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Article

miR-21 inhibitor suppresses cell proliferation and colony formation through regulating the PTEN/AKT pathway and improves paclitaxel sensitivity in cervical cancer cells

  • Authors:
    • Guohui Du
    • Dongmei Cao
    • Lingzheng Meng
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacy, Tangshan Maternity and Child Health Care Hospital, Tangshan, Hebei 063000, P.R. China
  • Pages: 2713-2719
    |
    Published online on: March 16, 2017
       https://doi.org/10.3892/mmr.2017.6340
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Abstract

The present study aimed to investigate the role and the molecular mechanisms underlying the effects of microRNA-21 (miR-21) on the proliferation, apoptosis and colony formation of cervical cancer cells, and to examine the role of miR-21 in mediating the sensitivity of cervical cancer cells to paclitaxel (PTX). Reverse transcription‑quantitative polymerase chain reaction was employed to determine the level of miR‑21 in various cervical cancer and normal cervical cells. The results revealed that the expression levels of miR-21 in cervical cancer cells were markedly higher when compared with normal cervical cells. Subsequently, a miR‑21 inhibitor or negative control (NC) was transfected into cervical cancer cells. Cell viability, colony formation and apoptosis were then analyzed using an MTT assay, crystal violet and Annexin V-fluorescein isothiocyanate/propidium iodide staining, respectively. The protein expression level of B-cell lymphoma‑2 (Bcl‑2), Bcl‑2‑associated X (Bax), programmed cell death 4 (PDCD4), survivin, c‑myc, phosphatase and tensin homolog (PTEN) and phosphorylated (p)‑AKT were determined by western blot analysis. The sensitivity of cervical cancer cells to PTX (25, 50 and 100 µg/ml) was characterized using an MTT assay. The results demonstrated that the miR-21 inhibitor promoted apoptosis of cervical cancer cells and suppressed their proliferation and colony formation when compared with the NC. In addition, the expression levels of Bcl‑2, survivin, c‑myc and p‑AKT were significantly downregulated in cells transfected with the miR‑21 inhibitor, whilst the expression levels of Bax, PDCD4 and PTEN were significantly upregulated. Furthermore, the miR‑21 inhibitor significantly enhanced the inhibition efficacy of PTX at a range of concentrations in cervical cancer cells. It was concluded that inhibition of miR‑21 suppressed cell proliferation and colony formation through regulating the PTEN/AKT pathway, and improved PTX sensitivity in cervical cancer cells. The results of the present study may contribute to the development of miRNA‑based cervical cancer therapy in the future.
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Copy and paste a formatted citation
Spandidos Publications style
Du G, Cao D and Meng L: miR-21 inhibitor suppresses cell proliferation and colony formation through regulating the PTEN/AKT pathway and improves paclitaxel sensitivity in cervical cancer cells. Mol Med Rep 15: 2713-2719, 2017.
APA
Du, G., Cao, D., & Meng, L. (2017). miR-21 inhibitor suppresses cell proliferation and colony formation through regulating the PTEN/AKT pathway and improves paclitaxel sensitivity in cervical cancer cells. Molecular Medicine Reports, 15, 2713-2719. https://doi.org/10.3892/mmr.2017.6340
MLA
Du, G., Cao, D., Meng, L."miR-21 inhibitor suppresses cell proliferation and colony formation through regulating the PTEN/AKT pathway and improves paclitaxel sensitivity in cervical cancer cells". Molecular Medicine Reports 15.5 (2017): 2713-2719.
Chicago
Du, G., Cao, D., Meng, L."miR-21 inhibitor suppresses cell proliferation and colony formation through regulating the PTEN/AKT pathway and improves paclitaxel sensitivity in cervical cancer cells". Molecular Medicine Reports 15, no. 5 (2017): 2713-2719. https://doi.org/10.3892/mmr.2017.6340
Copy and paste a formatted citation
x
Spandidos Publications style
Du G, Cao D and Meng L: miR-21 inhibitor suppresses cell proliferation and colony formation through regulating the PTEN/AKT pathway and improves paclitaxel sensitivity in cervical cancer cells. Mol Med Rep 15: 2713-2719, 2017.
APA
Du, G., Cao, D., & Meng, L. (2017). miR-21 inhibitor suppresses cell proliferation and colony formation through regulating the PTEN/AKT pathway and improves paclitaxel sensitivity in cervical cancer cells. Molecular Medicine Reports, 15, 2713-2719. https://doi.org/10.3892/mmr.2017.6340
MLA
Du, G., Cao, D., Meng, L."miR-21 inhibitor suppresses cell proliferation and colony formation through regulating the PTEN/AKT pathway and improves paclitaxel sensitivity in cervical cancer cells". Molecular Medicine Reports 15.5 (2017): 2713-2719.
Chicago
Du, G., Cao, D., Meng, L."miR-21 inhibitor suppresses cell proliferation and colony formation through regulating the PTEN/AKT pathway and improves paclitaxel sensitivity in cervical cancer cells". Molecular Medicine Reports 15, no. 5 (2017): 2713-2719. https://doi.org/10.3892/mmr.2017.6340
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