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Article Open Access

Islet-1 induces the differentiation of mesenchymal stem cells into cardiomyocyte-like cells through the regulation of Gcn5 and DNMT-1

  • Authors:
    • Qin Yi
    • Hao Xu
    • Ke Yang
    • Yue Wang
    • Bin Tan
    • Jie Tian
    • Jing Zhu
  • View Affiliations / Copyright

    Affiliations: Department of Pediatric Research Institute, Children's Hospital of Chongqing Medical University, Chongqing 400014, P.R. China, Cardiovascular Department (Internal Medicine), Children's Hospital of Chongqing Medical University, Chongqing 400014, P.R. China
    Copyright: © Yi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2511-2520
    |
    Published online on: March 16, 2017
       https://doi.org/10.3892/mmr.2017.6343
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Abstract

Previous studies from this group demonstrated that insulin gene enhancer binding protein ISL-1 (Islet-1) specifically induces the differentiation of mesenchymal stem cells (MSCs) into cardiomyocyte‑like cells through histone acetylation. However, the underlying mechanisms remain unclear. In the present study, the role of the histone acetylation and DNA methylation on the regulatory mechanism of the Islet‑1 was further investigated by methylation‑specific polymerase chain reaction (PCR), chromatin immunoprecipitation quantitative PCR and western blot analysis. The results demonstrated that Islet‑1 upregulated expression of general control of amino acid biosynthesis protein 5 (Gcn5) and enhanced the binding of Gcn5 to the promoters of GATA binding protein 4 (GATA4) and NK2 homeobox 5 (Nkx2.5). In addition, Islet-1 downregulated DNA methyltransferase (DNMT)‑1 expression and reduced its binding to the GATA4 promoter. In contrast, the amount of DNMT-1 binding on Nkx2.5 did not match the expression trend. Therefore, it was concluded that Islet‑1 may influence the histone acetylation and DNA methylation of GATA4 promoter region via Gcn5 and DNMT‑1 during the MSC differentiation into cardiomyocyte-like cells, thus prompting the expression of GATA4. The Nkx2.5 was likely only affected by histone acetylation instead of DNA methylation. The present study demonstrated that Islet‑1 induces the differentiation of mesenchymal stem cells into cardiomyocyte‑like cells through a specific interaction between histone acetylation and DNA methylation on regulating GATA4.
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Copy and paste a formatted citation
Spandidos Publications style
Yi Q, Xu H, Yang K, Wang Y, Tan B, Tian J and Zhu J: Islet-1 induces the differentiation of mesenchymal stem cells into cardiomyocyte-like cells through the regulation of Gcn5 and DNMT-1. Mol Med Rep 15: 2511-2520, 2017.
APA
Yi, Q., Xu, H., Yang, K., Wang, Y., Tan, B., Tian, J., & Zhu, J. (2017). Islet-1 induces the differentiation of mesenchymal stem cells into cardiomyocyte-like cells through the regulation of Gcn5 and DNMT-1. Molecular Medicine Reports, 15, 2511-2520. https://doi.org/10.3892/mmr.2017.6343
MLA
Yi, Q., Xu, H., Yang, K., Wang, Y., Tan, B., Tian, J., Zhu, J."Islet-1 induces the differentiation of mesenchymal stem cells into cardiomyocyte-like cells through the regulation of Gcn5 and DNMT-1". Molecular Medicine Reports 15.5 (2017): 2511-2520.
Chicago
Yi, Q., Xu, H., Yang, K., Wang, Y., Tan, B., Tian, J., Zhu, J."Islet-1 induces the differentiation of mesenchymal stem cells into cardiomyocyte-like cells through the regulation of Gcn5 and DNMT-1". Molecular Medicine Reports 15, no. 5 (2017): 2511-2520. https://doi.org/10.3892/mmr.2017.6343
Copy and paste a formatted citation
x
Spandidos Publications style
Yi Q, Xu H, Yang K, Wang Y, Tan B, Tian J and Zhu J: Islet-1 induces the differentiation of mesenchymal stem cells into cardiomyocyte-like cells through the regulation of Gcn5 and DNMT-1. Mol Med Rep 15: 2511-2520, 2017.
APA
Yi, Q., Xu, H., Yang, K., Wang, Y., Tan, B., Tian, J., & Zhu, J. (2017). Islet-1 induces the differentiation of mesenchymal stem cells into cardiomyocyte-like cells through the regulation of Gcn5 and DNMT-1. Molecular Medicine Reports, 15, 2511-2520. https://doi.org/10.3892/mmr.2017.6343
MLA
Yi, Q., Xu, H., Yang, K., Wang, Y., Tan, B., Tian, J., Zhu, J."Islet-1 induces the differentiation of mesenchymal stem cells into cardiomyocyte-like cells through the regulation of Gcn5 and DNMT-1". Molecular Medicine Reports 15.5 (2017): 2511-2520.
Chicago
Yi, Q., Xu, H., Yang, K., Wang, Y., Tan, B., Tian, J., Zhu, J."Islet-1 induces the differentiation of mesenchymal stem cells into cardiomyocyte-like cells through the regulation of Gcn5 and DNMT-1". Molecular Medicine Reports 15, no. 5 (2017): 2511-2520. https://doi.org/10.3892/mmr.2017.6343
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