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Negative regulation of ERK1/2 by PI3K is required for the protective effects of Pyropia yezoensis peptide against perfluorooctane sulfonate-induced endoplasmic reticulum stress

  • Authors:
    • Jeong Hwan Oh
    • Eun‑Young Kim
    • Youn Hee Choi
    • Taek‑Jeong Nam
  • View Affiliations / Copyright

    Affiliations: Cell Biology Laboratory, Institute of Fisheries Sciences, Pukyong National University, Busan 46041, Republic of Korea, Department of Marine Bio‑Materials and Aquaculture, Pukyong National University, Busan 48513, Republic of Korea
    Copyright: © Oh et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2583-2587
    |
    Published online on: March 3, 2017
       https://doi.org/10.3892/mmr.2017.6285
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Abstract

Perfluorooctane sulfonate (PFOS) is a stable fluorosurfactant, which causes adverse effects in various organisms. The present study was designed to investigate the effects of Pyropia yezoensis peptide (PYP), a peptide comprised of 11 residues (ALEGGKSSGGG), on PFOS-induced endoplasmic reticulum (ER) stress in Chang cells. PFOS exposure (400 µM) for 24 h significantly decreased cell viability, which was upregulated by 250‑1,000 pg/ml PYP treatment. Exposure to PFOS also significantly increased expression of the ER stress response protein, glucose-regulated protein 78 (GRP78), and phosphorylation of extracellular signal‑regulated kinase 1/2 (ERK1/2). These elevations were significantly decreased by PYP (250 pg/ml), and, in particular, the PFOS‑induced GRP78 upregulation was decreased following treatment with 10 µM SL327, an ERK‑kinase inhibitor. However, PYP‑induced decreases in GRP78 expression and ERK1/2 phosphorylation were upregulated following treatment with LY294002 (20 µM), a phosphatidylinositol‑3 kinase (PI3K) inhibitor. PFOS-induced apoptosis was also significantly attenuated by PYP (250 pg/ml) treatment, and the PYP‑induced reduction in apoptosis was abolished by inhibition of PI3K. These findings indicate that negative regulation of ERK1/2 by PI3K is essential for the protective effects of PYP against PFOS-induced cell death, suggesting that PYP may be a candidate for therapeutic use.
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Copy and paste a formatted citation
Spandidos Publications style
Oh JH, Kim EY, Choi YH and Nam TJ: Negative regulation of ERK1/2 by PI3K is required for the protective effects of Pyropia yezoensis peptide against perfluorooctane sulfonate-induced endoplasmic reticulum stress. Mol Med Rep 15: 2583-2587, 2017.
APA
Oh, J.H., Kim, E., Choi, Y.H., & Nam, T. (2017). Negative regulation of ERK1/2 by PI3K is required for the protective effects of Pyropia yezoensis peptide against perfluorooctane sulfonate-induced endoplasmic reticulum stress. Molecular Medicine Reports, 15, 2583-2587. https://doi.org/10.3892/mmr.2017.6285
MLA
Oh, J. H., Kim, E., Choi, Y. H., Nam, T."Negative regulation of ERK1/2 by PI3K is required for the protective effects of Pyropia yezoensis peptide against perfluorooctane sulfonate-induced endoplasmic reticulum stress". Molecular Medicine Reports 15.5 (2017): 2583-2587.
Chicago
Oh, J. H., Kim, E., Choi, Y. H., Nam, T."Negative regulation of ERK1/2 by PI3K is required for the protective effects of Pyropia yezoensis peptide against perfluorooctane sulfonate-induced endoplasmic reticulum stress". Molecular Medicine Reports 15, no. 5 (2017): 2583-2587. https://doi.org/10.3892/mmr.2017.6285
Copy and paste a formatted citation
x
Spandidos Publications style
Oh JH, Kim EY, Choi YH and Nam TJ: Negative regulation of ERK1/2 by PI3K is required for the protective effects of Pyropia yezoensis peptide against perfluorooctane sulfonate-induced endoplasmic reticulum stress. Mol Med Rep 15: 2583-2587, 2017.
APA
Oh, J.H., Kim, E., Choi, Y.H., & Nam, T. (2017). Negative regulation of ERK1/2 by PI3K is required for the protective effects of Pyropia yezoensis peptide against perfluorooctane sulfonate-induced endoplasmic reticulum stress. Molecular Medicine Reports, 15, 2583-2587. https://doi.org/10.3892/mmr.2017.6285
MLA
Oh, J. H., Kim, E., Choi, Y. H., Nam, T."Negative regulation of ERK1/2 by PI3K is required for the protective effects of Pyropia yezoensis peptide against perfluorooctane sulfonate-induced endoplasmic reticulum stress". Molecular Medicine Reports 15.5 (2017): 2583-2587.
Chicago
Oh, J. H., Kim, E., Choi, Y. H., Nam, T."Negative regulation of ERK1/2 by PI3K is required for the protective effects of Pyropia yezoensis peptide against perfluorooctane sulfonate-induced endoplasmic reticulum stress". Molecular Medicine Reports 15, no. 5 (2017): 2583-2587. https://doi.org/10.3892/mmr.2017.6285
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