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Dickkopf-related protein 3 negatively regulates the osteogenic differentiation of rat dental follicle cells

  • Authors:
    • Xinchun Zhang
    • Yu Du
    • Junqi Ling
    • Weiqiang Li
    • Yan Liao
    • Xi Wei
  • View Affiliations / Copyright

    Affiliations: Department of Prosthodontics, Guangdong Provincial Key Laboratory of Stomatology, Guanghua School of Stomatology, Sun Yat‑sen University, Guangzhou, Guangdong 510055, P.R. China, Department of Operative Dentistry and Endodontics, Guangdong Provincial Key Laboratory of Stomatology, Guanghua School of Stomatology, Sun Yat‑sen University, Guangzhou, Guangdong 510055, P.R. China, Center for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat‑sen University, Guangzhou, Guangdong 510080, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1673-1681
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    Published online on: February 3, 2017
       https://doi.org/10.3892/mmr.2017.6165
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Abstract

The present study aimed to investigate the effect of Dickkopf-related protein 3 (DKK3) on osteogenic differentiation of rat dental follicle cells (DFCs). A PCR array analysis of Wnt pathway activation in DFCs identified genes dysregulated by mineral induction. Among them, DKK3expression levels were decreased, and further experiments were conducted to investigate its role in DFC osteogenesis. By comparing DFCs grown in normal growth and mineral‑induction media for 4 weeks, the present study confirmed that DKK3 was a potential target gene of osteogenesis through reverse transcription-quantitative polymerase chain reaction (RT‑qPCR) and western blotting (WB). A short hairpin RNA (shRNA) was introduced into DFCs using a lentiviral vector to inhibit DKK3 expression. An alkaline phosphatase (ALP) activity assay and Alizarin Red staining were performed to observe the DKK3‑shRNA DFCs. In addition, the osteogenic differentiation of DKK3‑shRNA DFCs was analyzed by RT‑qPCR and WB. In vivo, DKK3‑shRNA DFCs seeded on hydroxyapatite/β-tricalcium phosphate (HA/TCP) scaffolds were transplanted into the subcutaneous tissue of mice with severe combined immunodeficiency, followed by hematoxylin‑eosin and Masson staining. The results confirmed that DKK3 expression was downregulated during mineral induction in rat DFCs. Lentivirus‑mediated expression of DKK3 shRNA in DFCs promoted calcified‑nodule formation, ALP activity and the expression of β‑catenin, runt‑related transcription factor 2 and osteocalcin, compared with control cells. In vivo, the implanted section presented the majority of newly formed osteoid matrices and collagen, with limited space between the HA/TCP scaffolds and matrices. In conclusion, DKK3 expression negatively regulates the osteogenic differentiation of DFCs and, conversely, downregulation of DKK3 may enhance DFC osteogenesis.
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1 

Ten Cate AR: The development of the periodontium-a largely ectomesenchymally derived unit. Periodontology 2000. 13:9–19. 1997. View Article : Google Scholar : PubMed/NCBI

2 

Wise GE, Lin F and Fan W: Culture and characterization of dental follicle cells from rat molars. Cell Tissue Res. 267:483–492. 1992. View Article : Google Scholar : PubMed/NCBI

3 

Morsczeck C, Götz W, Schierholz J, Zeilhofer F, Kühn U, Möhl C, Sippel C and Hoffmann KH: Isolation of precursor cells (PCs) from human dental follicle of wisdom teeth. Matrix Biol. 24:155–165. 2005. View Article : Google Scholar : PubMed/NCBI

4 

Kemoun P, Laurencin-Dalicieux S, Rue J, Farges JC, Gennero I, Conte-Auriol F, Briand-Mesange F, Gadelorge M, Arzate H, Narayanan AS, et al: Human dental follicle cells acquire cementoblast features under stimulation by BMP-2/−7 and enamel matrix derivatives (EMD) in vitro. Cell Tissue Res. 329:283–294. 2007. View Article : Google Scholar : PubMed/NCBI

5 

Zhao M, Xiao G, Berry JE, Franceschi RT, Reddi A and Somerman MJ: Bone morphogenetic protein 2 induces dental follicle cells to differentiate toward a cementoblast/osteoblast phenotype. J Bone Miner Res. 17:1441–1451. 2002. View Article : Google Scholar : PubMed/NCBI

6 

Viale-Bouroncle S, Bey B, Reichert TE, Schmalz G and Morsczeck C: β-tricalcium-phosphate stimulates the differentiation of dental follicle cells. J Mater Sci Mater Med. 22:1719–1724. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Guo W, Gong K, Shi H, Zhu G, He Y, Ding B, Wen L and Jin Y: Dental follicle cells and treated dentin matrix scaffold for tissue engineering the tooth root. Biomaterials. 33:1291–1302. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Yang B, Chen G, Li J, Zou Q, Xie D, Chen Y, Wang H, Zheng X, Long J, Tang W, et al: Tooth root regeneration using dental follicle cell sheets in combination with a dentin matrix-based scaffold. Biomaterials. 33:2449–2461. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Du Y, Ling J, Wei X, Ning Y, Xie N, Gu H and Yang F: Wnt/β-catenin signaling participates in cementoblast/osteoblast differentiation of dental follicle cells. Connect Tissue Res. 53:390–397. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Khan M, Seppala M, Zoupa M and Cobourne MT: Hedgehog pathway gene expression during early development of the molar tooth root in the mouse. Gene Expr Patterns. 7:239–243. 2007. View Article : Google Scholar : PubMed/NCBI

11 

Viale-Bouroncle S, Gosau M and Morsczeck C: NOTCH1 signaling regulates the BMP2/DLX-3 directed osteogenic differentiation of dental follicle cells. Biochem Biophys Res Commun. 443:500–504. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Xiang L, Chen M, He L, Cai B, Du Y, Zhang X, Zhou C, Wang C, Mao JJ and Ling J: Wnt5a regulates dental follicle stem/progenitor cells of the periodontium. Stem Cell Res Ther. 5:1352014. View Article : Google Scholar : PubMed/NCBI

13 

Jones WM and Bejsovec A: Wingless signaling: An axin to grind. Curr Biol. 13:R479–R481. 2003. View Article : Google Scholar : PubMed/NCBI

14 

McEwen DG and Peifer M: Wnt signaling: Moving in a new direction. Curr Biol. 10:R562–R564. 2000. View Article : Google Scholar : PubMed/NCBI

15 

Kim JA, Choi HK, Kim TM, Leem SH and Oh IH: Regulation of mesenchymal stromal cells through fine tuning of canonical Wnt signaling. Stem Cell Res. 14:356–368. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Liu F and Millar SE: Wnt/beta-catenin signaling in oral tissue development and disease. J Dent Res. 89:318–330. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Scheller EL, Chang J and Wang CY: Wnt/beta-catenin inhibits dental pulp stem cell differentiation. J Dent Res. 87:126–130. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Tian H, Lv P, Ma K, Zhou C and Gao X: Beta-catenin/LEF1 activated enamelin expression in ameloblast-like cells. Biochem Biophys Res Commun. 398:519–524. 2010. View Article : Google Scholar : PubMed/NCBI

19 

Yokoyama N, Golebiewska U, Wang HY and Malbon CC: Wnt-dependent assembly of supermolecular Dishevelled-3-based complexes. J Cell Sci. 123:3693–3702. 2010. View Article : Google Scholar : PubMed/NCBI

20 

Moschovi M, Alexiou GA, Patereli A, Siozos G, Sfakianos G, Prodromou N and Stefanaki K: Immunohistochemical expression of cell-cycle regulators in pediatric embryonal brain tumors. J Neurooncol. 109:529–534. 2012. View Article : Google Scholar : PubMed/NCBI

21 

Krupnik VE, Sharp JD, Jiang C, Robison K, Chickering TW, Amaravadi L, Brown DE, Guyot D, Mays G, Leiby K, et al: Functional and structural diversity of the human Dickkopf gene family. Gene. 238:301–313. 1999. View Article : Google Scholar : PubMed/NCBI

22 

Niehrs C: Function and biological roles of the Dickkopf family of Wnt modulators. Oncogene. 25:7469–7481. 2006. View Article : Google Scholar : PubMed/NCBI

23 

Tsuji T, Miyazaki M, Sakaguchi M, Inoue Y and Namba M: A REIC gene shows down-regulation in human immortalized cells and human tumor-derived cell lines. Biochem Biophys Res Commun. 268:20–24. 2000. View Article : Google Scholar : PubMed/NCBI

24 

Hsieh SY, Hsieh PS, Chiu CT and Chen WY: Dickkopf-3/REIC functions as a suppressor gene of tumor growth. Oncogene. 23:9183–9189. 2004.PubMed/NCBI

25 

Kurose K, Sakaguchi M, Nasu Y, Ebara S, Kaku H, Kariyama R, Arao Y, Miyazaki M, Tsushima T, Namba M, et al: Decreased expression of REIC/Dkk-3 in human renal clear cell carcinoma. J Urol. 171:1314–1318. 2004. View Article : Google Scholar : PubMed/NCBI

26 

Roman-Gomez J, Jimenez-Velasco A, Agirre X, Castillejo JA, Navarro G, Barrios M, Andreu EJ, Prosper F, Heiniger A and Torres A: Transcriptional silencing of the Dickkopfs-3 (Dkk-3) gene by CpGhypermethylation in acute lymphoblastic leukaemia. Br J Cancer. 91:707–713. 2004.PubMed/NCBI

27 

Lodygin D, Epanchintsev A, Menssen A, Diebold J and Hermeking H: Functional epigenomics identifies genes frequently silenced in prostate cancer. Cancer Res. 65:4218–4227. 2005. View Article : Google Scholar : PubMed/NCBI

28 

Kuphal S, Lodermeyer S, Bataille F, Schuierer M, Hoang BH and Bosserhoff AK: Expression of Dickkopf genes is strongly reduced in malignant melanoma. Oncogene. 25:5027–5036. 2006. View Article : Google Scholar : PubMed/NCBI

29 

Fjeld K, Kettunen P, Furmanek T, Kvinnsland IH and Luukko K: Dynamic expression of Wnt signaling-related Dickkopf1, −2 and −3 mRNAs in the developing mouse tooth. Dev Dyn. 233:161–166. 2005. View Article : Google Scholar : PubMed/NCBI

30 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C (T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

31 

Sarkar L and Sharpe PT: Expression of Wnt signalling pathway genes during tooth development. Mech Dev. 85:197–200. 1999. View Article : Google Scholar : PubMed/NCBI

32 

Liu F, Chu EY, Watt B, Zhang Y, Gallant NM, Andl T, Yang SH, Lu MM, Piccolo S, Schmidt-Ullrich R, et al: Wnt/beta-catenin signaling directs multiple stages of tooth morphogenesis. Dev Biol. 313:210–224. 2008. View Article : Google Scholar : PubMed/NCBI

33 

Wei X, Wu L, Ling J, Liu L, Liu S, Liu W, Li M and Xiao Y: Differentially expressed protein profile of human dental pulp cells in the early process of odontoblast-like differentiation in vitro. J Endod. 34:1077–1084. 2008. View Article : Google Scholar : PubMed/NCBI

34 

Wu L, Wei X, Ling J, Liu L, Liu S, Li M and Xiao Y: Early osteogenic differential protein profile detected by proteomic analysis in human periodontal ligament cells. J Periodontal Res. 44:645–656. 2009. View Article : Google Scholar : PubMed/NCBI

35 

Morsczeck C, Petersen J, Völlner F, Driemel O, Reichert T and Beck HC: Proteomic analysis of osteogenic differentiation of dental follicle precursor cells. Electrophoresis. 30:1175–1184. 2009. View Article : Google Scholar : PubMed/NCBI

36 

Morsczeck C, Schmalz G, Reichert TE, Völlner F, Saugspier M, Viale-Bouroncle S and Driemel O: Gene expression profiles of dental follicle cells before and after osteogenic differentiation in vitro. Clin Oral Investig. 13:383–391. 2009. View Article : Google Scholar : PubMed/NCBI

37 

Aslan H, Ravid-Amir O, Clancy BM, Rezvankhah S, Pittman D, Pelled G, Turgeman G, Zilberman Y, Gazit Z, Hoffmann A, et al: Advanced molecular profiling in vivo detects novel function of dickkopf-3 in the regulation of bone formation. J Bone Miner Res. 21:1935–1945. 2006. View Article : Google Scholar : PubMed/NCBI

38 

Onai T, Takai A, Setiamarga DH and Holland LZ: Essential role of Dkk3 for head formation by inhibiting Wnt/β-catenin and Nodal/Vg1 signaling pathways in the basal chordate amphioxus. Evol Dev. 14:338–350. 2012. View Article : Google Scholar : PubMed/NCBI

39 

Monaghan AP, Kioschis P, Wu W, Zuniga A, Bock D, Poustka A, Delius H and Niehrs C: Dickkopf genes are co-ordinately expressed in mesodermal lineages. Mech Dev. 87:45–56. 1999. View Article : Google Scholar : PubMed/NCBI

40 

Silvério KG, Davidson KC, James RG, Adams AM, Foster BL, Nociti FH Jr, Somerman MJ and Moon RT: Wnt/β-catenin pathway regulates bone morphogenetic protein (BMP2)-mediated differentiation of dental follicle cells. J Periodontal Res. 47:309–319. 2012. View Article : Google Scholar : PubMed/NCBI

41 

Jensen SS, Bornstein MM, Dard M, Bosshardt DD and Buser D: Comparative study of biphasic calcium phosphates with different HA/TCP ratios in mandibular bone defects. A long-term histomorphometric study in minipigs. J Biomed Mater Res B Appl Biomater. 90:171–181. 2009.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhang X, Du Y, Ling J, Li W, Liao Y and Wei X: Dickkopf-related protein 3 negatively regulates the osteogenic differentiation of rat dental follicle cells. Mol Med Rep 15: 1673-1681, 2017.
APA
Zhang, X., Du, Y., Ling, J., Li, W., Liao, Y., & Wei, X. (2017). Dickkopf-related protein 3 negatively regulates the osteogenic differentiation of rat dental follicle cells. Molecular Medicine Reports, 15, 1673-1681. https://doi.org/10.3892/mmr.2017.6165
MLA
Zhang, X., Du, Y., Ling, J., Li, W., Liao, Y., Wei, X."Dickkopf-related protein 3 negatively regulates the osteogenic differentiation of rat dental follicle cells". Molecular Medicine Reports 15.4 (2017): 1673-1681.
Chicago
Zhang, X., Du, Y., Ling, J., Li, W., Liao, Y., Wei, X."Dickkopf-related protein 3 negatively regulates the osteogenic differentiation of rat dental follicle cells". Molecular Medicine Reports 15, no. 4 (2017): 1673-1681. https://doi.org/10.3892/mmr.2017.6165
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang X, Du Y, Ling J, Li W, Liao Y and Wei X: Dickkopf-related protein 3 negatively regulates the osteogenic differentiation of rat dental follicle cells. Mol Med Rep 15: 1673-1681, 2017.
APA
Zhang, X., Du, Y., Ling, J., Li, W., Liao, Y., & Wei, X. (2017). Dickkopf-related protein 3 negatively regulates the osteogenic differentiation of rat dental follicle cells. Molecular Medicine Reports, 15, 1673-1681. https://doi.org/10.3892/mmr.2017.6165
MLA
Zhang, X., Du, Y., Ling, J., Li, W., Liao, Y., Wei, X."Dickkopf-related protein 3 negatively regulates the osteogenic differentiation of rat dental follicle cells". Molecular Medicine Reports 15.4 (2017): 1673-1681.
Chicago
Zhang, X., Du, Y., Ling, J., Li, W., Liao, Y., Wei, X."Dickkopf-related protein 3 negatively regulates the osteogenic differentiation of rat dental follicle cells". Molecular Medicine Reports 15, no. 4 (2017): 1673-1681. https://doi.org/10.3892/mmr.2017.6165
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