Expression of c-FLIP in a rat model of sepsis and its effects on endothelial apoptosis

Shen,Lei; Sun,Zhengda; Zhao,Feng; Wang,Wei; Zhang,Wenhong; Zhu,Hechen

doi:10.3892/mmr.2017.6564

July-2017 Volume 16 Issue 1

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July-2017 Volume 16 Issue 1

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Expression of c-FLIP in a rat model of sepsis and its effects on endothelial apoptosis

Authors:
- Lei Shen
- Zhengda Sun
- Feng Zhao
- Wei Wang
- Wenhong Zhang
- Hechen Zhu
View Affiliations / Copyright

Affiliations: Department of Intensive Care Unit, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China, Department of Radiology and Biomedical Imaging, University of California San Francisco, San Francisco, CA 94143, USA, Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China

Copyright: © Shen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 231-237
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Published online on: May 10, 2017

https://doi.org/10.3892/mmr.2017.6564
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Abstract

Sepsis is characterized by the impaired regulation of inflammatory responses. Apoptosis is important in the pathogenesis of sepsis. Cellular FLICE‑inhibitory protein (c‑FLIP) is a catalytically inactive caspase‑8 homologue, which negatively interferes with apoptotic signaling. The role of c‑FLIP in sepsis and in endothelial cell apoptosis, a critical step in the pathogenesis of sepsis, remains controversial. In the present study, to investigate the relationship between c‑FLIP and sepsis, a rat model of sepsis was induced by cecal ligation and puncture, and western blot analysis was used to detect the expression of c‑FLIPL, the long isoform of c‑FLIP. Lower protein expression levels of c‑FLIPL were found in the brain, intestine and lung of the rat sepsis model, compared with the rats in the sham surgery group. The association between the expression of c‑FLIPL and endothelial cell apoptosis was further examined in vitro by c‑FLIPL overexpression and flow cytometry, which demonstrated that the expression of c‑FLIPL was inversely correlated with endothelial cell apoptosis. These data suggested that c‑FLIP may be important in sepsis and shed light on therapeutic strategies.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Expression of c-FLIP in a rat model of sepsis and its effects on endothelial apoptosis

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