2 (Bcl‑2) were markedly reduced in the miR‑34a transfected group. Furthermore, the protein expression levels of Bcl‑2‑associated X protein were significantly increased and those of matrix metalloproteinase (MMP)‑2 and MMP‑9 were markedly reduced in the miR‑34a transfected group, MMP‑9 to a greater extent. The present study suggested that overexpression of miR‑34a may inhibit the proliferation, invasion and metastasis of HCT116 cells.
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1 

Adamowicz K and Zaucha R: Evaluation of the impact of cancer treatment on the adoption and consolidation of pro-health attitudes in the field of cancer in treated patients with colon cancer. J Cancer Educ. 2016.(Epub ahead of print). View Article : Google Scholar : PubMed/NCBI

2 

Kotelevets L, Chastre E, Desmaële D and Couvreur P: Nanotechnologies for the treatment of colon cancer: From old drugs to new hope. Int J Pharm. 514:24–40. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Watanabe T, Itabashi M, Shimada Y, Tanaka S, Ito Y, Ajioka Y, Hamaguchi T, Hyodo I, Igarashi M, Ishida H, et al: Japanese Society for Cancer of the Colon and Rectum (JSCCR) guidelines 2010 for the treatment of colorectal cancer. Int J Clin Oncol. 17:1–29. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Krol J, Loedige I and Filipowicz W: The widespread regulation of microRNA biogenesis, function and decay. Nat Rev Gene. 11:597–610. 2010.

5 

Fabian MR, Sonenberg N and Filipowicz W: Regulation of mRNA translation and stability by microRNAs. Annu Rev Biochem. 79:351–379. 2010. View Article : Google Scholar : PubMed/NCBI

6 

Flynt AS and Lai EC: Biological principles of microRNA-mediated regulation: Shared themes amid diversity. Nat Rev Genet. 9:831–842. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Ventura A and Jacks T: MicroRNAs and cancer: Short RNAs go a long way. Cell. 136:586–591. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Calin GA, Liu CG, Sevignani C, Ferracin M, Felli N, Dumitru CD, Shimizu M, Cimmino A, Zupo S, Dono M, et al: MicroRNA profiling reveals distinct signatures in B cell chronic lymphocytic leukemias. Proc Natl Acad Sci USA. 101:11755–11760. 2004. View Article : Google Scholar : PubMed/NCBI

9 

Kastl L, Brown I and Schofield AC: miRNA-34a is associated with docetaxel resistance in human breast cancer cells. Breast Cancer Res Treat. 131:445–454. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Rathod SS, Rani SB, Khan M, Muzumdar D and Shiras A: Tumor suppressive miRNA-34a suppresses cell proliferation and tumor growth of glioma stem cells by targeting Akt and Wnt signaling pathways. FEBS Open Bio. 4:485–495. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Rodriguez-Ubreva J, Ciudad L, van Oevelen C, Parra M and Graf T: C/EBPa-mediated activation of microRNAs 34a and 223 inhibits Lef1 expression to achieve efficient reprogramming into macrophages. Mol Cell Biol. 34:1145–1157. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

13 

Yothers G, O'Connell MJ, Allegra CJ, Kuebler JP, Colangelo LH, Petrelli NJ and Wolmark N: Oxaliplatin as adjuvant therapy for colon cancer: Updated results of NSABP C-07 trial, including survival and subset analyses. J Clin Oncol. 29:3768–3774. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Volinia S, Calin GA, Liu CG, Ambs S, Cimmino A, Petrocca F, Visone R, Iorio M, Roldo C, Ferracin M, et al: A microRNA expression signature of human solid tumors defines cancer gene targets. Proc Natl Acad Sci USA. 103:2257–2261. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Plönes T, Elze M, Kayser G, Pfeifer D, Burger M and Zissel G: mRNA and miRNA analyses in cytologically positive endobronchial ultrasound-guided transbronchial needle aspiration: Implications for molecular staging in lung cancer patients. Cancer Cytopathol. 122:292–298. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Alexandrov PN, Zhao Y, Jones BM, Bhattacharjee S and Lukiw WJ: Expression of the phagocytosis-essential protein TREM2 is down-regulated by an aluminum-induced miRNA-34a in a murine microglial cell line. J Inorg Biochem. 128:267–269. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Castro RE, Ferreira DM, Afonso MB, Borralho PM, Machado MV, Cortez-Pinto H and Rodrigues CM: miR-34a/SIRT1/p53 is suppressed by ursodeoxycholic acid in the rat liver and activated by disease severity in human non-alcoholic fatty liver disease. J Hepatol. 58:119–125. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Wang P, Zou F, Zhang X, Li H, Dulak A, Tomko RJ Jr, Lazo JS, Wang Z, Zhang L and Yu J: microRNA-21 negatively regulates Cdc25A and cell cycle progression in colon cancer cells. Cancer Res. 69:8157–8165. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Sun R, Chen P, Li L, Sun H, Nie X, Liang Y, Yuan F, Pu Y, Bai P, Zhang L and Gao L: A polymorphism rs4705341 in the flanking region of miR-143/145 predicts risk and prognosis of colorectal cancer. Oncotarget. 2016.(Epub ahead of print).

20 

Wei YS, Xiang Y, Liao PH, Wang JL and Peng YF: An rs4705342 T>C polymorphism in the promoter of miR-143/145 is associated with a decreased risk of ischemic stroke. Sci Rep. 6:346202016. View Article : Google Scholar : PubMed/NCBI

21 

Li N, Fu H, Tie Y, Hu Z, Kong W, Wu Y and Zheng X: miR-34a inhibits migration and invasion by down-regulation of c-Met expression in human hepatocellular carcinoma cells. Cancer Lett. 275:44–53. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Gallardo E, Navarro A, Viñolas N, Marrades RM, Diaz T, Gel B, Quera A, Bandres E, Garcia-Foncillas J, Ramirez J and Monzo M: miR-34a as a prognostic marker of relapse in surgically resected non-small-cell lung cancer. Carcinogenesis. 30:1903–1909. 2009. View Article : Google Scholar : PubMed/NCBI

23 

Tazawa H, Tsuchiya N, Izumiya M and Nakagama H: Tumor-suppressive miR-34a induces senescence-like growth arrest through modulation of the E2F pathway in human colon cancer cells. Proc Natl Acad Sci USA. 104:15472–15477. 2007. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Li C, Lu S, Wang Y, Guo S, Zhao T, Wang X and Song B: Influence of microRNA‑34a on proliferation, invasion and metastasis of HCT116 cells. Mol Med Rep 15: 833-838, 2017.
APA
Li, C., Lu, S., Wang, Y., Guo, S., Zhao, T., Wang, X., & Song, B. (2017). Influence of microRNA‑34a on proliferation, invasion and metastasis of HCT116 cells. Molecular Medicine Reports, 15, 833-838. https://doi.org/10.3892/mmr.2016.6088
MLA
Li, C., Lu, S., Wang, Y., Guo, S., Zhao, T., Wang, X., Song, B."Influence of microRNA‑34a on proliferation, invasion and metastasis of HCT116 cells". Molecular Medicine Reports 15.2 (2017): 833-838.
Chicago
Li, C., Lu, S., Wang, Y., Guo, S., Zhao, T., Wang, X., Song, B."Influence of microRNA‑34a on proliferation, invasion and metastasis of HCT116 cells". Molecular Medicine Reports 15, no. 2 (2017): 833-838. https://doi.org/10.3892/mmr.2016.6088
Copy and paste a formatted citation
x
Spandidos Publications style
Li C, Lu S, Wang Y, Guo S, Zhao T, Wang X and Song B: Influence of microRNA‑34a on proliferation, invasion and metastasis of HCT116 cells. Mol Med Rep 15: 833-838, 2017.
APA
Li, C., Lu, S., Wang, Y., Guo, S., Zhao, T., Wang, X., & Song, B. (2017). Influence of microRNA‑34a on proliferation, invasion and metastasis of HCT116 cells. Molecular Medicine Reports, 15, 833-838. https://doi.org/10.3892/mmr.2016.6088
MLA
Li, C., Lu, S., Wang, Y., Guo, S., Zhao, T., Wang, X., Song, B."Influence of microRNA‑34a on proliferation, invasion and metastasis of HCT116 cells". Molecular Medicine Reports 15.2 (2017): 833-838.
Chicago
Li, C., Lu, S., Wang, Y., Guo, S., Zhao, T., Wang, X., Song, B."Influence of microRNA‑34a on proliferation, invasion and metastasis of HCT116 cells". Molecular Medicine Reports 15, no. 2 (2017): 833-838. https://doi.org/10.3892/mmr.2016.6088
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