1,25(OH)2D3/VDR attenuates high glucose‑induced epithelial‑mesenchymal transition in human peritoneal mesothelial cells via the TGFβ/Smad3 pathway

  • Authors:
    • Lina Yang
    • Lan Wu
    • Xiuli Zhang
    • Ye Hu
    • Yi Fan
    • Jianfei Ma
  • View Affiliations

  • Published online on: March 1, 2017     https://doi.org/10.3892/mmr.2017.6276
  • Pages: 2273-2279
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Abstract

Epithelial-mesenchymal transition (EMT) has been recognized to accelerate peritoneal membrane dysfunction. 1,25(OH)2D3/vitamin D receptor (VDR) is important for preventing various types of EMT in vivo. However, its function on EMT and inflammation of human peritoneal mesothelial cells (HPMCs) remains to be elucidated. Therefore, the present study investigated the effects of 1,25(OH)2D3/VDR on high glucose (HG)‑induced EMT and inflammation in HPMCs and the underlying molecular mechanism. It was determined that HG reduced VDR expression, increased inflammatory cytokine expression, including transforming growth factor β (TGFβ) and interleukin‑6 (IL‑6) and phosphorylated‑SMAD family member 3 (p‑Smad3) expression. EMT was promoted as the expression level of the epithelial marker E‑cadherin was reduced, whereas expression levels of the mesenchymal markers α‑SMA and FN were increased. 1,25(OH)2D3 pretreatment inhibited the expression of inflammatory cytokines in HPMCs and attenuated HG‑induced EMT, possibly through inhibition of the TGFβ/Smad pathway by binding to its receptor VDR.
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April-2017
Volume 15 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Yang L, Wu L, Zhang X, Hu Y, Fan Y and Ma J: 1,25(OH)2D3/VDR attenuates high glucose‑induced epithelial‑mesenchymal transition in human peritoneal mesothelial cells via the TGFβ/Smad3 pathway. Mol Med Rep 15: 2273-2279, 2017
APA
Yang, L., Wu, L., Zhang, X., Hu, Y., Fan, Y., & Ma, J. (2017). 1,25(OH)2D3/VDR attenuates high glucose‑induced epithelial‑mesenchymal transition in human peritoneal mesothelial cells via the TGFβ/Smad3 pathway. Molecular Medicine Reports, 15, 2273-2279. https://doi.org/10.3892/mmr.2017.6276
MLA
Yang, L., Wu, L., Zhang, X., Hu, Y., Fan, Y., Ma, J."1,25(OH)2D3/VDR attenuates high glucose‑induced epithelial‑mesenchymal transition in human peritoneal mesothelial cells via the TGFβ/Smad3 pathway". Molecular Medicine Reports 15.4 (2017): 2273-2279.
Chicago
Yang, L., Wu, L., Zhang, X., Hu, Y., Fan, Y., Ma, J."1,25(OH)2D3/VDR attenuates high glucose‑induced epithelial‑mesenchymal transition in human peritoneal mesothelial cells via the TGFβ/Smad3 pathway". Molecular Medicine Reports 15, no. 4 (2017): 2273-2279. https://doi.org/10.3892/mmr.2017.6276