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G protein, phosphorylated-GATA4 and VEGF expression in the hearts of transgenic mice overexpressing β1- and β2-adrenergic receptors

  • Authors:
    • Hyun‑Jin Tae
    • Natalia Petrashevskaya
    • In Hye Kim
    • Joon Ha Park
    • Jae‑Chul Lee
    • Moo‑Ho Won
    • Yang Hee Kim
    • Ji Hyeon Ahn
    • Jinseu Park
    • Soo Young Choi
    • Yong Hwan Jeon
  • View Affiliations / Copyright

    Affiliations: Bio‑Safety Research Institute, College of Veterinary Medicine, Chonbuk National University, Iksan, Jeollabuk 54596, Republic of Korea, Cardiopulmonary Genomics Program, Departments of Medicine and Physiology, University of Maryland School of Medicine, Baltimore, MD 21201, USA, Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea, Department of Surgery, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24289, Republic of Korea, Department of Biomedical Science, Research Institute of Bioscience and Biotechnology, Hallym University, Chuncheon, Gangwon 24252, Republic of Korea, Department of Radiology, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24289, Republic of Korea
    Copyright: © Tae et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4049-4054
    |
    Published online on: April 28, 2017
       https://doi.org/10.3892/mmr.2017.6526
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Abstract

β1- and β2-adrenergic receptors (ARs) regulate cardiac contractility, calcium handling and protein phosphorylation. The present study aimed to examine the expression levels of vascular endothelial growth factor A (VEGF‑A) and several G proteins, and the phosphorylation of transcription factor GATA binding protein 4 (GATA4), by western blot analysis, using isolated hearts from 6 month‑old transgenic (TG) mice that overexpress β1AR or β2AR. Cardiac contractility/relaxation and heart rate was increased in both β1AR TG and β2AR TG mouse hearts compared with wild type; however, no significant differences were observed between the β1‑ and β2AR TG mouse hearts. Protein expression levels of inhibitory guanine nucleotide‑binding protein (Gi) 2, Gi3 and G‑protein‑coupled receptor kinase 2 were upregulated in both TG mice, although the upregulation of Gi2 was more prominent in the β2AR TG mice. VEGF‑A expression levels were also increased in both TG mice, and were highest in the β1AR TG mice. In addition, the levels of phosphorylated‑GATA4 expression were increased in β1‑ and β2AR TG mice. In conclusion, the present study demonstrated that cardiac contractility/relaxation and heart rate is increased in β1AR TG and β2AR TG mice, and indicated that this increase may be related to the overexpression of G proteins and G‑protein‑associated proteins.
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1 

Cao S, Zeng Z, Wang X, Bin J, Xu D and Liao Y: Pravastatin slows the progression of heart failure by inhibiting the c-Jun N-terminal kinase-mediated intrinsic apoptotic signaling pathway. Mol Med Rep. 8:1163–1168. 2013.

2 

Sun JM, Wang CM, Guo Z, Hao YY, Xie YJ, Gu J and Wang AL: Reduction of isoproterenol-induced cardiac hypertrophy and modulation of myocardial connexin43 by a KATP channel agonist. Mol Med Rep. 11:1845–1850. 2015.

3 

Wang N, Cao Y and Zhu Y: Netrin-1 prevents the development of cardiac hypertrophy and heart failure. Mol Med Rep. 13:2175–2181. 2016.

4 

Pereira SB, Velloso MW, Chermont S, Quintão MM, Abdhala R Nunes, Giro C, Oliveira E, Alves T, Camacho V, De Fátima Maia Contarato L, Pena FM, et al: β-adrenergic receptor polymorphisms in susceptibility, response to treatment and prognosis in heart failure: Implication of ethnicity. Mol Med Rep. 7:259–265. 2013.

5 

Yu J, Yang S, Wang X and Gan R: Matrine improved the function of heart failure in rats via inhibiting apoptosis and blocking β3adrenoreceptor/endothelial nitric oxide synthase pathway. Mol Med Rep. 10:3199–3204. 2014.

6 

Liggett SB, Tepe NM, Lorenz JN, Canning AM, Jantz TD, Mitarai S, Yatani A and Dorn GW II: Early and delayed consequences of beta(2)-adrenergic receptor overexpression in mouse hearts: Critical role for expression level. Circulation. 101:1707–1714. 2000. View Article : Google Scholar

7 

Perez J Mialet, Rathz DA, Petrashevskaya NN, Hahn HS, Wagoner LE, Schwartz A, Dorn GW, Liggett SB, et al: Beta 1-adrenergic receptor polymorphisms confer differential function and predisposition to heart failure. Nat Med. 9:1300–1305. 2003. View Article : Google Scholar

8 

Xiao RP, Zhu W, Zheng M, Chakir K, Bond R, Lakatta EG and Cheng H: Subtype-specific beta-adrenoceptor signaling pathways in the heart and their potential clinical implications. Trends Pharmacol Sci. 25:358–365. 2004. View Article : Google Scholar

9 

Zaugg M, Xu W, Lucchinetti E, Shafiq SA, Jamali NZ and Siddiqui MA: Beta-adrenergic receptor subtypes differentially affect apoptosis in adult rat ventricular myocytes. Circulation. 102:344–350. 2000. View Article : Google Scholar

10 

Xiao RP, Cheng H, Zhou YY, Kuschel M and Lakatta EG: Recent advances in cardiac beta(2)-adrenergic signal transduction. Circ Res. 85:1092–1100. 1999. View Article : Google Scholar

11 

Hanft LM and McDonald KS: Sarcomere length dependence of power output is increased after PKA treatment in rat cardiac myocytes. Am J Physiol Heart Circ Physiol. 296:H1524–H1531. 2009. View Article : Google Scholar :

12 

Fredriksson JM, Lindquist JM, Bronnikov GE and Nedergaard J: Norepinephrine induces vascular endothelial growth factor gene expression in brown adipocytes through a beta-adrenoreceptor/cAMP/protein kinase a pathway involving src but independently of Erk1/2. J Biol Chem. 275:13802–13811. 2000. View Article : Google Scholar

13 

Tirziu D, Chorianopoulos E, Moodie KL, Palac RT, Zhuang ZW, Tjwa M, Roncal C, Eriksson U, Fu Q, Elfenbein A, et al: Myocardial hypertrophy in the absence of external stimuli is induced by angiogenesis in mice. J Clin Invest. 117:3188–3197. 2007. View Article : Google Scholar :

14 

Heineke J, Auger-Messier M, Xu J, Oka T, Sargent MA, York A, Klevitsky R, Vaikunth S, Duncan SA, Aronow BJ, et al: Cardiomyocyte GATA4 functions as a stress-responsive regulator of angiogenesis in the murine heart. J Clin Invest. 117:3198–3210. 2007. View Article : Google Scholar :

15 

Lee JC, Kim IH, Cho GS, Park JH, Ahn JH, Yan BC, Kwon HM, Kim YM, Cheon SH, Cho JH, et al: Ischemic preconditioning-induced neuroprotection against transient cerebral ischemic damage via attenuating ubiquitin aggregation. J Neurol Sci. 336:74–82. 2014. View Article : Google Scholar

16 

Dorn GW II, Tepe NM, Lorenz JN, Koch WJ and Liggett SB: Low- and high-level transgenic expression of beta2-adrenergic receptors differentially affect cardiac hypertrophy and function in Galphaq-overexpressing mice. Proc Natl Acad Sci USA. 96:pp. 6400–6405. 1999; View Article : Google Scholar :

17 

Du XJ, Autelitano DJ, Dilley RJ, Wang B, Dart AM and Woodcock EA: beta(2)-adrenergic receptor overexpression exacerbates development of heart failure after aortic stenosis. Circulation. 101:71–77. 2000. View Article : Google Scholar

18 

Du XJ, Gao XM, Jennings GL, Dart AM and Woodcock EA: Preserved ventricular contractility in infarcted mouse heart overexpressing beta (2)-adrenergic receptors. Am J Physiol Heart Circ Physiol. 279:H2456–H2463. 2000.

19 

Endoh M: Force-frequency relationship in intact mammalian ventricular myocardium: Physiological and pathophysiological relevance. Eur J Pharmacol. 500:73–86. 2004. View Article : Google Scholar

20 

Engelhardt S, Hein L, Dyachenkow V, Kranias EG, Isenberg G and Lohse MJ: Altered calcium handling is critically involved in the cardiotoxic effects of chronic beta-adrenergic stimulation. Circulation. 109:1154–1160. 2004. View Article : Google Scholar

21 

Foerster K, Groner F, Matthes J, Koch WJ, Birnbaumer L and Herzig S: Cardioprotection specific for the G protein Gi2 in chronic adrenergic signaling through beta 2-adrenoceptors. Proc Natl Acad Sci USA. 100:pp. 14475–14480. 2003; View Article : Google Scholar :

22 

Petrashevskaya N, Gaume BR, Mihlbachler KA, Dorn GW II and Liggett SB: Bitransgenesis with beta(2)-adrenergic receptors or adenylyl cyclase fails to improve beta(1)-adrenergic receptor cardiomyopathy. Clin Transl Sci. 1:221–227. 2008. View Article : Google Scholar

23 

Xu Q, Jennings NL, Sim K, Chang L, Gao XM, Kiriazis H, Lee YY, Nguyen MN, Woodcock EA, Zhang YY, et al: Pathological hypertrophy reverses β2-adrenergic receptor-induced angiogenesis in mouse heart. Physiol Rep. 3:pii: e123402015. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Tae HJ, Petrashevskaya N, Kim IH, Park JH, Lee JC, Won MH, Kim YH, Ahn JH, Park J, Choi SY, Choi SY, et al: G protein, phosphorylated-GATA4 and VEGF expression in the hearts of transgenic mice overexpressing β1- and β2-adrenergic receptors. Mol Med Rep 15: 4049-4054, 2017.
APA
Tae, H., Petrashevskaya, N., Kim, I.H., Park, J.H., Lee, J., Won, M. ... Jeon, Y.H. (2017). G protein, phosphorylated-GATA4 and VEGF expression in the hearts of transgenic mice overexpressing β1- and β2-adrenergic receptors. Molecular Medicine Reports, 15, 4049-4054. https://doi.org/10.3892/mmr.2017.6526
MLA
Tae, H., Petrashevskaya, N., Kim, I. H., Park, J. H., Lee, J., Won, M., Kim, Y. H., Ahn, J. H., Park, J., Choi, S. Y., Jeon, Y. H."G protein, phosphorylated-GATA4 and VEGF expression in the hearts of transgenic mice overexpressing β1- and β2-adrenergic receptors". Molecular Medicine Reports 15.6 (2017): 4049-4054.
Chicago
Tae, H., Petrashevskaya, N., Kim, I. H., Park, J. H., Lee, J., Won, M., Kim, Y. H., Ahn, J. H., Park, J., Choi, S. Y., Jeon, Y. H."G protein, phosphorylated-GATA4 and VEGF expression in the hearts of transgenic mice overexpressing β1- and β2-adrenergic receptors". Molecular Medicine Reports 15, no. 6 (2017): 4049-4054. https://doi.org/10.3892/mmr.2017.6526
Copy and paste a formatted citation
x
Spandidos Publications style
Tae HJ, Petrashevskaya N, Kim IH, Park JH, Lee JC, Won MH, Kim YH, Ahn JH, Park J, Choi SY, Choi SY, et al: G protein, phosphorylated-GATA4 and VEGF expression in the hearts of transgenic mice overexpressing β1- and β2-adrenergic receptors. Mol Med Rep 15: 4049-4054, 2017.
APA
Tae, H., Petrashevskaya, N., Kim, I.H., Park, J.H., Lee, J., Won, M. ... Jeon, Y.H. (2017). G protein, phosphorylated-GATA4 and VEGF expression in the hearts of transgenic mice overexpressing β1- and β2-adrenergic receptors. Molecular Medicine Reports, 15, 4049-4054. https://doi.org/10.3892/mmr.2017.6526
MLA
Tae, H., Petrashevskaya, N., Kim, I. H., Park, J. H., Lee, J., Won, M., Kim, Y. H., Ahn, J. H., Park, J., Choi, S. Y., Jeon, Y. H."G protein, phosphorylated-GATA4 and VEGF expression in the hearts of transgenic mice overexpressing β1- and β2-adrenergic receptors". Molecular Medicine Reports 15.6 (2017): 4049-4054.
Chicago
Tae, H., Petrashevskaya, N., Kim, I. H., Park, J. H., Lee, J., Won, M., Kim, Y. H., Ahn, J. H., Park, J., Choi, S. Y., Jeon, Y. H."G protein, phosphorylated-GATA4 and VEGF expression in the hearts of transgenic mice overexpressing β1- and β2-adrenergic receptors". Molecular Medicine Reports 15, no. 6 (2017): 4049-4054. https://doi.org/10.3892/mmr.2017.6526
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