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Article

Neuropeptide Y suppresses epileptiform discharges by regulating AMPA receptor GluR2 subunit in rat hippocampal neurons

  • Authors:
    • Wei Bu
    • Wen‑Qing Zhao
    • Wen‑Ling Li
    • Chang‑Zheng Dong
    • Zhe Zhang
    • Qi‑Jun Li
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, The Third Hospital of Hebei Medical University, Shijiazhuang, Hebei 050051, P.R. China, Department of Neurosurgery, Hebei Medical University, Shijiazhuang, Hebei 050051, P.R. China, Department of Functional Neurosurgery, Hebei General Hospital, Shijiazhuang, Hebei 050051, P.R. China, Department of Orthopaedics, Shijiazhuang Third Hospital, Shijiazhuang, Hebei 050017, P.R. China
  • Pages: 387-395
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    Published online on: May 10, 2017
       https://doi.org/10.3892/mmr.2017.6567
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Abstract

The present study aimed to investigate the effects of neuropeptide Y (NPY) on the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor glutamate receptor 2 (GluR2) subunit in epileptiform discharge hippocampal neurons. Hippocampal neurons were harvested from neonatal Sprague‑Dawley rats aged <24 h and primarily cultured in vitro. At day 12 following culture, hippocampal neurons were divided into the following groups: Control, Mg2+‑free, NPY+Mg2+‑free and BIBP3226+NPY+Mg2+‑free. The action potential of neurons was measured using the whole cell patch clamp technique in the control, Mg2+‑free and NPY+Mg2+‑free groups. AMPA current (IAMPA) was detected and peak current density was calculated in each group. Alterations in total protein and phosphorylation of the GluR2 subunit were detected by western blot analysis, and GluR2 mRNA expression levels were detected by reverse transcription‑quantitative polymerase chain reaction, in each group. The whole cell patch clamp technique demonstrated an abnormal action potential in the Mg2+‑free group. The frequency and amplitude of the action potential were significantly greater in the Mg2+‑free group compared with the control group, and significantly reduced in the NPY+Mg2+‑free group compared with the Mg2+‑free group (P<0.05). In the Mg2+‑free group, compared with the control group, peak current density was significantly reduced (P<0.05), GluR2 subunit protein content was slightly reduced (P>0.05), phosphorylation levels of GluR2 subunit were significantly greater (P<0.05) and GluR2 mRNA was significantly reduced (P<0.05). In the NPY+Mg2+‑free group, compared with the Mg2+‑free group, peak current density was significantly greater (P<0.05), phosphorylation levels of GluR2 subunit were significantly reduced (P<0.05) and GluR2 mRNA expression was significantly greater (P<0.05). In the BIBP3226+NPY+Mg2+‑free group, compared with the NPY+Mg2+‑free group, peak current density was significantly reduced (P<0.05), phosphorylation levels of GluR2 subunit were significantly greater (P<0.05) and GluR2 mRNA expression was significantly reduced (P<0.05). After 3 h of treatment with Mg2+‑free extracellular fluid, epileptiform discharge was detected in the cells. NPY inhibited the discharge and its underlying mechanism may be that epileptiform discharge suppressed the function of the AMPA receptor GluR2 subunit. NPY relieved the inhibition of the GluR2 subunit via the Y1 receptor. This may provide a novel direction for future studies on the pathogenesis and treatment of epilepsy.
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Copy and paste a formatted citation
Spandidos Publications style
Bu W, Zhao WQ, Li WL, Dong CZ, Zhang Z and Li QJ: Neuropeptide Y suppresses epileptiform discharges by regulating AMPA receptor GluR2 subunit in rat hippocampal neurons. Mol Med Rep 16: 387-395, 2017.
APA
Bu, W., Zhao, W., Li, W., Dong, C., Zhang, Z., & Li, Q. (2017). Neuropeptide Y suppresses epileptiform discharges by regulating AMPA receptor GluR2 subunit in rat hippocampal neurons. Molecular Medicine Reports, 16, 387-395. https://doi.org/10.3892/mmr.2017.6567
MLA
Bu, W., Zhao, W., Li, W., Dong, C., Zhang, Z., Li, Q."Neuropeptide Y suppresses epileptiform discharges by regulating AMPA receptor GluR2 subunit in rat hippocampal neurons". Molecular Medicine Reports 16.1 (2017): 387-395.
Chicago
Bu, W., Zhao, W., Li, W., Dong, C., Zhang, Z., Li, Q."Neuropeptide Y suppresses epileptiform discharges by regulating AMPA receptor GluR2 subunit in rat hippocampal neurons". Molecular Medicine Reports 16, no. 1 (2017): 387-395. https://doi.org/10.3892/mmr.2017.6567
Copy and paste a formatted citation
x
Spandidos Publications style
Bu W, Zhao WQ, Li WL, Dong CZ, Zhang Z and Li QJ: Neuropeptide Y suppresses epileptiform discharges by regulating AMPA receptor GluR2 subunit in rat hippocampal neurons. Mol Med Rep 16: 387-395, 2017.
APA
Bu, W., Zhao, W., Li, W., Dong, C., Zhang, Z., & Li, Q. (2017). Neuropeptide Y suppresses epileptiform discharges by regulating AMPA receptor GluR2 subunit in rat hippocampal neurons. Molecular Medicine Reports, 16, 387-395. https://doi.org/10.3892/mmr.2017.6567
MLA
Bu, W., Zhao, W., Li, W., Dong, C., Zhang, Z., Li, Q."Neuropeptide Y suppresses epileptiform discharges by regulating AMPA receptor GluR2 subunit in rat hippocampal neurons". Molecular Medicine Reports 16.1 (2017): 387-395.
Chicago
Bu, W., Zhao, W., Li, W., Dong, C., Zhang, Z., Li, Q."Neuropeptide Y suppresses epileptiform discharges by regulating AMPA receptor GluR2 subunit in rat hippocampal neurons". Molecular Medicine Reports 16, no. 1 (2017): 387-395. https://doi.org/10.3892/mmr.2017.6567
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