Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
May-2017 Volume 15 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
May-2017 Volume 15 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Role of tropomyosin in silkworm allergy

  • Authors:
    • Kyoung Yong Jeong
    • In‑Soo Han
    • June Yong Lee
    • Kyung Hee Park
    • Jae‑Hyun Lee
    • Jung‑Won Park
  • View Affiliations / Copyright

    Affiliations: Department of Internal Medicine, Institute of Allergy, Yonsei University College of Medicine, Seoul 03722, Republic of Korea
  • Pages: 3264-3270
    |
    Published online on: March 24, 2017
       https://doi.org/10.3892/mmr.2017.6373
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Silkworm pupae are widely consumed in Asian countries and allergic reactions following consumption have been described. However, false‑positive responses in skin prick allergy tests or non‑specific immunoglobulin E (IgE) responses to total extract of silkworm pupa make diagnosis difficult. Although improved allergy diagnosis is required, molecular characterization of silkworm allergens has not been performed to date, except for Bomb m 1, an arginine kinase. This study aimed to evaluate the allergenicity of tropomyosin, a well‑established invertebrate pan‑allergen, from silkworm pupa. The silkworm tropomyosin gene was cloned by reverse transcription and polymerase chain reaction, and the protein was overexpressed in Escherichia coli and purified by affinity chromatography using Nickel‑resin. IgE reactivity of the recombinant protein was examined by ELISA and competitive inhibition analyses. Silkworm pupa tropomyosin shared 73.5‑92.3% amino acid sequence identity with previously identified allergenic tropomyosins. Sera from eight of 15 patients with silkworm allergy (53.3%) exhibited binding of IgE to the recombinant protein. However, recombinant protein was able to inhibit less than 10% of IgE reactivity to silkworm pupa extract. Of the eight sera tested, six that specifically reacted with silkworm tropomyosin also demonstrated IgE reactivity to shrimp and crab. In the present study, specific IgE to silkworm tropomyosin was detected in patients with silkworm allergy, suggesting that it may be useful in diagnosis of allergy to silkworm pupa.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

DeFoliart GR: Insects as food: Why the Western attitude is important. Annu Rev Entomol. 44:21–50. 1999. View Article : Google Scholar : PubMed/NCBI

2 

Pemberton RW: Insect and other arthropods used as drugs in Korean traditional medicine. J Ethnopharmacol. 65:207–216. 1999. View Article : Google Scholar : PubMed/NCBI

3 

Ramos-Elorduy J: Insects: A sustainable source of food? Ecol Food Nutr. 36:247–276. 1997. View Article : Google Scholar

4 

Ji KM, Zhan ZK, Chen JJ and Liu ZG: Anaphylactic shock caused by silkworm pupa consumption in China. Allergy. 63:1407–1408. 2008. View Article : Google Scholar : PubMed/NCBI

5 

Kim SH, Kang HR, Kim KM, Kim TB, Kim SS, Chang YS, Kim CW, Bahn JW, Kim YK, Cho SH, et al: The sensitization rates of food allergens in a Korean population: A multi-center study. J Asthma Allergy Clin Immunol. 23:502–514. 2003.

6 

Komaze Y, Sakata M, Azuma T, Tanaka A and Nakagawa T: IgE antibodies against midge and moth found in Japanese asthmatic subjects and comparison of allergenicity between these insects. Allergy. 52:75–81. 1997. View Article : Google Scholar : PubMed/NCBI

7 

Baldo BA and Panzani RC: Detection of IgE antibodies to a wide range of insect species in subjects with suspected inhalant allergies to insects. Int Arch Allergy Appl Immunol. 85:278–287. 1988. View Article : Google Scholar : PubMed/NCBI

8 

Jeong KY, Hong CS and Yong TS: Allergenic tropomyosins and their cross-reactivities. Protein Pept Lett. 13:835–845. 2006. View Article : Google Scholar : PubMed/NCBI

9 

Altschul SF, Gish W, Miller W, Myers EW and Lipman DJ: Basic local alignment search tool. J Mol Bio. 215:403–410. 1990. View Article : Google Scholar

10 

Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, et al: Clustal W and Clustal X version 2.0. Bioinformatics. 23:2947–2948. 2007. View Article : Google Scholar : PubMed/NCBI

11 

Jeong KY, Son M, Lee JY, Park KH, Lee JH and Park JW: Allergenic characterization of 27-kDa glycoprotein, a novel heat stable allergen, from the pupa of silkworm, Bombyx mori. J Korean Med Sci. 31:18–24. 2016. View Article : Google Scholar : PubMed/NCBI

12 

Jeong KY, Lee J, Lee IY, Ree HI, Hong CS and Yong TS: Allergenicity of recombinant Bla g 7, German cockroach tropomyosin. Allergy. 58:1059–1063. 2003. View Article : Google Scholar : PubMed/NCBI

13 

Jeong KY: Characterization of allergenic properties of German cockroach tropomyosin using recombinant proteins, Yonsei University College of Medicine. 2004.

14 

Lee BW, Shek LP, Gerez IF, Soh SE and Van Bever HP: Food allergy-lessons from Asia. World Allergy Organ J. 1:129–133. 2008. View Article : Google Scholar : PubMed/NCBI

15 

Leung NY, Wai CY, Chu S, Wang J, Kenny TP, Chu KH and Leung PS: Current immunological and molecular biological perspectives on seafood allergy: A comprehensive review. Clin Rev Allergy Immunol. 46:180–197. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Lee MH, Kim YY and Kang SY: Two cases of pupa allergy. Allergy. 2:23–25. 1982.

17 

Kim S, Paik SW and Kang SY: A case of anaphylaxis after ingestion of pupae. Allergy. 3:175–178. 1983.

18 

Koh YI, Choi IS, Chung SU and Cho S: Clinical features of adult patients with anaphylaxis associated with food in Gwangju and Chonnam area. J Asthma Allergy Clin Immunol. 24:217–223. 2004.

19 

Jeong KY, Lee H, Lee JS, Lee J, Lee IY, Ree HI, Hong CS and Yong TS: Molecular cloning and the allergenic characterization of tropomyosin from Tyrophagus putrescentiae. Protein Pept Lett. 14:431–436. 2007. View Article : Google Scholar : PubMed/NCBI

20 

Jeong KY, Yum HY, Lee IY, Ree HI, Hong CS, Kim DS and Yong TS: Molecular cloning and characterization of tropomyosin, a major allergen of Chironomus kiiensis, a dominant species of nonbiting midges in Korea. Clin Diagn Lab Immunol. 11:320–324. 2004.PubMed/NCBI

21 

Srinroch C, Srisomsap C, Chokchaichamnankit D, Punyarit P and Phiriyangkul P: Identification of novel allergen in edible insect, Gryllus bimaculatus and its cross-reactivity with Macrobrachium spp. Allergens. Food Chem. 184:160–166. 2015. View Article : Google Scholar : PubMed/NCBI

22 

Liu Z, Xia L, Wu Y, Xia Q, Chen J and Roux KH: Identification and characterization of an arginine kinase as a major allergen from silkworm (Bombyx mori) larvae. Int Arch Allergy Immunol. 150:8–14. 2009. View Article : Google Scholar : PubMed/NCBI

23 

Zhao X, Li L, Kuang Z, Luo G and Li B: Proteomic and immunological identification of two new allergens from silkworm (Bombyx mori L.) pupae. Cent Eur J Immunol. 40:30–34. 2015. View Article : Google Scholar : PubMed/NCBI

24 

Zuo J, Lei M, Yang R and Liu Z: Bom m 9 from Bombyx mori is a novel protein related to asthma. Microbiol Immunol. 59:410–418. 2015. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Jeong KY, Han IS, Lee JY, Park KH, Lee JH and Park JW: Role of tropomyosin in silkworm allergy. Mol Med Rep 15: 3264-3270, 2017.
APA
Jeong, K.Y., Han, I., Lee, J.Y., Park, K.H., Lee, J., & Park, J. (2017). Role of tropomyosin in silkworm allergy. Molecular Medicine Reports, 15, 3264-3270. https://doi.org/10.3892/mmr.2017.6373
MLA
Jeong, K. Y., Han, I., Lee, J. Y., Park, K. H., Lee, J., Park, J."Role of tropomyosin in silkworm allergy". Molecular Medicine Reports 15.5 (2017): 3264-3270.
Chicago
Jeong, K. Y., Han, I., Lee, J. Y., Park, K. H., Lee, J., Park, J."Role of tropomyosin in silkworm allergy". Molecular Medicine Reports 15, no. 5 (2017): 3264-3270. https://doi.org/10.3892/mmr.2017.6373
Copy and paste a formatted citation
x
Spandidos Publications style
Jeong KY, Han IS, Lee JY, Park KH, Lee JH and Park JW: Role of tropomyosin in silkworm allergy. Mol Med Rep 15: 3264-3270, 2017.
APA
Jeong, K.Y., Han, I., Lee, J.Y., Park, K.H., Lee, J., & Park, J. (2017). Role of tropomyosin in silkworm allergy. Molecular Medicine Reports, 15, 3264-3270. https://doi.org/10.3892/mmr.2017.6373
MLA
Jeong, K. Y., Han, I., Lee, J. Y., Park, K. H., Lee, J., Park, J."Role of tropomyosin in silkworm allergy". Molecular Medicine Reports 15.5 (2017): 3264-3270.
Chicago
Jeong, K. Y., Han, I., Lee, J. Y., Park, K. H., Lee, J., Park, J."Role of tropomyosin in silkworm allergy". Molecular Medicine Reports 15, no. 5 (2017): 3264-3270. https://doi.org/10.3892/mmr.2017.6373
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team