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The metastasis suppressor gene KISS-1 regulates osteosarcoma apoptosis and autophagy processes

  • Authors:
    • Yiran Yin
    • Lian Tang
    • Lei Shi
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, P.R. China, Department of Gastroenterology, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, P.R. China
    Copyright: © Yin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1286-1290
    |
    Published online on: January 11, 2017
       https://doi.org/10.3892/mmr.2017.6109
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Abstract

The expression of the metastasis suppressor gene KISS-1 in osteosarcoma cells during apoptosis and autophagy was evaluated. MG-63 osteosarcoma cells were transfected with either KISS-1 overexpression or KISS-1 knockdown expression vector in vitro, and compared with cell lines transfected with empty vector. After 12, 24, 48 and 72 h of cell culture, the cell proliferation was examined. The MTT method was used to detect apoptosis by flow cytometry, and the mRNA levels of apoptosis and autophagy markers caspase-3, Bcl-2, Bax, LC3 and Beclin1 were assessed by RT-PCR. Our results showed that cells in the control and low expression group kept proliferating during the cell culture period of 72 h, while the cells in the overexpression group progressively decreased in number. Also, the proliferation rate of the low expression group was significantly higher than that of the control group. The relative mRNA expression levels of caspase-3 and Bax mRNA in the control and low expression group showed no change (the expression was lowest in the low expression group). Moreover, the mRNA level of Bcl-2 increased in both cell groups. The mRNA expression levels of caspase-3 and Bax in the overexpression group were increased, and the level of Bcl-2 was reduced significantly. At the same time, the relative expression level of LC3 and Beclin1 mRNA in the control and low expression groups remained the same, and that of the overexpression group increased. The mRNA levels of LC3 and Beclin1 in the overexpression group were the highest, and that of the low expression group the lowest. The differences were statistically significant (P<0.05). Based on these results, we showed that KISS-1 inhibited the proliferation of osteosarcoma in vitro, probably by accelerating the processes of apoptosis and autophagy in the cells.
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Copy and paste a formatted citation
Spandidos Publications style
Yin Y, Tang L and Shi L: The metastasis suppressor gene KISS-1 regulates osteosarcoma apoptosis and autophagy processes. Mol Med Rep 15: 1286-1290, 2017.
APA
Yin, Y., Tang, L., & Shi, L. (2017). The metastasis suppressor gene KISS-1 regulates osteosarcoma apoptosis and autophagy processes. Molecular Medicine Reports, 15, 1286-1290. https://doi.org/10.3892/mmr.2017.6109
MLA
Yin, Y., Tang, L., Shi, L."The metastasis suppressor gene KISS-1 regulates osteosarcoma apoptosis and autophagy processes". Molecular Medicine Reports 15.3 (2017): 1286-1290.
Chicago
Yin, Y., Tang, L., Shi, L."The metastasis suppressor gene KISS-1 regulates osteosarcoma apoptosis and autophagy processes". Molecular Medicine Reports 15, no. 3 (2017): 1286-1290. https://doi.org/10.3892/mmr.2017.6109
Copy and paste a formatted citation
x
Spandidos Publications style
Yin Y, Tang L and Shi L: The metastasis suppressor gene KISS-1 regulates osteosarcoma apoptosis and autophagy processes. Mol Med Rep 15: 1286-1290, 2017.
APA
Yin, Y., Tang, L., & Shi, L. (2017). The metastasis suppressor gene KISS-1 regulates osteosarcoma apoptosis and autophagy processes. Molecular Medicine Reports, 15, 1286-1290. https://doi.org/10.3892/mmr.2017.6109
MLA
Yin, Y., Tang, L., Shi, L."The metastasis suppressor gene KISS-1 regulates osteosarcoma apoptosis and autophagy processes". Molecular Medicine Reports 15.3 (2017): 1286-1290.
Chicago
Yin, Y., Tang, L., Shi, L."The metastasis suppressor gene KISS-1 regulates osteosarcoma apoptosis and autophagy processes". Molecular Medicine Reports 15, no. 3 (2017): 1286-1290. https://doi.org/10.3892/mmr.2017.6109
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