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High mobility group box 1 induces calcification of aortic valve interstitial cells via toll-like receptor 4

  • Authors:
    • Wenjun Shen
    • Jianqing Zhou
    • Chaoyang Wang
    • Guangze Xu
    • Ying Wu
    • Zhaohui Hu
  • View Affiliations / Copyright

    Affiliations: Ningbo Medical Treatment Center, Lihuili Hospital, Ningbo, Zhejiang 310041, P.R. China, Department of Cardiovascular Disease, The Affiliated Tongji Hospital, Tongji University, Shanghai 210062, P.R. China
    Copyright: © Shen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2530-2536
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    Published online on: March 3, 2017
       https://doi.org/10.3892/mmr.2017.6287
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Abstract

Chronic inflammation and the calcification of aortic valve interstitial cells (AVICs) are the primary etiologies of calcific aortic valve disease (CAVD). However, the underlying mechanism remains to be elucidated. The present study investigated the importance of high mobility group box 1 (HMGB1) via toll‑like receptor 4 (TLR4) for the regulation of inflammation and calcification in AVICs. It was determined that the expression levels of HMGB1 and TLR4 were increased in the calcific region of aortic valves with CAVD. In cultured primary AVICs from wild‑type mice, HMGB1 treatment demonstrated a dose-dependent increase in mineralization levels and osteogenic gene expression. These effects were significantly reduced in AVICs obtained from TLR4 knockout mice (TLR4‑/‑). In addition, calcification was inhibited by TLR4-specific antibodies in primary AVICs. HMGB1 induced the activation of p38 and nuclear factor-κB (NF-κB) in TLR4‑/‑ primary AVICs, and inhibited p38 and NF‑κB in wild‑type AVICs treated with TLR4‑specific antibodies. The present study demonstrated that TLR4 may function as an essential mediator of HMGB1‑induced calcification and in the activation of p38 and NF-κB.
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Copy and paste a formatted citation
Spandidos Publications style
Shen W, Zhou J, Wang C, Xu G, Wu Y and Hu Z: High mobility group box 1 induces calcification of aortic valve interstitial cells via toll-like receptor 4. Mol Med Rep 15: 2530-2536, 2017.
APA
Shen, W., Zhou, J., Wang, C., Xu, G., Wu, Y., & Hu, Z. (2017). High mobility group box 1 induces calcification of aortic valve interstitial cells via toll-like receptor 4. Molecular Medicine Reports, 15, 2530-2536. https://doi.org/10.3892/mmr.2017.6287
MLA
Shen, W., Zhou, J., Wang, C., Xu, G., Wu, Y., Hu, Z."High mobility group box 1 induces calcification of aortic valve interstitial cells via toll-like receptor 4". Molecular Medicine Reports 15.5 (2017): 2530-2536.
Chicago
Shen, W., Zhou, J., Wang, C., Xu, G., Wu, Y., Hu, Z."High mobility group box 1 induces calcification of aortic valve interstitial cells via toll-like receptor 4". Molecular Medicine Reports 15, no. 5 (2017): 2530-2536. https://doi.org/10.3892/mmr.2017.6287
Copy and paste a formatted citation
x
Spandidos Publications style
Shen W, Zhou J, Wang C, Xu G, Wu Y and Hu Z: High mobility group box 1 induces calcification of aortic valve interstitial cells via toll-like receptor 4. Mol Med Rep 15: 2530-2536, 2017.
APA
Shen, W., Zhou, J., Wang, C., Xu, G., Wu, Y., & Hu, Z. (2017). High mobility group box 1 induces calcification of aortic valve interstitial cells via toll-like receptor 4. Molecular Medicine Reports, 15, 2530-2536. https://doi.org/10.3892/mmr.2017.6287
MLA
Shen, W., Zhou, J., Wang, C., Xu, G., Wu, Y., Hu, Z."High mobility group box 1 induces calcification of aortic valve interstitial cells via toll-like receptor 4". Molecular Medicine Reports 15.5 (2017): 2530-2536.
Chicago
Shen, W., Zhou, J., Wang, C., Xu, G., Wu, Y., Hu, Z."High mobility group box 1 induces calcification of aortic valve interstitial cells via toll-like receptor 4". Molecular Medicine Reports 15, no. 5 (2017): 2530-2536. https://doi.org/10.3892/mmr.2017.6287
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