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Article Open Access

Interference with Tim-3 protein expression attenuates the invasion of clear cell renal cell carcinoma and aggravates anoikis

Retraction in: /10.3892/mmr.2025.13766
  • Authors:
    • Muming Yu
    • Bin Lu
    • Yancun Liu
    • Ying Me
    • Lijun Wang
    • Hui Li
  • View Affiliations / Copyright

    Affiliations: Emergency Department, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China, Clinical Laboratory, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, P.R. China
    Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1103-1108
    |
    Published online on: January 23, 2017
       https://doi.org/10.3892/mmr.2017.6136
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Abstract

Tumor cells resistant to anoikis are considered to be candidates for metastasis. In the present study, the role of Tim‑3 in anoikis and its influence on the invasion of clear cell renal cell carcinoma (ccRCC) was investigated. Here, polyhydroxylethylmethacrylate (poly‑HEMA) was applied to two ccRCC cell lines, 786‑O and Caki‑2, to induce detachment from the extracellular matrix (ECM). Tim‑3 mRNA and protein expression levels were assayed by reverse transcription-quantitative polymerase chain reaction (RT‑qPCR) and western blot, respectively. Anoikis was measured by Ho33342/PI double staining, acridine orange staining, and further determined using the CytoSelect™ 24‑well Anoikis Assay kit. Apoptosis was measured using flow cytometry, E‑cadherin and N‑cadherin protein expression were determined using western blotting and a Chemicon cell invasion assay kit was used to quantify the invasive capacity of 786‑O and Caki‑2 cells. It was demonstrated that detachment from the ECM decreases transcription and the protein expression level of Tim‑3 in 786‑O and Caki‑2 cells compared with control cells. Interference with Tim‑3 expression using small interfering RNA exacerbated anoikis in 786‑O and Caki‑2 cells induced by poly‑HEMA treatment. E‑cadherin upregulation, N‑cadherin downregulation, and ECM detachment‑induced reduction in invasion ability were all exacerbated by knockdown of Tim‑3. In conclusion, interference with Tim‑3 expression may attenuate the invasion of renal cell carcinoma by aggravating anoikis, indicating Tim‑3 as a potential therapeutic target for treating ccRCC.
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Copy and paste a formatted citation
Spandidos Publications style
Yu M, Lu B, Liu Y, Me Y, Wang L and Li H: Interference with Tim-3 protein expression attenuates the invasion of clear cell renal cell carcinoma and aggravates anoikis Retraction in /10.3892/mmr.2025.13766. Mol Med Rep 15: 1103-1108, 2017.
APA
Yu, M., Lu, B., Liu, Y., Me, Y., Wang, L., & Li, H. (2017). Interference with Tim-3 protein expression attenuates the invasion of clear cell renal cell carcinoma and aggravates anoikis Retraction in /10.3892/mmr.2025.13766. Molecular Medicine Reports, 15, 1103-1108. https://doi.org/10.3892/mmr.2017.6136
MLA
Yu, M., Lu, B., Liu, Y., Me, Y., Wang, L., Li, H."Interference with Tim-3 protein expression attenuates the invasion of clear cell renal cell carcinoma and aggravates anoikis Retraction in /10.3892/mmr.2025.13766". Molecular Medicine Reports 15.3 (2017): 1103-1108.
Chicago
Yu, M., Lu, B., Liu, Y., Me, Y., Wang, L., Li, H."Interference with Tim-3 protein expression attenuates the invasion of clear cell renal cell carcinoma and aggravates anoikis Retraction in /10.3892/mmr.2025.13766". Molecular Medicine Reports 15, no. 3 (2017): 1103-1108. https://doi.org/10.3892/mmr.2017.6136
Copy and paste a formatted citation
x
Spandidos Publications style
Yu M, Lu B, Liu Y, Me Y, Wang L and Li H: Interference with Tim-3 protein expression attenuates the invasion of clear cell renal cell carcinoma and aggravates anoikis Retraction in /10.3892/mmr.2025.13766. Mol Med Rep 15: 1103-1108, 2017.
APA
Yu, M., Lu, B., Liu, Y., Me, Y., Wang, L., & Li, H. (2017). Interference with Tim-3 protein expression attenuates the invasion of clear cell renal cell carcinoma and aggravates anoikis Retraction in /10.3892/mmr.2025.13766. Molecular Medicine Reports, 15, 1103-1108. https://doi.org/10.3892/mmr.2017.6136
MLA
Yu, M., Lu, B., Liu, Y., Me, Y., Wang, L., Li, H."Interference with Tim-3 protein expression attenuates the invasion of clear cell renal cell carcinoma and aggravates anoikis Retraction in /10.3892/mmr.2025.13766". Molecular Medicine Reports 15.3 (2017): 1103-1108.
Chicago
Yu, M., Lu, B., Liu, Y., Me, Y., Wang, L., Li, H."Interference with Tim-3 protein expression attenuates the invasion of clear cell renal cell carcinoma and aggravates anoikis Retraction in /10.3892/mmr.2025.13766". Molecular Medicine Reports 15, no. 3 (2017): 1103-1108. https://doi.org/10.3892/mmr.2017.6136
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