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Article

Identification of the long non‑coding RNA LET as a novel tumor suppressor in gastric cancer

  • Authors:
    • Jingjing Tian
    • Xibao Hu
    • Wei Gao
    • Jie Zhang
    • Ming Chen
    • Xinrong Zhang
    • Junhong Ma
    • Hongxia Yuan
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, NanKai Hospital, Tianjin 300100, P.R. China, First Teaching Hospital, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, P.R. China, School of Management, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, P.R. China
  • Pages: 2229-2234
    |
    Published online on: February 28, 2017
       https://doi.org/10.3892/mmr.2017.6263
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Abstract

Long non-coding RNAs (lncRNAs) have emerged recently as important factors in regulating fundamental biological processes. Alterations in the expression and function of lncRNAs have been observed to promote tumor formation, progression and metastasis. Although downregulation of the expression levels of LET lncRNA in several tumors has been reported, its role in gastric cancer remains unknown. The aim of the present study was to investigate the expression and function of LET in gastric cancer development. The expression levels of LET in 37 pairs of gastric cancer and adjacent non‑tumor tissues were detected by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). In addition, LET expression in gastric cancer cell lines was analyzed by RT‑qPCR assay analysis. Furthermore, the impact of LET on cell proliferation, migration and apoptosis were detected using the cell counting kit‑8, wound scratch and ELISA assays, respectively. The results demonstrated that the expression level of LET was downregulated in gastric cancer tissues and cell lines (SGC‑7901 and MGC‑803) compared with normal tissues and a normal human gastric epithelial cell line (GES‑1). Restoration of LET expression using a synthesized recombinant overexpression vector transfected into SGC‑7901 and MGC‑803 cells, significantly inhibited cell proliferation and migration, and promoted cell apoptosis in vitro. The present study is the first to demonstrate that LET may function as a tumor suppressor in gastric cancer. The results indicate that LET may be a promising biomarker and/or a therapeutic target for gastric cancer.
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1 

Siegel R, Naishadham D and Jemal A: Cancer statistics, 2013. CA Cancer J Clin. 63:11–30. 2013. View Article : Google Scholar : PubMed/NCBI

2 

Hu N, Wang Z, Song X, Wei L, Kim BS, Freedman ND, Baek J, Burdette L, Chang J, Chung C, et al: Genome-wide association study of gastric adenocarcinoma in Asia: A comparison of associations between cardia and non-cardia tumours. Gut. 65:1611–1688. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Nakajima T: Gastric cancer treatment guidelines in Japan. Gastric Cancer. 5:1–5. 2002. View Article : Google Scholar : PubMed/NCBI

4 

Vogiatzi P, Vindigni C, Roviello F, Renieri A and Giordano A: Deciphering the underlying genetic and epigenetic events leading to gastric carcinogenesis. J Cell Physiol. 211:287–295. 2007. View Article : Google Scholar : PubMed/NCBI

5 

Crew KD and Neugut AI: Epidemiology of gastric cancer. World J Gastroenterol. 12:354–362. 2006. View Article : Google Scholar : PubMed/NCBI

6 

Costa FF: Non-coding RNAs: Meet thy masters. Bioessays. 32:599–608. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Liu J, Shen L, Yao J, Li Y, Wang Y, Chen H and Geng P: Forkhead box C1 promoter upstream transcript, a novel long non-coding RNA, regulates proliferation and migration in basal-like breast cancer. Mol Med Rep. 11:3155–3159. 2015.PubMed/NCBI

8 

Tian F, Tang Z, Song G, Pan Y, He B, Bao Q and Wang S: Loss of imprinting of IGF2 correlates with hypomethylation of the H19 differentially methylated region in the tumor tissue of colorectal cancer patients. Mol Med Rep. 5:1536–1540. 2012.PubMed/NCBI

9 

Eades G, Wolfson B, Zhang Y, Li Q, Yao Y and Zhou Q: lincRNA-RoR and miR-145 regulate invasion in triple-negative breast cancer via targeting ARF6. Mol Cancer Res. 13:330–338. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Rusek AM, Abba M, Eljaszewicz A, Moniuszko M, Niklinski J and Allgayer H: MicroRNA modulators of epigenetic regulation, the tumor microenvironment and the immune system in lung cancer. Mol Cancer. 14:342015. View Article : Google Scholar : PubMed/NCBI

11 

Cheng CJ, Bahal R, Babar IA, Pincus Z, Barrera F, Liu C, Svoronos A, Braddock DT, Glazer PM, Engelman DM, et al: MicroRNA silencing for cancer therapy targeted to the tumour microenvironment. Nature. 518:107–110. 2015. View Article : Google Scholar : PubMed/NCBI

12 

Kong R, Zhang EB, Yin DD, You LH, Xu TP, Chen WM, Xia R, Wan L, Sun M, Wang ZX, et al: Long noncoding RNA PVT1 indicates a poor prognosis of gastric cancer and promotes cell proliferation through epigenetically regulating p15 and p16. Mol Cancer. 14:822015. View Article : Google Scholar : PubMed/NCBI

13 

Pang Q, Ge J, Shao Y, Sun W, Song H, Xia T, Xiao B and Guo J: Increased expression of long intergenic non-coding RNA LINC00152 in gastric cancer and its clinical significance. Tumour Biol. 35:5441–5447. 2014. View Article : Google Scholar : PubMed/NCBI

14 

Wang CY, Hua L, Yao KH, Chen JT, Zhang JJ and Hu JH: Long non-coding RNA CCAT2 is up-regulated in gastric cancer and associated with poor prognosis. Int J Clin Exp Pathol. 8:779–785. 2015.PubMed/NCBI

15 

Gan L, Xu M, Zhang Y, Zhang X and Guo W: Focusing on long noncoding RNA dysregulation in gastric cancer. Tumour Biol. 36:129–141. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Zhou B, Jing XY, Wu JQ, Xi HF and Lu GJ: Down-regulation of long non-coding RNA LET is associated with poor prognosis in gastric cancer. Int J Clin Exp Pathol. 7:8893–8898. 2014.PubMed/NCBI

17 

Strong VE, D'Amico TA, Kleinberg L and Ajani J: Impact of the 7th Edition AJCC staging classification on the NCCN clinical practice guidelines in oncology for gastric and esophageal cancers. J Natl Compr Canc Netw. 11:60–66. 2013.PubMed/NCBI

18 

Schmittgen TD and Livak KJ: Analyzing real-time PCR data by the comparative C(T) method. Nat Protoc. 3:1101–1108. 2008. View Article : Google Scholar : PubMed/NCBI

19 

Li J, Tian H, Yang J and Gong Z: Long Noncoding RNAs regulate cell growth, proliferation, and apoptosis. DNA Cell Biol. 35:459–470. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Yamamoto H, Watanabe Y, Maehata T, Morita R, Yoshida Y, Oikawa R, Ishigooka S, Ozawa S, Matsuo Y, Hosoya K, et al: An updated review of gastric cancer in the next-generation sequencing era: Insights from bench to bedside and vice versa. World J Gastroenterol. 20:3927–3937. 2014. View Article : Google Scholar : PubMed/NCBI

21 

Smith MG, Hold GL, Tahara E and El-Omar EM: Cellular and molecular aspects of gastric cancer. World J Gastroenterol. 12:2979–2990. 2006. View Article : Google Scholar : PubMed/NCBI

22 

Szczesniak MW and Makalowska I: lncRNA-RNA interactions across the human transcriptome. PLoS One. 11:e01503532016. View Article : Google Scholar : PubMed/NCBI

23 

Liu B, Sun L and Song E: Pinched by RNA ‘fingers’: Long noncoding RNAs hitting signal transduction pathways. Mol Cell Oncol. 3:e10465822016. View Article : Google Scholar : PubMed/NCBI

24 

Li H, An J, Wu M, Zheng Q, Gui X, Li T, Pu H and Lu D: LncRNA HOTAIR promotes human liver cancer stem cell malignant growth through downregulation of SETD2. Oncotarget. 6:27847–27864. 2015. View Article : Google Scholar : PubMed/NCBI

25 

Salameh A, Lee AK, Cardó-Vila M, Nunes DN, Efstathiou E, Staquicini FI, Dobroff AS, Marchiò S, Navone NM, Hosoya H, et al: PRUNE2 is a human prostate cancer suppressor regulated by the intronic long noncoding RNA PCA3. Proc Natl Acad Sci USA. 112:8403–8408. 2015. View Article : Google Scholar : PubMed/NCBI

26 

Zhang H, Zhao L, Wang YX, Xi M, Liu SL and Luo LL: Long non-coding RNA HOTTIP is correlated with progression and prognosis in tongue squamous cell carcinoma. Tumour Biol. 36:8805–8809. 2015. View Article : Google Scholar : PubMed/NCBI

27 

Liu L, Liu Y, Zhuang C, Xu W, Fu X, Lv Z, Wu H, Mou L, Zhao G, Cai Z and Huang W: Inducing cell growth arrest and apoptosis by silencing long non-coding RNA PCAT-1 in human bladder cancer. Tumour Biol. 36:7685–7689. 2015. View Article : Google Scholar : PubMed/NCBI

28 

Jiang S, Wang HL and Yang J: Low expression of long non-coding RNA LET inhibits carcinogenesis of cervical cancer. Int J Clin Exp Pathol. 8:806–811. 2015.PubMed/NCBI

29 

Ma MZ, Kong X, Weng MZ, Zhang MD, Qin YY, Gong W, Zhang WJ and Quan ZW: Long non-coding RNA-LET is a positive prognostic factor and exhibits tumor-suppressive activity in gallbladder cancer. Mol Carcinog. 54:1397–1406. 2015. View Article : Google Scholar : PubMed/NCBI

30 

Yang F, Huo XS, Yuan SX, Zhang L, Zhou WP, Wang F and Sun SH: Repression of the long noncoding RNA-LET by histone deacetylase 3 contributes to hypoxia-mediated metastasis. Mol Cell. 49:1083–1096. 2013. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Tian J, Hu X, Gao W, Zhang J, Chen M, Zhang X, Ma J and Yuan H: Identification of the long non‑coding RNA LET as a novel tumor suppressor in gastric cancer. Mol Med Rep 15: 2229-2234, 2017.
APA
Tian, J., Hu, X., Gao, W., Zhang, J., Chen, M., Zhang, X. ... Yuan, H. (2017). Identification of the long non‑coding RNA LET as a novel tumor suppressor in gastric cancer. Molecular Medicine Reports, 15, 2229-2234. https://doi.org/10.3892/mmr.2017.6263
MLA
Tian, J., Hu, X., Gao, W., Zhang, J., Chen, M., Zhang, X., Ma, J., Yuan, H."Identification of the long non‑coding RNA LET as a novel tumor suppressor in gastric cancer". Molecular Medicine Reports 15.4 (2017): 2229-2234.
Chicago
Tian, J., Hu, X., Gao, W., Zhang, J., Chen, M., Zhang, X., Ma, J., Yuan, H."Identification of the long non‑coding RNA LET as a novel tumor suppressor in gastric cancer". Molecular Medicine Reports 15, no. 4 (2017): 2229-2234. https://doi.org/10.3892/mmr.2017.6263
Copy and paste a formatted citation
x
Spandidos Publications style
Tian J, Hu X, Gao W, Zhang J, Chen M, Zhang X, Ma J and Yuan H: Identification of the long non‑coding RNA LET as a novel tumor suppressor in gastric cancer. Mol Med Rep 15: 2229-2234, 2017.
APA
Tian, J., Hu, X., Gao, W., Zhang, J., Chen, M., Zhang, X. ... Yuan, H. (2017). Identification of the long non‑coding RNA LET as a novel tumor suppressor in gastric cancer. Molecular Medicine Reports, 15, 2229-2234. https://doi.org/10.3892/mmr.2017.6263
MLA
Tian, J., Hu, X., Gao, W., Zhang, J., Chen, M., Zhang, X., Ma, J., Yuan, H."Identification of the long non‑coding RNA LET as a novel tumor suppressor in gastric cancer". Molecular Medicine Reports 15.4 (2017): 2229-2234.
Chicago
Tian, J., Hu, X., Gao, W., Zhang, J., Chen, M., Zhang, X., Ma, J., Yuan, H."Identification of the long non‑coding RNA LET as a novel tumor suppressor in gastric cancer". Molecular Medicine Reports 15, no. 4 (2017): 2229-2234. https://doi.org/10.3892/mmr.2017.6263
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