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Article

Chronological changes in the expression of phosphorylated tau and 5‑AMP‑activated protein kinase in the brain of senescence‑accelerated P8 mice

  • Authors:
    • Hak‑Su Kim
    • Sohee Moon
    • Sujin Kim
    • Min‑Jae Lee
    • Min Hwa Suk
    • Dong‑Ho Park
    • Dong Wun Shin
    • Chang‑Shin Park
    • Ju‑Hee Kang
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology and Medicinal Toxicology Research Center, Inha University School of Medicine, Inha University, Incheon 22212, Republic of Korea, Department of Kinesiology, College of Arts and Sports, Inha University, Incheon 22212, Republic of Korea, Department of Physical Education, College of Education, Seoul National University, Seoul 08826, Republic of Korea, Department of Emergency Medicine, Inje University Ilsan Paik Hospital, Goyang, Gyeonggi 10380, Republic of Korea
  • Pages: 3301-3309
    |
    Published online on: March 23, 2017
       https://doi.org/10.3892/mmr.2017.6361
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Abstract

Senescence-accelerated mouse prone 8 (SAMP8), a non‑transgenic animal model used for researching sporadic Alzheimer's disease (AD), presents AD pathologies and overall dysregulation in brain energy metabolism, which is one of the early pathogenic characteristics of AD. In the present study, the authors examined chronological changes in the expression patterns of phosphorylated tau and of proteins related to energy metabolism to evaluate the association of tau phosphorylation and metabolism, using young‑ (2‑months‑old), middle‑ (5‑months‑old) and old‑aged (10‑months‑old) SAMP8. The levels of phosphorylated 5'‑AMP activated protein kinase at Thr172 (p‑AMPK) and phosphorylated glycogen synthase kinase 3β (p‑GSK3βS9) in the cortex of SAMP8 at 2 months were significantly higher than those in senescence‑accelerated mouse resistant 1 (SAMR1). The differences were not detected at 5 and 10 months of age, which were concurrent with the changes in levels of phosphorylated tau at Ser396 (p‑tauS396), but not with p‑tauS262. The level of p‑tauS262 was considerably higher in the cortex of middle‑aged SAMP8 when compared with that of SAMR1 and sustained in old‑aged SAMP8, but not in the young cortex. The levels of cortical sirtuin1 (Sirt1) and insulin receptor substrate 1 (IRS‑1) expression of young SAMP8 were significantly lower, when compared with those in SAMR1. However, in the hippocampus of SAMP8, the patterns of chronological changes and levels of p‑tau, p‑AMPK, Sirt1 and IRS‑1 relative to SAMR1 were different from those in the cortex. Taken together, the results suggested that regulation of tau phosphorylation via the AMPK‑GSK3β pathway concurrent with dysregulation of energy metabolism may precede the pathological tau hyperphosphorylation in the cortex of SAMP8, and that the regulation of AMPK‑GSK3β‑mediated tau phosphorylation may be dependent on phosphor‑epitope in tau or the region of brain.
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Copy and paste a formatted citation
Spandidos Publications style
Kim HS, Moon S, Kim S, Lee MJ, Suk MH, Park DH, Shin DW, Park CS and Kang JH: Chronological changes in the expression of phosphorylated tau and 5‑AMP‑activated protein kinase in the brain of senescence‑accelerated P8 mice. Mol Med Rep 15: 3301-3309, 2017.
APA
Kim, H., Moon, S., Kim, S., Lee, M., Suk, M.H., Park, D. ... Kang, J. (2017). Chronological changes in the expression of phosphorylated tau and 5‑AMP‑activated protein kinase in the brain of senescence‑accelerated P8 mice. Molecular Medicine Reports, 15, 3301-3309. https://doi.org/10.3892/mmr.2017.6361
MLA
Kim, H., Moon, S., Kim, S., Lee, M., Suk, M. H., Park, D., Shin, D. W., Park, C., Kang, J."Chronological changes in the expression of phosphorylated tau and 5‑AMP‑activated protein kinase in the brain of senescence‑accelerated P8 mice". Molecular Medicine Reports 15.5 (2017): 3301-3309.
Chicago
Kim, H., Moon, S., Kim, S., Lee, M., Suk, M. H., Park, D., Shin, D. W., Park, C., Kang, J."Chronological changes in the expression of phosphorylated tau and 5‑AMP‑activated protein kinase in the brain of senescence‑accelerated P8 mice". Molecular Medicine Reports 15, no. 5 (2017): 3301-3309. https://doi.org/10.3892/mmr.2017.6361
Copy and paste a formatted citation
x
Spandidos Publications style
Kim HS, Moon S, Kim S, Lee MJ, Suk MH, Park DH, Shin DW, Park CS and Kang JH: Chronological changes in the expression of phosphorylated tau and 5‑AMP‑activated protein kinase in the brain of senescence‑accelerated P8 mice. Mol Med Rep 15: 3301-3309, 2017.
APA
Kim, H., Moon, S., Kim, S., Lee, M., Suk, M.H., Park, D. ... Kang, J. (2017). Chronological changes in the expression of phosphorylated tau and 5‑AMP‑activated protein kinase in the brain of senescence‑accelerated P8 mice. Molecular Medicine Reports, 15, 3301-3309. https://doi.org/10.3892/mmr.2017.6361
MLA
Kim, H., Moon, S., Kim, S., Lee, M., Suk, M. H., Park, D., Shin, D. W., Park, C., Kang, J."Chronological changes in the expression of phosphorylated tau and 5‑AMP‑activated protein kinase in the brain of senescence‑accelerated P8 mice". Molecular Medicine Reports 15.5 (2017): 3301-3309.
Chicago
Kim, H., Moon, S., Kim, S., Lee, M., Suk, M. H., Park, D., Shin, D. W., Park, C., Kang, J."Chronological changes in the expression of phosphorylated tau and 5‑AMP‑activated protein kinase in the brain of senescence‑accelerated P8 mice". Molecular Medicine Reports 15, no. 5 (2017): 3301-3309. https://doi.org/10.3892/mmr.2017.6361
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