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Article

Inhibition of TLR4 protects rat islets against lipopolysaccharide-induced dysfunction

Retraction in: /10.3892/mmr.2025.13622
  • Authors:
    • Xiao Wang
    • Qin Min Ge
    • Fan Bian
    • Yan Dong
    • Chun Mei Huang
  • View Affiliations / Copyright

    Affiliations: Department of Emergency, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, P.R. China, Department of Nephrology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, P.R. China, Department of Endocrinology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, P.R. China
  • Pages: 805-812
    |
    Published online on: December 30, 2016
       https://doi.org/10.3892/mmr.2016.6097
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Abstract

Oxidative stress leads to dysfunction in pancreatic cells, causing a reduction in insulin secretion following exposure to glucose. Toll-like receptor 4 (TLR4) may be activated by exposure to lipopolysaccharide (LPS) stress. TLR4 may mediate the initiation of inflammatory and immune defense responses; however, the importance of the LPS/TLR4 interaction in apoptosis induced by oxidative stress in pancreatic β cells remains to be elucidated. The present study aimed to investigate the importance of TLR4 during LPS‑induced oxidative stress, apoptosis and dysfunction of insulin secretion in isolated islets of rats. LPS‑induced stimulation of TLR4 increased the production of reactive oxygen species and promoted apoptosis by upregulating the expression levels of caspase‑3, poly ADP ribose polymerase and altering the expression ratio of B‑cell lymphoma‑2 (Bcl‑2)/Bcl‑2 associated X protein. Additionally, the insulin secretion of islets cells was reduced. Anti‑TLR4 antibody and a knockdown of TLR4 by TLR4‑short hairpin RNA were used to inhibit TLR4 activity, which may reverse LPS‑induced events. The present study determined that in islets exposed to LPS oxidative stress, dysfunction may be partly mediated via the TLR4 pathway. Inhibition of TLR4 may prevent dysfunction of rat islets due to oxidative stress. The present study revealed that targeting the LPS/TLR4 signaling pathway and antioxidant therapy may be a novel treatment for the severely septic patients with hyperglycemia stress.
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1 

Beutler B and Rietschel ET: Innate immune sensing and its roots: The story of endotoxin. Nat Rev Immunol. 3:169–176. 2003. View Article : Google Scholar : PubMed/NCBI

2 

Zahar JR, Timsit JF, Garrouste-Orgeas M, Français A, Vesin A, Descorps-Declere A, Dubois Y, Souweine B, Haouache H, Goldgran-Toledano D, et al: Outcomes in severe sepsis and patients with septic shock: Pathogen species and infection sites are not associated with mortality. Crit Care Med. 39:1886–1895. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Giribabu N, Kumar KE, Rekha SS, Muniandy S and Salleh N: Chlorophytum borivilianum root extract maintains near normal blood glucose, insulin and lipid profile levels and prevents oxidative stress in the pancreas of streptozotocin-induced adult male diabetic rats. Int J Med Sci. 11:1172–1184. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Coskun O, Kanter M, Korkmaz A and Oter S: Quercetin, a flavonoid antioxidant, prevents and protects streptozotocin-induced oxidative stress and beta-cell damage in rat pancreas. Pharmacol Res. 51:117–123. 2005. View Article : Google Scholar : PubMed/NCBI

5 

Du SC, Ge QM, Lin N, Dong Y and Su Q: ROS-mediated lipopolysaccharide-induced apoptosis in INS-1 cells by modulation of Bcl-2 and Bax. Cell Mol Biol (Noisy-le-grand). 58 Suppl:OL1654–OL1659. 2012.PubMed/NCBI

6 

Medzhitov R: Toll-like receptors and innate immunity. Nat Rev Immunol. 1:135–145. 2001. View Article : Google Scholar : PubMed/NCBI

7 

Reyna SM, Ghosh S, Tantiwong P, Meka CS, Eagan P, Jenkinson CP, Cersosimo E, Defronzo RA, Coletta DK, Sriwijitkamol A and Musi N: Elevated toll-like receptor 4 expression and signaling in muscle from insulin-resistant subjects. Diabetes. 57:2595–2602. 2008. View Article : Google Scholar : PubMed/NCBI

8 

Shi H, Kokoeva MV, Inouye K, Tzameli I, Yin H and Flier JS: TLR4 links innate immunity and fatty acid-induced insulin resistance. J Clin Invest. 116:3015–3025. 2006. View Article : Google Scholar : PubMed/NCBI

9 

Li M, Song L, Gao X, Chang W and Qin X: Toll-like receptor 4 on islet beta cells senses expression changes in high-mobility group box 1 and contributes to the initiation of type 1 diabetes. Exp Mol Med. 44:260–267. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Cucak H, Mayer C, Tonnesen M, Thomsen LH, Grunnet LG and Rosendahl A: Macrophage contact dependent and independent TLR4 mechanisms induce β-cell dysfunction and apoptosis in a mouse model of type 2 diabetes. PLoS One. 9:e906852014. View Article : Google Scholar : PubMed/NCBI

11 

Wang Y: Attenuation of berberine on lipopolysaccharide-induced inflammatory and apoptosis responses in β-cells via TLR4-independent JNK/NF-κB pathway. Pharm Biol. Nov 5–2013.(Epub ahead of print).

12 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

13 

Hotamisligil GS: Inflammation and metabolic disorders. Nature. 444:860–867. 2006. View Article : Google Scholar : PubMed/NCBI

14 

Yoneyama S, Terashima H, Yamaguchi R, Tadano S and Ohkohchi N: The manner of the inflammation-boosting effect caused by acute hyperglycemia secondary to overfeeding and the effects of insulin therapy in a rat model of sepsis. J Surg Res. 185:380–387. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Viana MV, Moraes RB, Fabbrin AR, Santos MF and Gerchman F: Assessment and treatment of hyperglycemia in critically ill patients. Rev Bras Ter Intensiva. 26:71–76. 2014.(In Portuguese). View Article : Google Scholar : PubMed/NCBI

16 

Muhl D, Woth G, Drenkovics L, Varga A, Ghosh S, Csontos C, Bogár L, Wéber G and Lantos J: Comparison of oxidative stress & leukocyte activation in patients with severe sepsis & burn injury. Indian J Med Res. 134:69–78. 2011.PubMed/NCBI

17 

Ling PR, Smith RJ and Bistrian BR: Acute effects of hyperglycemia and hyperinsulinemia on hepatic oxidative stress and the systemic inflammatory response in rats. Crit Care Med. 35:555–560. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Henriksnas J, Lau J, Zang G, Berggren PO, Kohler M and Carlsson PO: Markedly decreased blood perfusion of pancreatic islets transplanted intraportally into the liver: Disruption of islet integrity necessary for islet revascularization. Diabetes. 61:665–673. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Zhu HQ, Gao Y, Guo HR, Kong QZ, Ma Y, Wang JZ, Pan SH, Jiang HC and Dai WJ: Pretreatment with bilirubin protects islet against oxidative injury during isolation and purification. Transplant Proc. 43:1810–1814. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Armann B, Hanson MS, Hatch E, Steffen A and Fernandez LA: Quantification of basal and stimulated ROS levels as predictors of islet potency and function. Am J Transplant. 7:38–47. 2007. View Article : Google Scholar : PubMed/NCBI

21 

Duprez J, Roma LP, Close AF and Jonas JC: Protective antioxidant and antiapoptotic effects of ZnCl2 in rat pancreatic islets cultured in low and high glucose concentrations. PLoS One. 7:e468312012. View Article : Google Scholar : PubMed/NCBI

22 

Roger T, Froidevaux C, Le Roy D, Reymond MK, Chanson AL, Mauri D, Burns K, Riederer BM, Akira S and Calandra T: Protection from lethal gram-negative bacterial sepsis by targeting Toll-like receptor 4. Proc Natl Acad Sci USA. 106:2348–2352. 2009. View Article : Google Scholar : PubMed/NCBI

23 

Li Y, Zhou ZG, Xia QJ, Zhang J, Li HG, Cao GQ, Wang R, Lu YL and Hu TZ: Toll-like receptor 4 detected in exocrine pancreas and the change of expression in cerulein-induced pancreatitis. Pancreas. 30:375–381. 2005. View Article : Google Scholar : PubMed/NCBI

24 

Amyot J, Semache M, Ferdaoussi M, Fontés G and Poitout V: Lipopolysaccharides impair insulin gene expression in isolated islets of langerhans via toll-like receptor-4 and NF-κB signalling. PLoS One. 7:e362002012. View Article : Google Scholar : PubMed/NCBI

25 

Apostolova N, Garcia-Bou R, Hernandez-Mijares A, Herance R, Rocha M and Victor VM: Mitochondrial antioxidants alleviate oxidative and nitrosative stress in a cellular model of sepsis. Pharm Res. 28:2910–2919. 2011. View Article : Google Scholar : PubMed/NCBI

26 

Lu H, Koshkin V, Allister EM, Gyulkhandanyan AV and Wheeler MB: Molecular and metabolic evidence for mitochondrial defects associated with beta-cell dysfunction in a mouse model of type 2 diabetes. Diabetes. 59:448–459. 2010. View Article : Google Scholar : PubMed/NCBI

27 

Vivot K, Langlois A, Bietiger W, Dal S, Seyfritz E, Pinget M, Jeandidier N, Maillard E, Gies JP and Sigrist S: Pro-inflammatory and pro-oxidant status of pancreatic islet in vitro is controlled by TLR-4 and HO-1 pathways. PLoS One. 9:e1076562014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang X, Ge QM, Bian F, Dong Y and Huang CM: Inhibition of TLR4 protects rat islets against lipopolysaccharide-induced dysfunction Retraction in /10.3892/mmr.2025.13622. Mol Med Rep 15: 805-812, 2017.
APA
Wang, X., Ge, Q.M., Bian, F., Dong, Y., & Huang, C.M. (2017). Inhibition of TLR4 protects rat islets against lipopolysaccharide-induced dysfunction Retraction in /10.3892/mmr.2025.13622. Molecular Medicine Reports, 15, 805-812. https://doi.org/10.3892/mmr.2016.6097
MLA
Wang, X., Ge, Q. M., Bian, F., Dong, Y., Huang, C. M."Inhibition of TLR4 protects rat islets against lipopolysaccharide-induced dysfunction Retraction in /10.3892/mmr.2025.13622". Molecular Medicine Reports 15.2 (2017): 805-812.
Chicago
Wang, X., Ge, Q. M., Bian, F., Dong, Y., Huang, C. M."Inhibition of TLR4 protects rat islets against lipopolysaccharide-induced dysfunction Retraction in /10.3892/mmr.2025.13622". Molecular Medicine Reports 15, no. 2 (2017): 805-812. https://doi.org/10.3892/mmr.2016.6097
Copy and paste a formatted citation
x
Spandidos Publications style
Wang X, Ge QM, Bian F, Dong Y and Huang CM: Inhibition of TLR4 protects rat islets against lipopolysaccharide-induced dysfunction Retraction in /10.3892/mmr.2025.13622. Mol Med Rep 15: 805-812, 2017.
APA
Wang, X., Ge, Q.M., Bian, F., Dong, Y., & Huang, C.M. (2017). Inhibition of TLR4 protects rat islets against lipopolysaccharide-induced dysfunction Retraction in /10.3892/mmr.2025.13622. Molecular Medicine Reports, 15, 805-812. https://doi.org/10.3892/mmr.2016.6097
MLA
Wang, X., Ge, Q. M., Bian, F., Dong, Y., Huang, C. M."Inhibition of TLR4 protects rat islets against lipopolysaccharide-induced dysfunction Retraction in /10.3892/mmr.2025.13622". Molecular Medicine Reports 15.2 (2017): 805-812.
Chicago
Wang, X., Ge, Q. M., Bian, F., Dong, Y., Huang, C. M."Inhibition of TLR4 protects rat islets against lipopolysaccharide-induced dysfunction Retraction in /10.3892/mmr.2025.13622". Molecular Medicine Reports 15, no. 2 (2017): 805-812. https://doi.org/10.3892/mmr.2016.6097
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