Flow control effect of necrostatin-1 on cell death of the NRK-52E renal tubular epithelial cell line

Luo,Jialun; Tao,Yiming; Liang,Xinling; Chen,Yuanhan; Zhang,Li; Jiang,Fen; Liu,Shuangxin; Ye,Zhiming; Li,Zhilian; Shi,Wei

doi:10.3892/mmr.2017.6556

July-2017 Volume 16 Issue 1

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July-2017 Volume 16 Issue 1

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Article Open Access

Flow control effect of necrostatin-1 on cell death of the NRK-52E renal tubular epithelial cell line

Authors:
- Jialun Luo
- Yiming Tao
- Xinling Liang
- Yuanhan Chen
- Li Zhang
- Fen Jiang
- Shuangxin Liu
- Zhiming Ye
- Zhilian Li
- Wei Shi
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Affiliations: Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Department of Nephrology, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510080, P.R. China, Department of Nephrology, The First Affiliated Hospital of Nanhua University, Hengyang, Hunan 421001, P.R. China

Copyright: © Luo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 57-62
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Published online on: May 9, 2017

https://doi.org/10.3892/mmr.2017.6556
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Abstract

Apoptosis and necroptosis occur in renal tubular epithelial cell (RTEC) death in acute kidney injury (AKI), and may be regulated by several methods. The present study identified a protective effect of necrostatin‑1 (Nec‑1) on RTECs via a flow-control-like effect. The results established a hypoxic‑ischemic injury model of rat NRK‑52E RTECs using tumour necrosis factor‑α followed by ATP depletion with antimycin A and the pan-caspase pathway blocker, benzyloxycarbonyl-Val-Ala-Asp-fluoro-methylketone. Following pre‑treatment of cells with Nec‑1, cell organelle inflation, fragmentation inhibition and improved cell viability were observed with a parallel reduced expression of microtubule‑associated protein 1A/1B‑light chain 3‑II. Nec‑1 was involved in flow control in the process of cell injury and death. In conclusion, the present study indicated that Nec‑1 provides a protective effect and serves an important role in the prevention of AKI in an NRK‑52E cell model. Further studies will be required to fully investigate the role of Nec‑1 in the development of AKI in vivo.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Flow control effect of necrostatin-1 on cell death of the NRK-52E renal tubular epithelial cell line

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