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Article

Plumbagin prevents osteoarthritis in human chondrocytes through Nrf-2 activation

  • Authors:
    • Yong‑Xian Guo
    • Liang Liu
    • Dong‑Zhan Yan
    • Jian‑Ping Guo
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, The Second People's Hospital of Liaocheng, Affiliated Hospital of Taishan Medical University, Linqing, Shandong 252600, P.R. China, Department II of Orthopedics, Linzi District People's Hospital, Zibo, Shandong 255400, P.R. China, Department of Orthopedics, Linqing City People's Hospital, Linqing, Shandong 252600, P.R. China, Department of Orthopedics, The Third People's Hospital of Chongqing, Chongqing 400014, P.R. China
  • Pages: 2333-2338
    |
    Published online on: February 22, 2017
       https://doi.org/10.3892/mmr.2017.6234
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Abstract

Osteoarthritis (OA) is an inflammatory disorder dealing with the focal degradation of articular cartilage. Oxidative stress and inflammation are the major events in OA. The present study aimed at identifying the mechanism of the potent antioxidant, plumbagin, in protecting against hydrogen peroxide (H2O2)‑induced chondrocyte oxidative stress and inflammatory signaling. Oxidative stress was determined by measuring reactive oxygen species, lipid peroxidation, non‑enzymic (glutathione; GSH) and enzymic antioxidant activities (GSH, glutathione S‑transferase, glutathione peroxidase, superoxide dismutase, catalase). Expression levels of nuclear factor (erythroid‑derived 2)‑like 2 (Nrf‑2), heme oxygenase 1 (HO‑1), NAD(P)H:quinone oxidoreductase 1 (NQO‑1), nuclear factor‑κB (NF‑κB), cyclooxygenase‑2 (COX‑2) and inducible NO synthase (iNOS) were determined by western blot analysis. Pro‑inflammatory cytokine expression levels were assessed using ELISA. Results from reactive oxygen species generation, lipid peroxidation content and antioxidant enzyme activities demonstrated that plumbagin significantly inhibited oxidative stress status in H2O2‑induced chondrocytes. In addition, plumbagin modulated transcription factors involved in redox and inflammation regulation, including NF‑κB and Nrf‑2, by nuclear expression. plumbagin enhanced antioxidant status by increasing the expression levels of Nrf‑2 target genes, including HO‑1 and NQO‑1. An anti‑inflammatory effect against chondrocyte‑induced inflammation was demonstrated by downregulating COX‑2, iNOS and pro‑inflammatory cytokine expression levels (tumor necrosis factor‑α, interleukin (IL)‑6 and IL‑8). The present study identified strong evidence for a protective role of plumbagin against H2O2-induced oxidative stress and inflammation in chondrocytes by modulating redox signaling transcription factors.
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Copy and paste a formatted citation
Spandidos Publications style
Guo YX, Liu L, Yan DZ and Guo JP: Plumbagin prevents osteoarthritis in human chondrocytes through Nrf-2 activation. Mol Med Rep 15: 2333-2338, 2017.
APA
Guo, Y., Liu, L., Yan, D., & Guo, J. (2017). Plumbagin prevents osteoarthritis in human chondrocytes through Nrf-2 activation. Molecular Medicine Reports, 15, 2333-2338. https://doi.org/10.3892/mmr.2017.6234
MLA
Guo, Y., Liu, L., Yan, D., Guo, J."Plumbagin prevents osteoarthritis in human chondrocytes through Nrf-2 activation". Molecular Medicine Reports 15.4 (2017): 2333-2338.
Chicago
Guo, Y., Liu, L., Yan, D., Guo, J."Plumbagin prevents osteoarthritis in human chondrocytes through Nrf-2 activation". Molecular Medicine Reports 15, no. 4 (2017): 2333-2338. https://doi.org/10.3892/mmr.2017.6234
Copy and paste a formatted citation
x
Spandidos Publications style
Guo YX, Liu L, Yan DZ and Guo JP: Plumbagin prevents osteoarthritis in human chondrocytes through Nrf-2 activation. Mol Med Rep 15: 2333-2338, 2017.
APA
Guo, Y., Liu, L., Yan, D., & Guo, J. (2017). Plumbagin prevents osteoarthritis in human chondrocytes through Nrf-2 activation. Molecular Medicine Reports, 15, 2333-2338. https://doi.org/10.3892/mmr.2017.6234
MLA
Guo, Y., Liu, L., Yan, D., Guo, J."Plumbagin prevents osteoarthritis in human chondrocytes through Nrf-2 activation". Molecular Medicine Reports 15.4 (2017): 2333-2338.
Chicago
Guo, Y., Liu, L., Yan, D., Guo, J."Plumbagin prevents osteoarthritis in human chondrocytes through Nrf-2 activation". Molecular Medicine Reports 15, no. 4 (2017): 2333-2338. https://doi.org/10.3892/mmr.2017.6234
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