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Thin magnesium layer confirmed as an antibacterial and biocompatible implant coating in a co‑culture model

  • Authors:
    • Sarah Zaatreh
    • David Haffner
    • Madlen Strauss
    • Thomas Dauben
    • Christiane Zamponi
    • Wolfram Mittelmeier
    • Eckhard Quandt
    • Bernd Kreikemeyer
    • Rainer Bader
  • View Affiliations / Copyright

    Affiliations: Biomechanics and Implant Technology Research Laboratory, Department of Orthopaedics, University Medicine Rostock, D‑18057 Rostock, Germany, Institute for Materials Science, Faculty of Engineering, University of Kiel, D‑24143 Kiel, Germany, Institute of Medical Microbiology, Virology and Hygiene, University Medicine Rostock, D‑18057 Rostock, Germany
    Copyright: © Zaatreh et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1624-1630
    |
    Published online on: February 17, 2017
       https://doi.org/10.3892/mmr.2017.6218
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Abstract

Implant-associated infections commonly result from biofilm‑forming bacteria and present severe complications in total joint arthroplasty. Therefore, there is a requirement for the development of biocompatible implant surfaces that prevent bacterial biofilm formation. The present study coated titanium samples with a thin, rapidly corroding layer of magnesium, which were subsequently investigated with respect to their antibacterial and cytotoxic surface properties using a Staphylococcus epidermidis (S. epidermidis) and human osteoblast (hOB) co‑culture model. Primary hOBs and S. epidermidis were co‑cultured on cylindrical titanium samples (Ti6Al4V) coated with pure magnesium via magnetron sputtering (5 µm thickness) for 7 days. Uncoated titanium test samples served as controls. Vital hOBs were identified by trypan blue staining at days 2 and 7. Planktonic S. epidermidis were quantified by counting the number of colony forming units (CFU). The quantification of biofilm‑bound S. epidermidis on the surfaces of test samples was performed by ultrasonic treatment and CFU counting at days 2 and 7. The number of planktonic and biofilm‑bound S. epidermidis on the magnesium‑coated samples decreased by four orders of magnitude when compared with the titanium control following 7 days of co‑culture. The number of vital hOBs on the magnesium‑coated samples was observed to increase (40,000 cells/ml) when compared with the controls (20,000 cells/ml). The results of the present study indicate that rapidly corroding magnesium‑coated titanium may be a viable coating material that possesses antibacterial and biocompatible properties. A co‑culture test is more rigorous than a monoculture study, as it accounts for confounding effects and assesses additional interactions that are more representative of in vivo situations. These results provide a foundation for the future testing of this type of surface in animals.
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1 

Fallpauschalenbezogene Krankenhausstatistik (DRG-Statistik): Operationen und Prozeduren der vollstationären Patientinnen und Patienten der Krankenhäuser (4-Steller). Statistisches Bundesamt (Destatis), Wiesbaden. 2015.

2 

Darouiche RO: Treatment of infections associated with surgical implants. N Engl J Med. 350:1422–1429. 2004. View Article : Google Scholar : PubMed/NCBI

3 

Walter RP and Blake SM: Re: Two-stage exchange for infected resurfacing arthroplasty use of a novel cement spacer technique. J Arthroplasty. 26:9792011. View Article : Google Scholar : PubMed/NCBI

4 

Harris LG and Richards RG: Staphylococci and implant surfaces: A review. Injury. 37 Suppl 2:S3–S14. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Casey AL, Lambert PA and Elliott TSJ: Staphylococci. Int J Antimicrob Agents. 29 Suppl 3:S23–S32. 2007. View Article : Google Scholar : PubMed/NCBI

6 

Arciola CR, Campoccia D, Speziale P, Montanaro L and Costerton JW: Biofilm formation in Staphylococcus implant infections. A review of molecular mechanisms and implications for biofilm-resistant materials. Biomaterials. 33:5967–5982. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Wilde AH and Ruth JT: Two-stage reimplantation in infected total knee arthroplasty. Clin Orthop. 23–35. 1988.PubMed/NCBI

8 

Vorndran E, Spohn N, Nies B, Rössler S, Storch S and Gbureck U: Mechanical properties and drug release behavior of bioactivated PMMA cements. J Biomater Appl. 26:581–594. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Uchiyama K, Takahira N, Fukushima K, Moriya M, Yamamoto T, Minegishi Y, Sakai R, Itoman M and Takaso M: Two-stage revision total hip arthroplasty for periprosthetic infections using antibiotic-impregnated cement spacers of various types and materials. ScientificWorldJournal. 2013:1472482013. View Article : Google Scholar : PubMed/NCBI

10 

Connaughton A, Childs A, Dylewski S and Sabesan VJ: Biofilm disrupting technology for orthopedic implants: What's on the Horizon? Front Med (Lausanne). 1:222014.PubMed/NCBI

11 

Staiger MP, Pietak AM, Huadmai J and Dias G: Magnesium and its alloys as orthopedic biomaterials: A review. Biomaterials. 27:1728–1734. 2006. View Article : Google Scholar : PubMed/NCBI

12 

Luthringer BJ, Feyerabend F and Willumeit-Römer R: Magnesium-based implants: A mini-review. Magnes Res. 27:142–154. 2014.PubMed/NCBI

13 

Robinson DA, Griffith RW, Shechtman D, Evans RB and Conzemius MG: In vitro antibacterial properties of magnesium metal against Escherichia coliPseudomonas aeruginosa and Staphylococcus aureus. Acta Biomater. 6:1869–1877. 2010. View Article : Google Scholar : PubMed/NCBI

14 

Zaatreh S, Haffner D, Pasold J, Kreikemeyer B, Mittelmeier W, Podbielski A, Quandt E and Bader R: Track A. Biomaterials and biocompatibility 1. Biomed Tech (Berl). 60 Suppl 1:S1–S30. 2015. View Article : Google Scholar : PubMed/NCBI

15 

Cytotoxicity test according to ISO 10993-5. CYTOX. 1999.

16 

Zaatreh S, Wegner K, Strauß M, Pasold J, Mittelmeier W, Podbielski A, Kreikemeyer B and Bader R: Co-culture of S. epidermidis and human osteoblasts on implant surfaces: An advanced in vitro model for implant-associated infections. PloS One. 11:e01515342016. View Article : Google Scholar : PubMed/NCBI

17 

Schlüter K, Zamponi C, Piorra A and Quandt E: Comparison of the corrosion behaviour of bulk and thin film magnesium alloys. Corros Sci. 52:3973–3977. 2010. View Article : Google Scholar

18 

Jonitz A, Lochner K, Lindner T, Hansmann D, Marrot A and Bader R: Oxygen consumption, acidification and migration capacity of human primary osteoblasts within a three-dimensional tantalum scaffold. J Mater Sci Mater Med. 22:2089–2095. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Anleitungen-Marienfeld-Superior. [Internet]. [cited 3 Nov 2016]. http://www.marienfeld-superior.com/index.php/anleitungen.html

20 

Von Eiff C, Peters G and Heilmann C: Pathogenesis of infections due to coagulasenegative staphylococci. Lancet Infect Dis. 2:677–685. 2002. View Article : Google Scholar : PubMed/NCBI

21 

Wang HX, Guan SK, Wang X, Ren CX and Wang LG: In vitro degradation and mechanical integrity of Mg-Zn-Ca alloy coated with Ca-deficient hydroxyapatite by the pulse electrodeposition process. Acta Biomater. 6:1743–1748. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Kirkland NT, Lespagnol J, Birbilis N and Staiger MP: A survey of bio-corrosion rates of magnesium alloys. Corros Sci. 52:287–291. 2010. View Article : Google Scholar

23 

Fischer J, Prosenc MH, Wolff M, Hort N, Willumeit R and Feyerabend F: Interference of magnesium corrosion with tetrazolium-based cytotoxicity assays. Acta Biomater. 6:1813–1823. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Fischer J, Pröfrock D, Hort N, Willumeit R and Feyerabend F: Reprint of: Improved cytotoxicity testing of magnesium materials. Mater Sci Eng B. 176:1773–1777. 2011. View Article : Google Scholar

25 

Schlüter K, Zamponi C, Hapke J, Hort N, Kainer KU and Quandt E: Mechanical properties and corrosion behaviour of freestanding, precipitate-free magnesium WE43 thin films. Int J Mater Res. 104:286–292. 2013. View Article : Google Scholar

26 

Schlüter K, Shi Z, Zamponi C, Cao F, Quandt E and Atrens A: Corrosion performance and mechanical properties of sputter-deposited MgY and MgGd alloys. Corros Sci. 78:43–54. 2014. View Article : Google Scholar

27 

Sanchez AH Martinez, Luthringer BJC, Feyerabend F and Willumeit R: Mg and Mg alloys: How comparable are in vitro and in vivo corrosion rates? A review. Acta Biomater. 13:16–31. 2015. View Article : Google Scholar : PubMed/NCBI

28 

Willumeit R, Feyerabend F and Huber N: Magnesium degradation as determined by artificial neural networks. Acta Biomater. 9:8722–8729. 2013. View Article : Google Scholar : PubMed/NCBI

29 

Charyeva O, Neilands J, Svensäter G and Wennerberg A: Bacterial biofilm formation on resorbing magnesium implants. Open J Med Microbiol. 5:1–11. 2015. View Article : Google Scholar

30 

Gasol JM and Del Giorgio PA: Using flow cytometry for counting natural planktonic bacteria and understanding the structure of planktonic bacterial communities. Sci Mar. 64:197–224. 2000. View Article : Google Scholar

31 

Lee JH, Wang H, Kaplan JB and Lee WY: Microfluidic approach to create three-dimensional tissue models for biofilm-related infection of orthopaedic implants. Tissue Eng Part C Methods. 17:39–48. 2011. View Article : Google Scholar : PubMed/NCBI

32 

Kaji H, Camci-Unal G, Langer R and Khademhosseini A: Engineering systems for the generation of patterned co-cultures for controlling cell-cell interactions. Biochim Biophys Acta. 1810:239–250. 2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zaatreh S, Haffner D, Strauss M, Dauben T, Zamponi C, Mittelmeier W, Quandt E, Kreikemeyer B and Bader R: Thin magnesium layer confirmed as an antibacterial and biocompatible implant coating in a co‑culture model. Mol Med Rep 15: 1624-1630, 2017.
APA
Zaatreh, S., Haffner, D., Strauss, M., Dauben, T., Zamponi, C., Mittelmeier, W. ... Bader, R. (2017). Thin magnesium layer confirmed as an antibacterial and biocompatible implant coating in a co‑culture model. Molecular Medicine Reports, 15, 1624-1630. https://doi.org/10.3892/mmr.2017.6218
MLA
Zaatreh, S., Haffner, D., Strauss, M., Dauben, T., Zamponi, C., Mittelmeier, W., Quandt, E., Kreikemeyer, B., Bader, R."Thin magnesium layer confirmed as an antibacterial and biocompatible implant coating in a co‑culture model". Molecular Medicine Reports 15.4 (2017): 1624-1630.
Chicago
Zaatreh, S., Haffner, D., Strauss, M., Dauben, T., Zamponi, C., Mittelmeier, W., Quandt, E., Kreikemeyer, B., Bader, R."Thin magnesium layer confirmed as an antibacterial and biocompatible implant coating in a co‑culture model". Molecular Medicine Reports 15, no. 4 (2017): 1624-1630. https://doi.org/10.3892/mmr.2017.6218
Copy and paste a formatted citation
x
Spandidos Publications style
Zaatreh S, Haffner D, Strauss M, Dauben T, Zamponi C, Mittelmeier W, Quandt E, Kreikemeyer B and Bader R: Thin magnesium layer confirmed as an antibacterial and biocompatible implant coating in a co‑culture model. Mol Med Rep 15: 1624-1630, 2017.
APA
Zaatreh, S., Haffner, D., Strauss, M., Dauben, T., Zamponi, C., Mittelmeier, W. ... Bader, R. (2017). Thin magnesium layer confirmed as an antibacterial and biocompatible implant coating in a co‑culture model. Molecular Medicine Reports, 15, 1624-1630. https://doi.org/10.3892/mmr.2017.6218
MLA
Zaatreh, S., Haffner, D., Strauss, M., Dauben, T., Zamponi, C., Mittelmeier, W., Quandt, E., Kreikemeyer, B., Bader, R."Thin magnesium layer confirmed as an antibacterial and biocompatible implant coating in a co‑culture model". Molecular Medicine Reports 15.4 (2017): 1624-1630.
Chicago
Zaatreh, S., Haffner, D., Strauss, M., Dauben, T., Zamponi, C., Mittelmeier, W., Quandt, E., Kreikemeyer, B., Bader, R."Thin magnesium layer confirmed as an antibacterial and biocompatible implant coating in a co‑culture model". Molecular Medicine Reports 15, no. 4 (2017): 1624-1630. https://doi.org/10.3892/mmr.2017.6218
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