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Article

Colchicine attenuates renal fibrosis in a murine unilateral ureteral obstruction model

  • Authors:
    • Seiji Itano
    • Minoru Satoh
    • Hiroyuki Kadoya
    • Yuji Sogawa
    • Atsushi Uchida
    • Tamaki Sasaki
    • Naoki Kashihara
  • View Affiliations / Copyright

    Affiliations: Department of Nephrology and Hypertension, Kawasaki Medical School, Kurashiki, Okayama 701‑0192, Japan
  • Pages: 4169-4175
    |
    Published online on: May 2, 2017
       https://doi.org/10.3892/mmr.2017.6539
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Abstract

The present study aimed to assess the effects of colchicine, a known anti‑inflammatory agent, on renal fibrosis using a murine model of unilateral ureteral obstruction (UUO). Male C57BL/6 mice were divided into two groups, vehicle‑ and colchicine‑treated. Colchicine (0.5 mg/kg/day) was administered by osmotic pump, and the UUO procedure was performed on the left kidney 7 days later. The mice were sacrificed at 14 days following UUO. Colchicine treatment suppressed interstitial fibrosis of the UUO kidneys. In addition, fibrogenic gene expression in the UUO kidneys was decreased by colchicine administration. NRK‑49F normal rat kidney fibroblasts were cultured with or without colchicine under angiotensin II stimulation, following which a wound‑healing assay and actin fiber staining were performed to evaluate the effects of colchicine in vitro. Colchicine was demonstrated to inhibit angiotensin II‑induced fibroblast migration in vitro in a concentration‑dependent manner. Colchicine treatment also suppressed the angiotensin II‑induced activation of Ras homolog gene family member A in NRK‑49F cells. In conclusion, colchicine treatment significantly inhibited fibroblast activity in vitro and attenuated renal fibrosis in vivo in UUO‑operated mice. Therefore, the prevention of renal fibrosis following injury may represent a novel therapeutic application for colchicine.
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1 

Liu Y: Cellular and molecular mechanisms of renal fibrosis. Nat Rev Nephrol. 7:684–696. 2011. View Article : Google Scholar :

2 

Zeisberg M and Neilson EG: Mechanisms of tubulointerstitial fibrosis. J Am Soc Nephrol. 21:1819–1834. 2010. View Article : Google Scholar

3 

Boor P, Sebekova K, Ostendorf T and Floege J: Treatment targets in renal fibrosis. Nephrol Dial Transplant. 22:3391–3407. 2007. View Article : Google Scholar

4 

Ramos AM, Gonzalez-Guerrero C, Sanz A, Sanchez-Niño MD, Rodríguez-Osorio L, Martín-Cleary C, Fernández-Fernández B, Ruiz-Ortega M and Ortiz A: Designing drugs that combat kidney damage. Expert Opin Drug Discov. 10:541–556. 2015. View Article : Google Scholar

5 

Ashburn TT and Thor KB: Drug repositioning: Identifying and developing new uses for existing drugs. Nat Rev Drug Discov. 3:673–683. 2004. View Article : Google Scholar

6 

Nuki G: Colchicine: Its mechanism of action and efficacy in crystal-induced inflammation. Curr Rheumatol Rep. 10:218–227. 2008. View Article : Google Scholar

7 

Hastie SB: Interactions of colchicine with tubulin. Pharmacol Ther. 51:377–401. 1991. View Article : Google Scholar

8 

Slobodnick A, Shah B, Pillinger MH and Krasnokutsky S: Colchicine: Old and new. Am J Med. 128:461–470. 2015. View Article : Google Scholar

9 

Rodríguez L, Cerbón-Ambriz J and Muñoz ML: Effects of colchicine and colchiceine in a biochemical model of liver injury and fibrosis. Arch Med Res. 29:109–116. 1998.

10 

Ledwozyw A: The effect of colchicine and vinblastine on bleomycin-induced lung fibrosis in rats. Acta Physiol Hung. 82:383–389. 1994.

11 

Disel U, Paydas S, Dogan A, Gulfiliz G and Yavuz S: Effect of colchicine on cyclosporine nephrotoxicity, reduction of TGF-beta overexpression, apoptosis, and oxidative damage: An experimental animal study. Transplant Proc. 36:1372–1376. 2004. View Article : Google Scholar

12 

McClurkin C Jr, Phan SH, Hsu CH, Patel SR, Spicker JK, Kshirsagar AM, Yuan WY and Wiggins RC: Moderate protection of renal function and reduction of fibrosis by colchicine in a model of anti-GBM disease in the rabbit. J Am Soc Nephrol. 1:257–265. 1990.

13 

Li JJ, Lee SH, Kim DK, Jin R, Jung DS, Kwak SJ, Kim SH, Han SH, Lee JE, Moon SJ, et al: Colchicine attenuates inflammatory cell infiltration and extracellular matrix accumulation in diabetic nephropathy. Am J Physiol Renal Physiol. 297:F200–F209. 2009. View Article : Google Scholar

14 

Guan T, Gao B, Chen G, Chen X, Janssen M, Uttarwar L, Ingram AJ and Krepinsky JC: Colchicine attenuates renal injury in a model of hypertensive chronic kidney disease. Am J Physiol Renal Physiol. 305:F1466–F1476. 2013. View Article : Google Scholar

15 

Klahr S and Pukerson ML: The pathophysiology of obstructive nephropathy: The role of vasoactive compounds in the hemodynamic and structural abnormalities of the obstructed kidney. Am J Kidney Dis. 23:219–223. 1994. View Article : Google Scholar

16 

Diamond JR, Kees-Folts D, Ding G, Frye JE and Restrepo NC: Macrophages, monocyte chemoattractant peptide-1 and TGF-beta 1 in experimental hydronephrosis. Am J Physiol. 266:F926–F933. 1994.

17 

Kaneto H, Morrissey J and Klahr S: Increased expression of TGF-beta 1 mRNA in the obstructed kidney of rats with unilateral ureteral ligation. Kidney Int. 44:313–321. 1993. View Article : Google Scholar

18 

Satoh M, Kashihara N, Yamasaki Y, Maruyama K, Okamoto K, Maeshima Y, Sugiyama H, Sugaya T, Murakami K and Makino H: Renal interstitial fibrosis is reduced in angiotensin II type 1a receptor-deficient mice. J Am Soc Nephrol. 12:317–325. 2001.

19 

Du F, Li S, Wang T, Zhang HY, Li DT, Du ZX and Wang HQ: Implication of Bcl-2-associated athanogene 3 in fibroblast growth factor-2-mediated epithelial-mesenchymal transition in renal epithelial cells. Exp Biol Med (Maywood). 240:566–575. 2015. View Article : Google Scholar :

20 

Satoh M, Fujimoto S, Horike H, Ozeki M, Nagasu H, Tomita N, Sasaki T and Kashihara N: Mitochondrial damage-induced impairment of angiogenesis in the aging rat kidney. Lab Invest. 91:190–202. 2011. View Article : Google Scholar

21 

Clark K, Karsch-Mizrachi I, Lipman DJ, Ostell J and Sayers EW: GenBank. Nucleic Acids Res. 44:D67–D72. 2016. View Article : Google Scholar

22 

Dhanasekaran S, Doherty TM and Kenneth J; TB Trials Study Group, : Comparison of different standards for real-time PCR-based absolute quantification. J Immunol Methods. 354:34–39. 2010. View Article : Google Scholar

23 

Bolick DT, Whetzel AM, Skaflen M, Deem TL, Lee J and Hedrick CC: Absence of the G protein-coupled receptor G2A in mice promotes monocyte/endothelial interactions in aorta. Circ Res. 100:572–580. 2007. View Article : Google Scholar

24 

Yurdakul S, Mat C, Tüzün Y, Ozyazgan Y, Hamuryudan V, Uysal O, Senocak M and Yazici H: A double-blind trial of colchicine in Behçet's syndrome. Arthritis Rheum. 44:2686–2692. 2001. View Article : Google Scholar

25 

Kaplan MM and Gershwin ME: Primary biliary cirrhosis. N Engl J Med. 353:1261–1273. 2005. View Article : Google Scholar

26 

La Regina M, Ben-Chetrit E, Gasparyan AY, Livneh A, Ozdogan H and Manna R: Current trends in colchicine treatment in familial Mediterranean fever. Clin Exp Rheumatol. 31(3 Suppl 77): S41–S46. 2013.

27 

Deftereos S, Giannopoulos G, Papoutsidakis N, Panagopoulou V, Kossyvakis C, Raisakis K, Cleman MW and Stefanadis C: Colchicine and the heart: Pushing the envelope. J Am Coll Cardiol. 62:1817–1825. 2013. View Article : Google Scholar

28 

Murali A and Rajalingam K: Small Rho GTPases in the control of cell shape and mobility. Cell Mol Life Sci. 71:1703–1721. 2014. View Article : Google Scholar

29 

Pellegrin S and Mellor H: Actin stress fibres. J Cell Sci. 120:3491–3499. 2007. View Article : Google Scholar

30 

Allen WE, Zicha D, Ridley AJ and Jones GE: A role for Cdc42 in macrophage chemotaxis. J Cell Biol. 141:1147–1157. 1998. View Article : Google Scholar :

31 

Takeda Y, Nishikimi T, Akimoto K, Matsuoka H and Ishimitsu T: Beneficial effects of a combination of Rho-kinase inhibitor and ACE inhibitor on tubulointerstitial fibrosis induced by unilateral ureteral obstruction. Hypertens Res. 33:965–973. 2010. View Article : Google Scholar

32 

Picard N, Baum O, Vogetseder A, Kaissling B and Le Hir M: Origin of renal myofibroblasts in the model of unilateral ureter obstruction in the rat. Histochem Cell Biol. 130:141–155. 2008. View Article : Google Scholar :

33 

Mack M and Yanagita M: Origin of myofibroblasts and cellular events triggering fibrosis. Kidney Int. 87:297–307. 2015. View Article : Google Scholar

34 

Zeisberg M and Kalluri R: The role of epithelial-to-mesenchymal transition in renal fibrosis. J Mol Med (Berl). 82:175–181. 2004. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Itano S, Satoh M, Kadoya H, Sogawa Y, Uchida A, Sasaki T and Kashihara N: Colchicine attenuates renal fibrosis in a murine unilateral ureteral obstruction model. Mol Med Rep 15: 4169-4175, 2017.
APA
Itano, S., Satoh, M., Kadoya, H., Sogawa, Y., Uchida, A., Sasaki, T., & Kashihara, N. (2017). Colchicine attenuates renal fibrosis in a murine unilateral ureteral obstruction model. Molecular Medicine Reports, 15, 4169-4175. https://doi.org/10.3892/mmr.2017.6539
MLA
Itano, S., Satoh, M., Kadoya, H., Sogawa, Y., Uchida, A., Sasaki, T., Kashihara, N."Colchicine attenuates renal fibrosis in a murine unilateral ureteral obstruction model". Molecular Medicine Reports 15.6 (2017): 4169-4175.
Chicago
Itano, S., Satoh, M., Kadoya, H., Sogawa, Y., Uchida, A., Sasaki, T., Kashihara, N."Colchicine attenuates renal fibrosis in a murine unilateral ureteral obstruction model". Molecular Medicine Reports 15, no. 6 (2017): 4169-4175. https://doi.org/10.3892/mmr.2017.6539
Copy and paste a formatted citation
x
Spandidos Publications style
Itano S, Satoh M, Kadoya H, Sogawa Y, Uchida A, Sasaki T and Kashihara N: Colchicine attenuates renal fibrosis in a murine unilateral ureteral obstruction model. Mol Med Rep 15: 4169-4175, 2017.
APA
Itano, S., Satoh, M., Kadoya, H., Sogawa, Y., Uchida, A., Sasaki, T., & Kashihara, N. (2017). Colchicine attenuates renal fibrosis in a murine unilateral ureteral obstruction model. Molecular Medicine Reports, 15, 4169-4175. https://doi.org/10.3892/mmr.2017.6539
MLA
Itano, S., Satoh, M., Kadoya, H., Sogawa, Y., Uchida, A., Sasaki, T., Kashihara, N."Colchicine attenuates renal fibrosis in a murine unilateral ureteral obstruction model". Molecular Medicine Reports 15.6 (2017): 4169-4175.
Chicago
Itano, S., Satoh, M., Kadoya, H., Sogawa, Y., Uchida, A., Sasaki, T., Kashihara, N."Colchicine attenuates renal fibrosis in a murine unilateral ureteral obstruction model". Molecular Medicine Reports 15, no. 6 (2017): 4169-4175. https://doi.org/10.3892/mmr.2017.6539
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