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Article

1,25(OH)2D3/VDR attenuates high glucose‑induced epithelial‑mesenchymal transition in human peritoneal mesothelial cells via the TGFβ/Smad3 pathway

  • Authors:
    • Lina Yang
    • Lan Wu
    • Xiuli Zhang
    • Ye Hu
    • Yi Fan
    • Jianfei Ma
  • View Affiliations / Copyright

    Affiliations: Department of Nephrology, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China, Department of Geriatrics, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China, Department of Nephrology, Benxi Center Hospital, China Medical University, Benxi, Liaoning 117000, P.R. China
  • Pages: 2273-2279
    |
    Published online on: March 1, 2017
       https://doi.org/10.3892/mmr.2017.6276
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Abstract

Epithelial-mesenchymal transition (EMT) has been recognized to accelerate peritoneal membrane dysfunction. 1,25(OH)2D3/vitamin D receptor (VDR) is important for preventing various types of EMT in vivo. However, its function on EMT and inflammation of human peritoneal mesothelial cells (HPMCs) remains to be elucidated. Therefore, the present study investigated the effects of 1,25(OH)2D3/VDR on high glucose (HG)‑induced EMT and inflammation in HPMCs and the underlying molecular mechanism. It was determined that HG reduced VDR expression, increased inflammatory cytokine expression, including transforming growth factor β (TGFβ) and interleukin‑6 (IL‑6) and phosphorylated‑SMAD family member 3 (p‑Smad3) expression. EMT was promoted as the expression level of the epithelial marker E‑cadherin was reduced, whereas expression levels of the mesenchymal markers α‑SMA and FN were increased. 1,25(OH)2D3 pretreatment inhibited the expression of inflammatory cytokines in HPMCs and attenuated HG‑induced EMT, possibly through inhibition of the TGFβ/Smad pathway by binding to its receptor VDR.
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1 

Williams JD, Craig KJ, Topley N, Von Ruhland C, Fallon M, Newman GR, Mackenzie RK and Williams GT: Peritoneal Biopsy Study Group: Morphologic changes in the peritoneal membrane of patients with renal disease. J Am Soc Nephrol. 13:470–479. 2002.PubMed/NCBI

2 

Ding X, Ma M, Teng J, Shao F, Teng RK, Zhou S, Zhang Y, Wu E and Wang X: Numb induces E-cadherin adhesion dissolution, cytoskeleton reorganization, and migration in tubular epithelial cells contributing to renal fibrosis. Curr Mol Med. 15:368–379. 2015. View Article : Google Scholar : PubMed/NCBI

3 

Zhao S, Zhang Y, Zheng X, Tu X, Li H, Chen J, Zang Y and Zhang J: Loss of microRNA-101 promotes epithelial to mesenchymal transition in hepatocytes. J Cell Physiol. 230:2706–2717. 2015. View Article : Google Scholar : PubMed/NCBI

4 

Strippoli R, Loureiro J, Moreno V, Benedicto I, Lozano ML, Barreiro O, Pellinen T, Minguet S, Foronda M, Osteso MT, et al: Caveolin-1 deficiency induces a MEK-ERK1/2-Snail-1-dependent epithelial-mesenchymal transition and fibrosis during peritoneal dialysis. EMBO Mol Med. 7:3572015. View Article : Google Scholar : PubMed/NCBI

5 

He L, Lou W, Ji L, Liang W, Zhou M, Xu G, Zhao L, Huang C, Li R, Wang H, et al: Serum response factor accelerates the high glucose-induced Epithelial-to-Mesenchymal Transition (EMT) via snail signaling in human peritoneal mesothelial cells. PLoS One. 9:e1085932014. View Article : Google Scholar : PubMed/NCBI

6 

Thiery JP and Sleeman JP: Complex networks orchestrate epithelial-mesenchymal transitions. Nat Rev Mol Cell Biol. 7:131–142. 2006. View Article : Google Scholar : PubMed/NCBI

7 

Liu J, Zeng L, Zhao Y, Zhu B, Ren W and Wu C: Selenium suppresses lipopolysaccharide- induced fibrosis in peritoneal mesothelial cells through inhibition of epithelial-to-mesenchymal transition. Biol Trace Elem Res. 161:202–209. 2014. View Article : Google Scholar : PubMed/NCBI

8 

Yu MA, Shin KS, Kim JH, Kim YI, Chung SS, Park SH, Kim YL and Kang DH: Hgf and bmp-7 ameliorate high glucose-induced epithelial-to-mesenchymal transition of peritoneal mesothelium. J Am Soc Nephrol. 20:567–581. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Zhang X, Wang J, Fan Y, Yang L, Wang L and Ma J: Zinc supplementation attenuates high glucose-induced epithelial-to-mesenchymal transition of peritoneal mesothelial cells. Biol Trace Elem Res. 150:229–235. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Kanasaki K, Taduri G and Koya D: Diabetic nephropathy: The role of inflammation in fibroblast activation and kidney fibrosis. Front Endocrinol (Lausanne). 4:72013.PubMed/NCBI

11 

Gocek E, Kiełbiński M, Wyłób P, Kutner A and Marcinkowska E: Side-chain modified vitamin D analogs induce rapid accumulation of VDR in the cell nuclei proportionately to their differentiation-inducing potential. Steroids. 73:1359–1366. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Kim CS, Joo SY, Lee KE, Choi JS, Bae EH, Ma SK, Kim SH, Lee J and Kim SW: Paricalcitol attenuates 4-hydroxy-2-hexenal-induced inflammation and epithelial-mesenchymal transition in human renal proximal tubular epithelial cells. PLoS One. 8:e631862013. View Article : Google Scholar : PubMed/NCBI

13 

Fischer KD and Agrawal DK: Vitamin D regulating TGF-β induced epithelial-mesenchymal transition. Respir Res. 15:1462014. View Article : Google Scholar : PubMed/NCBI

14 

Yang L, Wang J, Fan Y, Chen S, Wang L and Ma J: Effect of 1,25(OH)(2)D(3) on rat peritoneal mesothelial cells treated with high glucose plus lipopolysaccharide. Cell Immunol. 271:173–179. 2011. View Article : Google Scholar : PubMed/NCBI

15 

Yao Q, Pawlaczyk K, Ayala ER, Styszynski A, Breborowicz A, Heimburger O, Qian JQ, Stenvinkel P, Lindholm B and Axelsson J: The role of the TGF/Smad signaling pathway in peritoneal fibrosis induced by peritoneal dialysis solutions. Nephron Exp Nephrol. 109:e71–e78. 2008. View Article : Google Scholar : PubMed/NCBI

16 

Zhu L, Kong M, Han YP, Bai L, Zhang X, Chen Y, Zheng S, Yuan H and Duan Z: Spontaneous liver fibrosis induced by long term dietary vitamin D deficiency in adult mice is related to chronic inflammation and enhanced apoptosis. Can J Physiol Pharmacol. 93:385–394. 2015. View Article : Google Scholar : PubMed/NCBI

17 

Greń A: Effects of vitamin E, C and D supplementation on inflammation and oxidative stress in streptozotocin-induced diabetic mice. Int J Vitam Nutr Res. 83:168–175. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Duggan C, de Dieu Tapsoba J, Mason C, Imayama I, Korde L, Wang CY and McTiernan A: Effect of Vitamin D3 supplementation in combination with weight loss on inflammatory biomarkers in postmenopausal women: a randomized controlled trial. Cancer Prev Res (Phila). 8:628–635. 2015. View Article : Google Scholar : PubMed/NCBI

19 

Zhou Q, Yang M, Lan H and Yu X: miR-30a negatively regulates TGF-β1-induced epithelial-mesenchymal transition and peritoneal fibrosis by targeting Snai1. Am J Pathol. 183:808–819. 2013. View Article : Google Scholar : PubMed/NCBI

20 

Xiong M, Gong J, Liu Y, Xiang R and Tan X: Loss of vitamin D receptor in chronic kidney disease: A potential mechanism linking inflammation to epithelial-to-mesenchymal transition. Am J Physiol Renal Physiol. 303:F1107–F1115. 2012. View Article : Google Scholar : PubMed/NCBI

21 

Upadhyay SK, Verone A, Shoemaker S, Qin M, Liu S, Campbell M and Hershberger PA: 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) signaling capacity and the epithelial-mesenchymal transition in non-small cell lung cancer (NSCLC): Implications for Use of 1,25(OH)2D3 in NSCLC treatment. Cancers (Basel). 5:1504–1521. 2013. View Article : Google Scholar : PubMed/NCBI

22 

Li Z, Guo J, Xie K and Zheng S: Vitamin D receptor signaling and pancreatic cancer cell EMT. Curr Pharm Des. 21:1262–1267. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Aroeira LS, Aguilera A, Sánchez-Tomero JA, Bajo MA, del Peso G, Jiménez-Heffernan JA, Selgas R and López-Cabrera M: Epithelial to mesenchymal transition and peritoneal membrane failure in peritoneal dialysis patients: Pathologic significance and potential therapeutic interventions. J Am Soc Nephrol. 18:2004–2013. 2007. View Article : Google Scholar : PubMed/NCBI

24 

Del Peso G, Jiménez-Heffernan JA, Bajo MA, Aroeira LS, Aguilera A, Fernández-Perpén A, Cirugeda A, Castro MJ, De Gracia R, Sánchez-Villanueva R, et al: Epithelial-to-mesenchymal transition of mesothelial cells is an early event during peritoneal dialysis and is associated with high peritoneal transport. Kidney Int Suppl. S26–S33. 2008. View Article : Google Scholar : PubMed/NCBI

25 

Yokoi H, Kasahara M, Mori K, Ogawa Y, Kuwabara T, Imamaki H, Kawanishi T, Koga K, Ishii A, Kato Y, et al: Pleiotrophin triggers inflammation and increased peritoneal permeability leading to peritoneal fibrosis. Kidney Int. 81:160–169. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Gangji AS, Brimble KS and Margetts PJ: Association between markers of inflammation, fibrosis and hypervolemia in peritoneal dialysis patients. Blood Purif. 28:354–358. 2009. View Article : Google Scholar : PubMed/NCBI

27 

Lee TW, Kao YH, Lee TI, Chang CJ, Lien GS and Chen YJ: Calcitriol modulates receptor for advanced glycation end products (RAGE) in diabetic hearts. Int J Cardiol. 173:236–241. 2014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yang L, Wu L, Zhang X, Hu Y, Fan Y and Ma J: 1,25(OH)2D3/VDR attenuates high glucose‑induced epithelial‑mesenchymal transition in human peritoneal mesothelial cells via the TGFβ/Smad3 pathway. Mol Med Rep 15: 2273-2279, 2017.
APA
Yang, L., Wu, L., Zhang, X., Hu, Y., Fan, Y., & Ma, J. (2017). 1,25(OH)2D3/VDR attenuates high glucose‑induced epithelial‑mesenchymal transition in human peritoneal mesothelial cells via the TGFβ/Smad3 pathway. Molecular Medicine Reports, 15, 2273-2279. https://doi.org/10.3892/mmr.2017.6276
MLA
Yang, L., Wu, L., Zhang, X., Hu, Y., Fan, Y., Ma, J."1,25(OH)2D3/VDR attenuates high glucose‑induced epithelial‑mesenchymal transition in human peritoneal mesothelial cells via the TGFβ/Smad3 pathway". Molecular Medicine Reports 15.4 (2017): 2273-2279.
Chicago
Yang, L., Wu, L., Zhang, X., Hu, Y., Fan, Y., Ma, J."1,25(OH)2D3/VDR attenuates high glucose‑induced epithelial‑mesenchymal transition in human peritoneal mesothelial cells via the TGFβ/Smad3 pathway". Molecular Medicine Reports 15, no. 4 (2017): 2273-2279. https://doi.org/10.3892/mmr.2017.6276
Copy and paste a formatted citation
x
Spandidos Publications style
Yang L, Wu L, Zhang X, Hu Y, Fan Y and Ma J: 1,25(OH)2D3/VDR attenuates high glucose‑induced epithelial‑mesenchymal transition in human peritoneal mesothelial cells via the TGFβ/Smad3 pathway. Mol Med Rep 15: 2273-2279, 2017.
APA
Yang, L., Wu, L., Zhang, X., Hu, Y., Fan, Y., & Ma, J. (2017). 1,25(OH)2D3/VDR attenuates high glucose‑induced epithelial‑mesenchymal transition in human peritoneal mesothelial cells via the TGFβ/Smad3 pathway. Molecular Medicine Reports, 15, 2273-2279. https://doi.org/10.3892/mmr.2017.6276
MLA
Yang, L., Wu, L., Zhang, X., Hu, Y., Fan, Y., Ma, J."1,25(OH)2D3/VDR attenuates high glucose‑induced epithelial‑mesenchymal transition in human peritoneal mesothelial cells via the TGFβ/Smad3 pathway". Molecular Medicine Reports 15.4 (2017): 2273-2279.
Chicago
Yang, L., Wu, L., Zhang, X., Hu, Y., Fan, Y., Ma, J."1,25(OH)2D3/VDR attenuates high glucose‑induced epithelial‑mesenchymal transition in human peritoneal mesothelial cells via the TGFβ/Smad3 pathway". Molecular Medicine Reports 15, no. 4 (2017): 2273-2279. https://doi.org/10.3892/mmr.2017.6276
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