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B(a)P adduct levels and fertility: A cross‑sectional study in a Sicilian population

  • Authors:
    • Gea Oliveri Conti
    • Aldo Eugenio Calogero
    • Filippo Giacone
    • Maria Fiore
    • Martina Barchitta
    • Antonella Agodi
    • Margherita Ferrante
  • View Affiliations / Copyright

    Affiliations: Environmental and Food Hygiene Laboratories, Department of Medical, Surgical Sciences and Advanced Technologies ‘G.F. Ingrassia’, University of Catania, I‑95123 Catania, Italy, Department of Clinical and Experimental Medicine, Surgical Sciences and Advanced Technologies ‘G.F. Ingrassia’, University of Catania, I‑95123 Catania, Italy, Department of Medical, Surgical Sciences and Advanced Technologies ‘G.F. Ingrassia’, University of Catania, I‑95123 Catania, Italy
    Copyright: © Oliveri Conti et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3398-3404
    |
    Published online on: March 27, 2017
       https://doi.org/10.3892/mmr.2017.6396
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Abstract

Benzo(a)pyrene (BaP) is a carcinogenic polycyclic aromatic hydrocarbon for human tissues. Still today it is not fully investigated if BaP can affect negatively the male fertility through the BaP‑DNA adducts production. In the present study, BaP Tetrol I‑1 (TI‑1) and BaP Tetrol II‑2 (TII‑2) BaP‑DNA adducts were investigated in spermatozoa of a Sicilian male population. Semen samples from 86 volunteers in two eastern Sicilian cities (Regalbuto and Melilli) were collected. The quality of semen was evaluated in all samples according to the World Health Organization (WHO) guidelines. We analyzed BaP‑DNA adducts in extracted sperm cell DNA using the modified high‑performance liquid chromatography‑fluorescence method to detects both Tetrols. Differences between Tetrol levels were assessed by the Wilcoxon signed‑rank test and the Mann‑Whitney U test, as appropriate. Correlation between semen quality parameters and Tetrol concentrations were analyzed using the Spearman's correlation coefficient. Σ(TI‑1+TII‑2) were significantly higher in spermatozoa of volunteers from Regalbuto. Furthermore, a greater dispersion of the levels of adducts was observed in these specimens. TI‑1 adducts were higher than TII‑2 in Melilli samples (95% CI) and TII‑2 were higher than TI‑1 in Regalbuto semen samples (95% CI). A significant inverse correlation between sperm progressive motility and both TI‑1 and TII‑2 adducts was observed. The present study showed that BaP negatively affects male fertility by TI‑1 and TII‑2 DNA‑adduct production. These results suggest that DNA adducts could be used as biomarker to assess BaP exposure by air pollution. Further studies are needed to confirm if these findings could affect male fertility because of the growing impairment of this function observed in recent years.
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Copy and paste a formatted citation
Spandidos Publications style
Oliveri Conti G, Calogero AE, Giacone F, Fiore M, Barchitta M, Agodi A and Ferrante M: B(a)P adduct levels and fertility: A cross‑sectional study in a Sicilian population. Mol Med Rep 15: 3398-3404, 2017.
APA
Oliveri Conti, G., Calogero, A.E., Giacone, F., Fiore, M., Barchitta, M., Agodi, A., & Ferrante, M. (2017). B(a)P adduct levels and fertility: A cross‑sectional study in a Sicilian population. Molecular Medicine Reports, 15, 3398-3404. https://doi.org/10.3892/mmr.2017.6396
MLA
Oliveri Conti, G., Calogero, A. E., Giacone, F., Fiore, M., Barchitta, M., Agodi, A., Ferrante, M."B(a)P adduct levels and fertility: A cross‑sectional study in a Sicilian population". Molecular Medicine Reports 15.5 (2017): 3398-3404.
Chicago
Oliveri Conti, G., Calogero, A. E., Giacone, F., Fiore, M., Barchitta, M., Agodi, A., Ferrante, M."B(a)P adduct levels and fertility: A cross‑sectional study in a Sicilian population". Molecular Medicine Reports 15, no. 5 (2017): 3398-3404. https://doi.org/10.3892/mmr.2017.6396
Copy and paste a formatted citation
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Spandidos Publications style
Oliveri Conti G, Calogero AE, Giacone F, Fiore M, Barchitta M, Agodi A and Ferrante M: B(a)P adduct levels and fertility: A cross‑sectional study in a Sicilian population. Mol Med Rep 15: 3398-3404, 2017.
APA
Oliveri Conti, G., Calogero, A.E., Giacone, F., Fiore, M., Barchitta, M., Agodi, A., & Ferrante, M. (2017). B(a)P adduct levels and fertility: A cross‑sectional study in a Sicilian population. Molecular Medicine Reports, 15, 3398-3404. https://doi.org/10.3892/mmr.2017.6396
MLA
Oliveri Conti, G., Calogero, A. E., Giacone, F., Fiore, M., Barchitta, M., Agodi, A., Ferrante, M."B(a)P adduct levels and fertility: A cross‑sectional study in a Sicilian population". Molecular Medicine Reports 15.5 (2017): 3398-3404.
Chicago
Oliveri Conti, G., Calogero, A. E., Giacone, F., Fiore, M., Barchitta, M., Agodi, A., Ferrante, M."B(a)P adduct levels and fertility: A cross‑sectional study in a Sicilian population". Molecular Medicine Reports 15, no. 5 (2017): 3398-3404. https://doi.org/10.3892/mmr.2017.6396
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