Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
March-2017 Volume 15 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
March-2017 Volume 15 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Overexpression of GRP75 inhibits inflammation in a rat model of intracerebral hemorrhage

  • Authors:
    • Lian‑Jie Lv
    • Jia Li
    • Hai‑Bo Qiao
    • Ben‑Jin Nie
    • Peng Lu
    • Feng Xue
    • Zhi‑Ming Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, Tianjin Nankai Hospital, Tianjin 300100, P.R. China
  • Pages: 1368-1372
    |
    Published online on: January 17, 2017
       https://doi.org/10.3892/mmr.2017.6126
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Glucose‑regulated protein 75 (GRP75) is a member of the heat shock protein 70 family and previous studies have demonstrated that GRP75 is involved in diseases of the central nervous system. However, the biological function of GRP75 in intracerebral hemorrhage (ICH) remains to be clarified. Thus, the aim of the present study was to evaluate the effects of GRP75 in a rat model of ICH. Western blotting was used to detect the protein expression of GRP75, active caspase‑3, Bax, Bcl‑2, p‑Akt and Akt in brain tissues following ICH. The levels of tumor necrosis factor‑α (TNF‑α) and interleukin (IL)‑1β were evaluated using ELISA assay. Expression of GRP75 mRNA and protein was demonstrated to be reduced in the brain tissues of rats with ICH compared with sham‑operated rats. In addition, overexpression of GRP75 in brain tissues with ICH significantly inhibited the production of the inflammatory cytokines TNF‑α and IL-1β and increased Bcl‑2/decreased Bax levels compared with ICH alone. Furthermore, overexpression of GRP75 in brain tissues with ICH resulted in significantly increased phosphorylation of Akt compared with ICH alone. Therefore, the present study demonstrated, for the first time to the best of our knowledge, significantly reduced GRP75 expression in brain tissues following ICH, and that overexpression of GRP75 inhibits inflammation and potentially inhibits neuronal apoptosis in a rat model of ICH. GRP75 may, therefore, represent a promising target in the treatment of ICH.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Schmidt LB, Geertz S, Wohlfahrt J, Melbye M and Munch TN: Recurrent intracerebral hemorrhage: Associations with comorbidities and medicine with antithrombotic effects. PLoS One. 11:e01662232016. View Article : Google Scholar : PubMed/NCBI

2 

Matsushita K, Meng W, Wang X, Asahi M, Asahi K, Moskowitz MA and Lo EH: Evidence for apoptosis after intracerebral hemorrhage in rat striatum. J Cereb Blood Flow Metab. 20:396–404. 2000. View Article : Google Scholar : PubMed/NCBI

3 

Ran Q, Wadhwa R, Kawai R, Kaul SC, Sifers RN, Bick RJ, Smith JR and Pereira-Smith OM: Extramitochondrial localization of mortalin/mthsp70/PBP74/GRP75. Biochem Biophys Res Commun. 275:174–179. 2000. View Article : Google Scholar : PubMed/NCBI

4 

Wadhwa R, Taira K and Kaul SC: An Hsp70 family chaperone, mortalin/mthsp70/PBP74/Grp75: What, when, and where? Cell Stress Chaperones. 7:309–316. 2002. View Article : Google Scholar : PubMed/NCBI

5 

Zhang G, Han M, Wang X and Xiao A: GRP75 Involves in retinal ganglion cell apoptosis after rat optic nerve crush. J Mol Neurosci. 56:422–430. 2015. View Article : Google Scholar : PubMed/NCBI

6 

Yang L, Liu X, Hao J, Yang Y, Zhao M, Zuo J and Liu W: Glucose-regulated protein 75 suppresses apoptosis induced by glucose deprivation in PC12 cells through inhibition of Bax conformational change. Acta Biochim Biophys Sin (Shanghai). 40:339–348. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Voloboueva LA, Emery JF, Sun X and Giffard RG: Inflammatory response of microglial BV-2 cells includes a glycolytic shift and is modulated by mitochondrial glucose-regulated protein 75/mortalin. FEBS Lett. 587:756–762. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Yang S, Song S, Hua Y, Nakamura T, Keep RF and Xi G: Effects of thrombin on neurogenesis after intracerebral hemorrhage. Stroke. 39:2079–2084. 2008. View Article : Google Scholar : PubMed/NCBI

9 

Livak K and Schmittgen T: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

10 

Tao X, Xie L, Duan C, Dai S, Ren J, Yan Y, Shen J, Lu H and Ge J: Up-regulation of interferon regulatory factor 3 involves in neuronal apoptosis after intracerebral hemorrhage in adult rats. Neurochem Res. 41:2937–2947. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Shih YY, Lee H, Nakagawara A, Juan HF, Jeng YM, Tsay YG, Lin DT, Hsieh FJ, Pan CY, Hsu WM and Liao YF: Nuclear GRP75 binds retinoic acid receptors to promote neuronal differentiation of neuroblastoma. PLoS One. 6:e262362011. View Article : Google Scholar : PubMed/NCBI

12 

Jin J, Hulette C, Wang Y, Zhang T, Pan C, Wadhwa R and Zhang J: Proteomic identification of a stress protein, mortalin/mthsp70/GRP75: Relevance to Parkinson disease. Mol Cell Proteomics. 5:1193–1204. 2006. View Article : Google Scholar : PubMed/NCBI

13 

Liu Ft Chen Y, Yang Yj, Yang L, Yu M, Zhao J, Wu JJ, Huang F, Liu W, Ding ZT and Wang J: Involvement of mortalin/GRP75/mthsp70 in the mitochondrial impairments induced by A53T mutant α-synuclein. Brain Res. 1604:52–61. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Liu Y, Liu W, Song XD and Zuo J: Effect of GRP75/mthsp70/PBP74/mortalin overexpression on intracellular ATP level, mitochondrial membrane potential and ROS accumulation following glucose deprivation in PC12 cells. Mol Cell Biochem. 268:45–51. 2005. View Article : Google Scholar : PubMed/NCBI

15 

Wu J, Yang S, Xi G, Song S, Fu G, Keep RF and Hua Y: Microglial activation and brain injury after intracerebral hemorrhage. Acta Neurochir Suppl. 105:59–65. 2008. View Article : Google Scholar : PubMed/NCBI

16 

Wu S, Fang CX, Kim J and Ren J: Enhanced pulmonary inflammation following experimental intracerebral hemorrhage. Exp Neurol. 200:245–249. 2006. View Article : Google Scholar : PubMed/NCBI

17 

Hirashima Y, Nakamura S, Endo S, Kuwayama N, Naruse Y and Takaku A: Elevation of platelet activating factor, inflammatory cytokines, and coagulation factors in the internal jugular vein of patients with subarachnoid hemorrhage. Neurochem Res. 22:1249–1255. 1997. View Article : Google Scholar : PubMed/NCBI

18 

Abraham E, Bursten S, Shenkar R, Allbee J, Tuder R, Woodson P, Guidot DM, Rice G, Singer JW and Repine JE: Phosphatidic acid signaling mediates lung cytokine expression and lung inflammatory injury after hemorrhage in mice. J Exp Med. 181:569–575. 1995. View Article : Google Scholar : PubMed/NCBI

19 

Tawa P, Hell K, Giroux A, Grimm E, Han Y, Nicholson DW and Xanthoudakis S: Catalytic activity of caspase-3 is required for its degradation: Stabilization of the active complex by synthetic inhibitors. Cell Death Differ. 11:439–447. 2004. View Article : Google Scholar : PubMed/NCBI

20 

Smaili SS, Hsu YT, Sanders KM, Russell JT and Youle RJ: Bax translocation to mitochondria subsequent to a rapid loss of mitochondrial membrane potential. Cell Death Differ. 8:909–920. 2001. View Article : Google Scholar : PubMed/NCBI

21 

Leinninger GM, Backus C, Uhler MD, Lentz SI and Feldman EL: Phosphatidylinositol 3-kinase and Akt effectors mediate insulin-like growth factor-I neuroprotection in dorsal root ganglia neurons. FASEB J. 18:1544–1546. 2004.PubMed/NCBI

22 

Xu X, Chua CC, Gao J, Chua KW, Wang H, Hamdy RC and Chua BH: Neuroprotective effect of humanin on cerebral ischemia/reperfusion injury is mediated by a PI3K/Akt pathway. Brain Res. 1227:12–18. 2008. View Article : Google Scholar : PubMed/NCBI

23 

Brunet A, Datta SR and Greenberg ME: Transcription-dependent and-independent control of neuronal survival by the PI3K-Akt signaling pathway. Curr Opin Neurobiol. 11:297–305. 2001. View Article : Google Scholar : PubMed/NCBI

24 

Romashkova JA and Makarov SS: NF-κB is a target of AKT in anti-apoptotic PDGF signalling. Nature. 401:86–90. 1999. View Article : Google Scholar : PubMed/NCBI

25 

Zheng WH, Kar S and Quirion R: Insulin-like growth factor-1-induced phosphorylation of transcription factor FKHRL1 is mediated by phosphatidylinositol 3-kinase/akt kinase and role of this pathway in insulin-like growth factor-1-induced survival of cultured hippocampal neurons. Mol Pharmacol. 62:225–233. 2002. View Article : Google Scholar : PubMed/NCBI

26 

Zhang X, Chen Y, Jenkins LW, Kochanek PM and Clark RS: Bench-to-bedside review: Apoptosis/programmed cell death triggered by traumatic brain injury. Crit Care. 9:66–75. 2005. View Article : Google Scholar : PubMed/NCBI

27 

Yang L, Guo W, Zhang Q, Li H, Liu X, Yang Y, Zuo J and Liu W: Crosstalk between Raf/MEK/ERK and PI3K/AKT in suppression of Bax conformational change by Grp75 under glucose deprivation conditions. J Mol Biol. 414:654–666. 2011. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Lv LJ, Li J, Qiao HB, Nie BJ, Lu P, Xue F and Zhang ZM: Overexpression of GRP75 inhibits inflammation in a rat model of intracerebral hemorrhage. Mol Med Rep 15: 1368-1372, 2017.
APA
Lv, L., Li, J., Qiao, H., Nie, B., Lu, P., Xue, F., & Zhang, Z. (2017). Overexpression of GRP75 inhibits inflammation in a rat model of intracerebral hemorrhage. Molecular Medicine Reports, 15, 1368-1372. https://doi.org/10.3892/mmr.2017.6126
MLA
Lv, L., Li, J., Qiao, H., Nie, B., Lu, P., Xue, F., Zhang, Z."Overexpression of GRP75 inhibits inflammation in a rat model of intracerebral hemorrhage". Molecular Medicine Reports 15.3 (2017): 1368-1372.
Chicago
Lv, L., Li, J., Qiao, H., Nie, B., Lu, P., Xue, F., Zhang, Z."Overexpression of GRP75 inhibits inflammation in a rat model of intracerebral hemorrhage". Molecular Medicine Reports 15, no. 3 (2017): 1368-1372. https://doi.org/10.3892/mmr.2017.6126
Copy and paste a formatted citation
x
Spandidos Publications style
Lv LJ, Li J, Qiao HB, Nie BJ, Lu P, Xue F and Zhang ZM: Overexpression of GRP75 inhibits inflammation in a rat model of intracerebral hemorrhage. Mol Med Rep 15: 1368-1372, 2017.
APA
Lv, L., Li, J., Qiao, H., Nie, B., Lu, P., Xue, F., & Zhang, Z. (2017). Overexpression of GRP75 inhibits inflammation in a rat model of intracerebral hemorrhage. Molecular Medicine Reports, 15, 1368-1372. https://doi.org/10.3892/mmr.2017.6126
MLA
Lv, L., Li, J., Qiao, H., Nie, B., Lu, P., Xue, F., Zhang, Z."Overexpression of GRP75 inhibits inflammation in a rat model of intracerebral hemorrhage". Molecular Medicine Reports 15.3 (2017): 1368-1372.
Chicago
Lv, L., Li, J., Qiao, H., Nie, B., Lu, P., Xue, F., Zhang, Z."Overexpression of GRP75 inhibits inflammation in a rat model of intracerebral hemorrhage". Molecular Medicine Reports 15, no. 3 (2017): 1368-1372. https://doi.org/10.3892/mmr.2017.6126
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team