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Article

BmK CT and 125I-BmK CT suppress the invasion of glioma cells in vitro via matrix metalloproteinase-2

  • Authors:
    • Na Sun
    • Lingzhou Zhao
    • Wenli Qiao
    • Yan Xing
    • Jinhua Zhao
  • View Affiliations / Copyright

    Affiliations: Department of Nuclear Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, P.R. China
  • Pages: 2703-2708
    |
    Published online on: March 3, 2017
       https://doi.org/10.3892/mmr.2017.6284
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Abstract

Chlorotoxin (CTX) is an established blocker of small‑conductance Cl‑ channels and has previously been demonstrated to inhibit the invasion of glioma cells. Buthus martensii Karsch chlorotoxin‑like toxin (BmK CT) is the first chlorotoxin-like peptide. The present study aimed to determine the inhibitory effect of BmK CT on the invasive ability of glioma cells, using a Transwell assay. BmK CT was subsequently radiolabeled with radionuclide 125I and its activity was compared with BmK CT. Additionally, the underlying anti‑invasive mechanism of BmK CT and 125I‑BmK CT on glioma cells was investigated by ELISA and reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). It was revealed that BmK CT and 125I‑BmK CT were able to inhibit the invasion of glioma cells and that 125I‑BmK CT was superior to BmK CT. Consistent with the results of the Transwell assay, matrix metalloproteinase‑2 (MMP‑2) secretion by glioma cells was significantly reduced following treatment with BmK CT or 125I‑BmK CT (P<0.05). However, no significant differences in MMP-2 mRNA expression levels were identified by RT‑qPCR (P>0.05). In conclusion, the present study demonstrated that BmK CT and 125I‑BmK CT reduced the invasion of glioma cells via downregulation of MMP-2 expression. However, inhibition of the invasion of glioma cells was not demonstrated at the mRNA level.
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Copy and paste a formatted citation
Spandidos Publications style
Sun N, Zhao L, Qiao W, Xing Y and Zhao J: BmK CT and 125I-BmK CT suppress the invasion of glioma cells in vitro via matrix metalloproteinase-2. Mol Med Rep 15: 2703-2708, 2017.
APA
Sun, N., Zhao, L., Qiao, W., Xing, Y., & Zhao, J. (2017). BmK CT and 125I-BmK CT suppress the invasion of glioma cells in vitro via matrix metalloproteinase-2. Molecular Medicine Reports, 15, 2703-2708. https://doi.org/10.3892/mmr.2017.6284
MLA
Sun, N., Zhao, L., Qiao, W., Xing, Y., Zhao, J."BmK CT and 125I-BmK CT suppress the invasion of glioma cells in vitro via matrix metalloproteinase-2". Molecular Medicine Reports 15.5 (2017): 2703-2708.
Chicago
Sun, N., Zhao, L., Qiao, W., Xing, Y., Zhao, J."BmK CT and 125I-BmK CT suppress the invasion of glioma cells in vitro via matrix metalloproteinase-2". Molecular Medicine Reports 15, no. 5 (2017): 2703-2708. https://doi.org/10.3892/mmr.2017.6284
Copy and paste a formatted citation
x
Spandidos Publications style
Sun N, Zhao L, Qiao W, Xing Y and Zhao J: BmK CT and 125I-BmK CT suppress the invasion of glioma cells in vitro via matrix metalloproteinase-2. Mol Med Rep 15: 2703-2708, 2017.
APA
Sun, N., Zhao, L., Qiao, W., Xing, Y., & Zhao, J. (2017). BmK CT and 125I-BmK CT suppress the invasion of glioma cells in vitro via matrix metalloproteinase-2. Molecular Medicine Reports, 15, 2703-2708. https://doi.org/10.3892/mmr.2017.6284
MLA
Sun, N., Zhao, L., Qiao, W., Xing, Y., Zhao, J."BmK CT and 125I-BmK CT suppress the invasion of glioma cells in vitro via matrix metalloproteinase-2". Molecular Medicine Reports 15.5 (2017): 2703-2708.
Chicago
Sun, N., Zhao, L., Qiao, W., Xing, Y., Zhao, J."BmK CT and 125I-BmK CT suppress the invasion of glioma cells in vitro via matrix metalloproteinase-2". Molecular Medicine Reports 15, no. 5 (2017): 2703-2708. https://doi.org/10.3892/mmr.2017.6284
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