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Article

Repression of the expression of TET2 by ZEB1 contributes to invasion and growth in glioma cells

  • Authors:
    • Bo Chen
    • Yanqing Lei
    • Hongquan Wang
    • Yanwei Dang
    • Peihai Fang
    • Jun Wang
    • Jinbo Yang
    • Lijun Liu
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, Xiangyang No. 1 People's Hospital, Hubei University of Medicine, Xiangyang, Hubei 441000, P.R. China, Department of Emergency, Xiangyang No. 1 People's Hospital, Hubei University of Medicine, Xiangyang, Hubei 441000, P.R. China
  • Pages: 2625-2632
    |
    Published online on: March 3, 2017
       https://doi.org/10.3892/mmr.2017.6288
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Abstract

Malignant gliomas are the most common and aggressive type of brain tumor. The suppressive role of ten-eleven translocation 2 (TET2) has been implicated in certain types of cancer, however, its role in gliomas remains to be elucidated. The present study aimed to determine the expression pattern and biological role of TET2 in glioma, using RT-qPCR and immunohistochemistry, and its results indicated that the expression of TET2 was frequently decreased in gliomas and that repression of the expression of TET2 correlated with the progression of glioma. The ectopic expression of TET2 inhibited the invasive potential of glioma cells, and inhibited glioma cell proliferation in vitro and growth in vivo. Additionally, the expression of Zinc finger E‑box‑binding homeobox 1 (ZEB1) was increased in gliomas and was positively correlated with progression, but inversely correlated with the expression of TET2. ZEB1 was also confirmed to physically bind to the TET2 promoter. ZEB1 knockdown resulted in an increase in the expression of TET2 and elevation of TET2 promoter activity in glioma cells. These findings indicated that the downregulation of TET2 by ZEB1 is a critical oncogenic event in gliomas.
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Copy and paste a formatted citation
Spandidos Publications style
Chen B, Lei Y, Wang H, Dang Y, Fang P, Wang J, Yang J and Liu L: Repression of the expression of TET2 by ZEB1 contributes to invasion and growth in glioma cells. Mol Med Rep 15: 2625-2632, 2017.
APA
Chen, B., Lei, Y., Wang, H., Dang, Y., Fang, P., Wang, J. ... Liu, L. (2017). Repression of the expression of TET2 by ZEB1 contributes to invasion and growth in glioma cells. Molecular Medicine Reports, 15, 2625-2632. https://doi.org/10.3892/mmr.2017.6288
MLA
Chen, B., Lei, Y., Wang, H., Dang, Y., Fang, P., Wang, J., Yang, J., Liu, L."Repression of the expression of TET2 by ZEB1 contributes to invasion and growth in glioma cells". Molecular Medicine Reports 15.5 (2017): 2625-2632.
Chicago
Chen, B., Lei, Y., Wang, H., Dang, Y., Fang, P., Wang, J., Yang, J., Liu, L."Repression of the expression of TET2 by ZEB1 contributes to invasion and growth in glioma cells". Molecular Medicine Reports 15, no. 5 (2017): 2625-2632. https://doi.org/10.3892/mmr.2017.6288
Copy and paste a formatted citation
x
Spandidos Publications style
Chen B, Lei Y, Wang H, Dang Y, Fang P, Wang J, Yang J and Liu L: Repression of the expression of TET2 by ZEB1 contributes to invasion and growth in glioma cells. Mol Med Rep 15: 2625-2632, 2017.
APA
Chen, B., Lei, Y., Wang, H., Dang, Y., Fang, P., Wang, J. ... Liu, L. (2017). Repression of the expression of TET2 by ZEB1 contributes to invasion and growth in glioma cells. Molecular Medicine Reports, 15, 2625-2632. https://doi.org/10.3892/mmr.2017.6288
MLA
Chen, B., Lei, Y., Wang, H., Dang, Y., Fang, P., Wang, J., Yang, J., Liu, L."Repression of the expression of TET2 by ZEB1 contributes to invasion and growth in glioma cells". Molecular Medicine Reports 15.5 (2017): 2625-2632.
Chicago
Chen, B., Lei, Y., Wang, H., Dang, Y., Fang, P., Wang, J., Yang, J., Liu, L."Repression of the expression of TET2 by ZEB1 contributes to invasion and growth in glioma cells". Molecular Medicine Reports 15, no. 5 (2017): 2625-2632. https://doi.org/10.3892/mmr.2017.6288
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